Restriction map for
pF1KE0846
(Circular) (Six-base) MAPSORT of: pF1KE0846.seq Check: 3580 from: 1 to: 4055
pF1KE0846 4055 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AccI GT'mk_AC
Cuts at: 1093 1093
Size: 4055
Acc65I G'GTAC_C
Cuts at: 1071 1071
Size: 4055
AclI AA'CG_TT
Cuts at: 3895 3895
Size: 4055
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 805 1880 2312 3059 3327 805
Size: 1075 432 747 268 1533
Fragments arranged by size:
1533 1075 747 432 268
AflIII A'CryG_T
Cuts at: 941 1407 2511 3488 941
Size: 466 1104 977 1508
Fragments arranged by size:
1508 1104 977 466
AgeI A'CCGG_T
Cuts at: 2443 2443
Size: 4055
AleI CACnn'nnGTG
Cuts at: 847 847
Size: 4055
AlwNI CAG_nnn'CTG
Cuts at: 1360 2927 1360
Size: 1567 2488
ApaLI G'TGCA_C
Cuts at: 2825 2825
Size: 4055
ApoI r'AATT_y
Cuts at: 139 1059 3408 3664 139
Size: 920 2349 256 530
Fragments arranged by size:
2349 920 530 256
AseI AT'TA_AT
Cuts at: 20 629 20
Size: 609 3446
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4055
AvaI C'yCGr_G
Cuts at: 1075 1075
Size: 4055
BamHI G'GATC_C
Cuts at: 1080 1080
Size: 4055
BanI G'GyrC_C
Cuts at: 712 1071 1485 1733 1768 712
Size: 359 414 248 35 2999
Fragments arranged by size:
2999 414 359 248 35
BanII G_rGCy'C
Cuts at: 176 1069 2099 176
Size: 893 1030 2132
Fragments arranged by size:
2132 1030 893
BbeI G_GCGC'C
Cuts at: 1489 1737 1489
Size: 248 3807
BciVI GTATCCnnnnn_n'
Cuts at: 1953 2714 4055 1953
Size: 761 1341 1953
Fragments arranged by size:
1953 1341 761
BclI T'GATC_A
Cuts at: 1575 1575
Size: 4055
BglII A'GATC_T
Cuts at: 1570 3607 1570
Size: 2037 2018
BlpI GC'TnA_GC
Cuts at: 1167 1167
Size: 4055
Bme1580I G_kGCm'C
Cuts at: 1680 1773 2829 1680
Size: 93 1056 2906
Fragments arranged by size:
2906 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 499 812 1267 1348 1683 3827 499
Size: 313 455 81 335 2144 727
Fragments arranged by size:
2144 727 455 335 313 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2422 2422
Size: 4055
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 160
Size: 4055
Bpu10I CC'TnA_GC
Cuts at: 170 170
Size: 4055
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1231 2110 2602 2900 1231
Size: 879 492 298 2386
Fragments arranged by size:
2386 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3759 3759
Size: 4055
BsaAI yAC'GTr
Cuts at: 1408 1408
Size: 4055
BsaBI GATnn'nnATC
Cuts at: 6 1569 3606 6
Size: 1563 2037 455
Fragments arranged by size:
2037 1563 455
BsaHI Gr'CG_yC
Cuts at: 1486 1734 2436 3932 1486
Size: 248 702 1496 1609
Fragments arranged by size:
1609 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1244 1765 2443 2717 2864 3275 1244
Size: 521 678 274 147 411 2024
Fragments arranged by size:
2024 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1480 2238 2815 3311 1480
Size: 758 577 496 2224
Fragments arranged by size:
2224 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 350 601 695 202
Size: 148 251 94 3562
Fragments arranged by size:
3562 251 148 94
BsiEI CG_ry'CG
Cuts at: 104 1597 1643 2851 104
Size: 1493 46 1208 1308
Fragments arranged by size:
1493 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 176 362 1069 1847 2037 2829 176
Size: 186 707 778 190 792 1402
Fragments arranged by size:
1402 792 778 707 190 186
Bsp1286I G_dGCh'C
Cuts at: 176 362 1069 1680 1773 1847 2037 2099 2829
Size: 186 707 611 93 74 190 62 730
Cuts at: 2829 176
Size: 1402
Fragments arranged by size:
1402 730 707 611 190 186 93 74
62
BspEI T'CCGG_A
Cuts at: 3275 3275
Size: 4055
BspHI T'CATG_A
Cuts at: 3231 3231
Size: 4055
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1105 1319 1621 2002 1105
Size: 214 302 381 3158
Fragments arranged by size:
3158 381 302 214
BsrBI CCG'CTC
Cuts at: 50 969 2347 2401 3885 4050 50
Size: 919 1378 54 1484 165 55
Fragments arranged by size:
1484 1378 919 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1967 1967
Size: 4055
BsrFI r'CCGG_y
Cuts at: 2053 2234 2443 2053
Size: 181 209 3665
Fragments arranged by size:
3665 209 181
BssSI C'ACGA_G
Cuts at: 2326 2684 2326
Size: 358 3697
BstAPI GCAn_nnn'nTGC
Cuts at: 1432 1432
Size: 4055
BstBI TT'CG_AA
Cuts at: 2416 2416
Size: 4055
BstEII G'GTnAC_C
Cuts at: 216 216
Size: 4055
BstYI r'GATC_y
Cuts at: 1 554 1080 1547 1570 1905 2151 3163 3249
Size: 553 526 467 23 335 246 1012 86
Cuts at: 3249 3261 3607 1
Size: 12 346 449
Fragments arranged by size:
