Restriction map for
pF1KE0852
(Circular) (Six-base) MAPSORT of: pF1KE0852.seq Check: 1032 from: 1 to: 4007
pF1KE0852 4007 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 829 829
Size: 4007
AccI GT'mk_AC
Cuts at: 1045 1045
Size: 4007
Acc65I G'GTAC_C
Cuts at: 1023 1023
Size: 4007
AclI AA'CG_TT
Cuts at: 3847 3847
Size: 4007
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1832 2264 3011 3279 1832
Size: 432 747 268 2560
Fragments arranged by size:
2560 747 432 268
AfeI AGC'GCT
Cuts at: 828 828
Size: 4007
AflIII A'CryG_T
Cuts at: 1359 2463 3440 1359
Size: 1104 977 1926
Fragments arranged by size:
1926 1104 977
AgeI A'CCGG_T
Cuts at: 2395 2395
Size: 4007
AleI CACnn'nnGTG
Cuts at: 124 124
Size: 4007
AlwNI CAG_nnn'CTG
Cuts at: 1312 2879 1312
Size: 1567 2440
ApaLI G'TGCA_C
Cuts at: 2777 2777
Size: 4007
ApoI r'AATT_y
Cuts at: 133 1011 3360 3616 133
Size: 878 2349 256 524
Fragments arranged by size:
2349 878 524 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4007
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4007
AvaI C'yCGr_G
Cuts at: 1027 1027
Size: 4007
AvrII C'CTAG_G
Cuts at: 420 420
Size: 4007
BamHI G'GATC_C
Cuts at: 1032 1032
Size: 4007
BanI G'GyrC_C
Cuts at: 1023 1437 1685 1720 1023
Size: 414 248 35 3310
Fragments arranged by size:
3310 414 248 35
BanII G_rGCy'C
Cuts at: 1021 2051 1021
Size: 1030 2977
BbeI G_GCGC'C
Cuts at: 1441 1689 1441
Size: 248 3759
BciVI GTATCCnnnnn_n'
Cuts at: 1905 2666 4007 1905
Size: 761 1341 1905
Fragments arranged by size:
1905 1341 761
BclI T'GATC_A
Cuts at: 1527 1527
Size: 4007
BglII A'GATC_T
Cuts at: 1522 3559 1522
Size: 2037 1970
BlpI GC'TnA_GC
Cuts at: 789 1119 789
Size: 330 3677
Bme1580I G_kGCm'C
Cuts at: 1632 1725 2781 1632
Size: 93 1056 2858
Fragments arranged by size:
2858 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 887 1219 1300 1635 3779 887
Size: 332 81 335 2144 1115
Fragments arranged by size:
2144 1115 335 332 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2374 2374
Size: 4007
Bpu10I CC'TnA_GC
Cuts at: 681 681
Size: 4007
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1183 2062 2554 2852 1183
Size: 879 492 298 2338
Fragments arranged by size:
2338 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3711 3711
Size: 4007
BsaAI yAC'GTr
Cuts at: 1360 1360
Size: 4007
BsaBI GATnn'nnATC
Cuts at: 6 1521 3558 6
Size: 1515 2037 455
Fragments arranged by size:
2037 1515 455
BsaHI Gr'CG_yC
Cuts at: 1438 1686 2388 3884 1438
Size: 248 702 1496 1561
Fragments arranged by size:
1561 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1196 1717 2395 2669 2816 3227 1196
Size: 521 678 274 147 411 1976
Fragments arranged by size:
1976 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 186 243 186
Size: 57 3950
BseYI C'CCAG_C
Cuts at: 398 1432 2190 2767 3263 398
Size: 1034 758 577 496 1142
Fragments arranged by size:
1142 1034 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1549 1595 2803 104
Size: 1445 46 1208 1308
Fragments arranged by size:
1445 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1021 1799 1989 2781 1021
Size: 778 190 792 2247
Fragments arranged by size:
2247 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1021 1632 1725 1799 1989 2051 2781 1021
Size: 611 93 74 190 62 730 2247
Fragments arranged by size:
2247 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3227 3227
Size: 4007
BspHI T'CATG_A
Cuts at: 506 3183 506
Size: 2677 1330
BspMI ACCTGCnnnn'nnnn_
Cuts at: 829 1057 1271 1573 1954 829
Size: 228 214 302 381 2882
Fragments arranged by size:
2882 381 302 228 214
BsrBI CCG'CTC
Cuts at: 50 788 2299 2353 3837 4002 50
Size: 738 1511 54 1484 165 55
Fragments arranged by size:
1511 1484 738 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1919 1919
Size: 4007
BsrFI r'CCGG_y
Cuts at: 2005 2186 2395 2005
Size: 181 209 3617
Fragments arranged by size:
3617 209 181
BssSI C'ACGA_G
Cuts at: 2278 2636 2278
Size: 358 3649
BstAPI GCAn_nnn'nTGC
Cuts at: 1384 1384
Size: 4007
BstBI TT'CG_AA
Cuts at: 2368 2368
Size: 4007
BstEII G'GTnAC_C
Cuts at: 936 936
Size: 4007
BstYI r'GATC_y
Cuts at: 1 1032 1499 1522 1857 2103 3115 3201 3213
Size: 1031 467 23 335 246 1012 86 12
Cuts at: 3213 3559 1
Size: 346 449
Fragments arranged by size:
1031 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 970 970
Size: 4007
BtgI C'CryG_G
Cuts at: 536 536
Size: 4007
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1957 2138 2301 1957
Size: 181 163 3663
Fragments arranged by size:
3663 181 163
BtsI GCAGTG_nn'
Cuts at: 656 836 1250 656
Size: 180 414 3413
Fragments arranged by size:
3413 414 180
DraI TTT'AAA
