Restriction map for
pF1KE0846
##################################
# NO CUT ENZYMES ( 170 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspI
AvaI AvaII AviII AvrII BalI
BamHI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstXI BstZ17I Bsu36I
Cac8I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MspA1I MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PstI
PvuI PvuII RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI StyI SwaI TfiI Tth111I
XbaI XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AciI 859 CCGC CCGC(-3/-1)
AcsI 31 GAATTC [GA]^AATT[CT]
AflIII 833 ACACGT A^C[GA][CT]GT
ApoI 31 GAATTC [GA]^AATT[CT]
AseI 520 ATTAAT AT^TAAT
AsnI 520 ATTAAT AT^TAAT
Asp700I 331 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 604 GGCACC G^G[CT][GA]CC
BanII 64 GAGCTC G[GA]GC[CT]^C
BcnI 546 CCCGG CC^[GC]GG
BpmI 67 CTCCAG CTGGAG(16/14)
BsmFI 325 GGGAC GGGAC(10/14)
BsrBI 859 CCGCTC CCGCTC(-3/-3)
BstEII 108 GGTGACC G^GT[ACGT]ACC
BstPI 108 GGTGACC G^GT[ACGT]ACC
BstYI 446 GGATCT [GA]^GATC[CT]
Cfr13I 508 GGCCC G^G[ACGT]CC
EcoO65I 108 GGTGACC G^GT[ACGT]ACC
EcoRI 31 GAATTC G^AATTC
HapII 547 CCGG C^CGG
HinfI 540 GACTC G^A[ACGT]TC
HpaII 547 CCGG C^CGG
MflI 446 GGATCT [GA]^GATC[CT]
MslI 735 CACATCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 547 CCGG C^CGG
NciI 546 CCCGG CC^[GC]GG
PflMI 499 CCATTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 540 GACTC GAGTC(4/5)
PshBI 520 ATTAAT AT^TAAT
SacI 64 GAGCTC GAGCT^C
Sau96I 508 GGCCC G^G[ACGT]CC
SfaNI 681 GATGC GCATC(5/9)
SfcI 867 CTATAG C^T[GA][CT]AG
SspI 435 AATATT AAT^ATT
StuI 707 AGGCCT AGG^CCT
TaqI 657 TCGA T^CGA
TthHB8I 657 TCGA T^CGA
Van91I 499 CCATTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 446 GGATCT [GA]^GATC[CT]
XmnI 331 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AfaI 177 GTAC GT^AC
550 GTAC
AspHI 64 GAGCTC G[AT]GC[AT]^C
250 GTGCTC
BbvI 75 GCAGC GCAGC(8/12)
370 GCTGC
BfaI 94 CTAG C^TAG
553 CTAG
BmyI 64 GAGCTC G[AGT]GC[ACT]^C
250 GTGCTC
BsiHKAI 64 GAGCTC G[AT]GC[AT]^C
250 GTGCTC
BsmAI 538 GAGAC GTCTC(1/5)
699 GAGAC
Bsp1286I 64 GAGCTC G[AGT]GC[ACT]^C
250 GTGCTC
BsrI 694 ACTGG ACTGG(1/-1)
397 CCAGT
EarI 529 CTCTTC CTCTTC(1/4)
891 GAAGAG
Fnu4HI 75 GCAGC GC^[ACGT]GC
370 GCTGC
HaeIII 508 GGCC GG^CC
708 GGCC
ItaI 75 GCAGC GC^[ACGT]GC
370 GCTGC
Ksp632I 529 CTCTTC CTCTTC(1/4)
891 GAAGAG
MaeI 94 CTAG C^TAG
553 CTAG
MaeII 167 ACGT A^CGT
835 ACGT
MaeIII 109 GTGAC ^GT[ACGT]AC
742 GTGAC
MseI 151 TTAA T^TAA
521 TTAA
RsaI 177 GTAC GT^AC
550 GTAC
Tru9I 151 TTAA T^TAA
521 TTAA
TseI 75 GCAGC G^C[AT]GC
370 GCTGC
Tsp45I 109 GTGAC ^GT[GC]AC
742 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AluI AlwI BsaJI BsgI BsiYI
BslI BssKI BstF5I BstNI DdeI
DpnII EcoRII FokI HphI MboI
MboII MnlI MvaI MwoI NdeII
NlaIII NlaIV Sau3AI ScrFI Tsp509I
TspRI