1012 553 526 467 449 346 335 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 472 2005 2186 2349 472
Size: 1533 181 163 2178
Fragments arranged by size:
2178 1533 181 163
BtsI GCAGTG_nn'
Cuts at: 1298 1298
Size: 4055
DraI TTT'AAA
Cuts at: 1054 1054
Size: 4055
DrdI GACnn_nn'nnGTC
Cuts at: 1761 2619 1761
Size: 858 3197
EaeI y'GGCC_r
Cuts at: 1640 1814 2205 2232 3577 1640
Size: 174 391 27 1345 2118
Fragments arranged by size:
2118 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1640 1640
Size: 4055
EarI CTCTTCn'nnn_
Cuts at: 643 994 2078 2288 643
Size: 351 1084 210 2410
Fragments arranged by size:
2410 1084 351 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2263 2573 2719 3865 2263
Size: 310 146 1146 2453
Fragments arranged by size:
2453 1146 310 146
EcoICRI GAG'CTC
Cuts at: 174 1067 174
Size: 893 3162
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 805 1880 2312 3059 3327 160
Size: 645 1075 432 747 268 888
Fragments arranged by size:
1075 888 747 645 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1194 1194
Size: 4055
EcoRI G'AATT_C
Cuts at: 139 1059 139
Size: 920 3135
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2074 2074
Size: 4055
FspI TGC'GCA
Cuts at: 1836 1836
Size: 4055
HaeII r_GCGC'y
Cuts at: 1489 1737 2759 3738 1489
Size: 248 1022 979 1806
Fragments arranged by size:
1806 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1897 1927 1929 1959 1897
Size: 30 2 30 3993
Fragments arranged by size:
3993 30 30 2
HincII GTy'rAC
Cuts at: 1094 1094
Size: 4055
KasI G'GCGC_C
Cuts at: 1485 1733 1485
Size: 248 3807
KpnI G_GTAC'C
Cuts at: 1075 1075
Size: 4055
MluI A'CGCG_T
Cuts at: 3488 3488
Size: 4055
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2726 2910 2726
Size: 184 3871
MscI TGG'CCA
Cuts at: 1816 1816
Size: 4055
MslI CAynn'nnrTG
Cuts at: 847 2044 2171 847
Size: 1197 127 2731
Fragments arranged by size:
2731 1197 127
MspA1I CmG'CkG
Cuts at: 1166 1480 1840 2853 3098 1166
Size: 314 360 1013 245 2123
Fragments arranged by size:
2123 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2236 2236
Size: 4055
NarI GG'CG_CC
Cuts at: 1486 1734 1486
Size: 248 3807
NgoMIV G'CCGG_C
Cuts at: 2234 2234
Size: 4055
NspI r_CATG'y
Cuts at: 1108 1608 2515 1108
Size: 500 907 2648
Fragments arranged by size:
2648 907 500
PciI A'CATG_T
Cuts at: 2511 2511
Size: 4055
PflMI CCAn_nnn'nTGG
Cuts at: 613 613
Size: 4055
PmeI GTTT'AAAC
Cuts at: 1054 1054
Size: 4055
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3194 3226 3194
Size: 32 4023
PstI C_TGCA'G
Cuts at: 1102 1787 1102
Size: 685 3370
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4055
PvuII CAG'CTG
Cuts at: 1480 1840 1480
Size: 360 3695
RsrII CG'GwC_CG
Cuts at: 2250 2250
Size: 4055
SacI G_AGCT'C
Cuts at: 176 1069 176
Size: 893 3162
SalI G'TCGA_C
Cuts at: 1092 1092
Size: 4055
SapI GCTCTTCn'nnn_
Cuts at: 2078 2288 2078
Size: 210 3845
SbfI CC_TGCA'GG
Cuts at: 1102 1102
Size: 4055
ScaI AGT'ACT
Cuts at: 3572 3572
Size: 4055
SfcI C'TryA_G
Cuts at: 33 975 1098 1783 2776 2967 33
Size: 942 123 685 993 191 1121
Fragments arranged by size:
1121 993 942 685 191 123
SfoI GGC'GCC
Cuts at: 1487 1735 1487
Size: 248 3807
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4055
SmaI CCC'GGG
Cuts at: 1077 1077
Size: 4055
SmlI C'TyrA_G
Cuts at: 1210 2125 2617 2879 1210
Size: 915 492 262 2386
Fragments arranged by size:
2386 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4055
SphI G_CATG'C
Cuts at: 1108 1608 1108
Size: 500 3555
SspI AAT'ATT
Cuts at: 545 545
Size: 4055
StuI AGG'CCT
Cuts at: 817 817
Size: 4055
StyI C'CwwG_G
Cuts at: 1189 1543 1189
Size: 354 3701
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 485 1638 1771 2230 2439 485
Size: 1153 133 459 209 2101
Fragments arranged by size:
2101 1153 459 209 133
TatI w'GTAC_w
Cuts at: 3570 3570
Size: 4055
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3572 3604 3572
Size: 32 4023
Tth111I GACn'n_nGTC
Cuts at: 1852 1852
Size: 4055
XbaI T'CTAG_A
Cuts at: 1086 1086
Size: 4055
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1550 1550
Size: 4055
XmaI C'CCGG_G
Cuts at: 1075 1075
Size: 4055
XmnI GAAnn'nnTTC
Cuts at: 443 443
Size: 4055
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PflMI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BsaXI BseRI BsiWI BsmI BsmBI BsrGI
BssHII BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI
ZraI