Cuts at: 1006 1006
Size: 4007
DraIII CAC_nnn'GTG
Cuts at: 519 519
Size: 4007
DrdI GACnn_nn'nnGTC
Cuts at: 1713 2571 1713
Size: 858 3149
EaeI y'GGCC_r
Cuts at: 533 1592 1766 2157 2184 3529 533
Size: 1059 174 391 27 1345 1011
Fragments arranged by size:
1345 1059 1011 391 174 27
EagI C'GGCC_G
Cuts at: 1592 1592
Size: 4007
EarI CTCTTCn'nnn_
Cuts at: 327 2030 2240 327
Size: 1703 210 2094
Fragments arranged by size:
2094 1703 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2215 2525 2671 3817 2215
Size: 310 146 1146 2405
Fragments arranged by size:
2405 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1019 1019
Size: 4007
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1832 2264 3011 3279 1832
Size: 432 747 268 2560
Fragments arranged by size:
2560 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 165 542 1146 165
Size: 377 604 3026
Fragments arranged by size:
3026 604 377
EcoRI G'AATT_C
Cuts at: 133 1011 133
Size: 878 3129
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2026 2026
Size: 4007
FspI TGC'GCA
Cuts at: 1788 1788
Size: 4007
HaeII r_GCGC'y
Cuts at: 830 1441 1689 2711 3690 830
Size: 611 248 1022 979 1147
Fragments arranged by size:
1147 1022 979 611 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 485 517 564 596 973 1005 1849 1879 1881
Size: 32 47 32 377 32 844 30 2
Cuts at: 1881 1911 485
Size: 30 2581
Fragments arranged by size:
2581 844 377 47 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1046 1046
Size: 4007
KasI G'GCGC_C
Cuts at: 1437 1685 1437
Size: 248 3759
KpnI G_GTAC'C
Cuts at: 1027 1027
Size: 4007
MluI A'CGCG_T
Cuts at: 3440 3440
Size: 4007
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2678 2862 2678
Size: 184 3823
MscI TGG'CCA
Cuts at: 535 1768 535
Size: 1233 2774
MslI CAynn'nnrTG
Cuts at: 124 274 451 1996 2123 124
Size: 150 177 1545 127 2008
Fragments arranged by size:
2008 1545 177 150 127
MspA1I CmG'CkG
Cuts at: 807 1118 1432 1792 2805 3050 807
Size: 311 314 360 1013 245 1764
Fragments arranged by size:
1764 1013 360 314 311 245
NaeI GCC'GGC
Cuts at: 2188 2188
Size: 4007
NarI GG'CG_CC
Cuts at: 1438 1686 1438
Size: 248 3759
NdeI CA'TA_TG
Cuts at: 478 478
Size: 4007
NgoMIV G'CCGG_C
Cuts at: 2186 2186
Size: 4007
NspI r_CATG'y
Cuts at: 1060 1560 2467 1060
Size: 500 907 2600
Fragments arranged by size:
2600 907 500
PciI A'CATG_T
Cuts at: 2463 2463
Size: 4007
PmeI GTTT'AAAC
Cuts at: 1006 1006
Size: 4007
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3146 3178 3146
Size: 32 3975
PpuMI rG'GwC_Cy
Cuts at: 542 542
Size: 4007
PstI C_TGCA'G
Cuts at: 651 826 1054 1739 651
Size: 175 228 685 2919
Fragments arranged by size:
2919 685 228 175
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4007
PvuII CAG'CTG
Cuts at: 1432 1792 1432
Size: 360 3647
RsrII CG'GwC_CG
Cuts at: 2202 2202
Size: 4007
SacI G_AGCT'C
Cuts at: 1021 1021
Size: 4007
SalI G'TCGA_C
Cuts at: 1044 1044
Size: 4007
SapI GCTCTTCn'nnn_
Cuts at: 2030 2240 2030
Size: 210 3797
SbfI CC_TGCA'GG
Cuts at: 1054 1054
Size: 4007
ScaI AGT'ACT
Cuts at: 3524 3524
Size: 4007
SfcI C'TryA_G
Cuts at: 33 647 822 963 1050 1735 2728 2919 33
Size: 614 175 141 87 685 993 191 1121
Fragments arranged by size:
1121 993 685 614 191 175 141 87
SfoI GGC'GCC
Cuts at: 1439 1687 1439
Size: 248 3759
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4007
SmaI CCC'GGG
Cuts at: 1029 1029
Size: 4007
SmlI C'TyrA_G
Cuts at: 1162 2077 2569 2831 1162
Size: 915 492 262 2338
Fragments arranged by size:
2338 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4007
SphI G_CATG'C
Cuts at: 1060 1560 1060
Size: 500 3507
StyI C'CwwG_G
Cuts at: 420 1141 1495 420
Size: 721 354 2932
Fragments arranged by size:
2932 721 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1590 1723 2182 2391 1590
Size: 133 459 209 3206
Fragments arranged by size:
3206 459 209 133
TatI w'GTAC_w
Cuts at: 427 3522 427
Size: 3095 912
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3524 3556 3524
Size: 32 3975
Tth111I GACn'n_nGTC
Cuts at: 1804 1804
Size: 4007
XbaI T'CTAG_A
Cuts at: 1038 1038
Size: 4007
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 309 1502 309
Size: 1193 2814
XmaI C'CCGG_G
Cuts at: 1027 1027
Size: 4007
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BciVI BclI BglII BlpI
Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NdeI
NgoMIV NspI PciI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AlfI AloI ApaI AscI BaeI
BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI BpmI
BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstXI
BstZ17I ClaI CspCI EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI