Restriction map for pF1KE0843
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# NO CUT ENZYMES (  166 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvaI        AviII       AvrII       BalI        BanI        
BbeI        BbrPI       BcgI        BclI        BcnI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BsaAI       BsaHI       BsaWI       BseAI       
BsgI        BsiEI       BsiWI       BsmBI       BsmFI       
BsoBI       Bsp1407I    BspDI       BspEI       BspLU11I    
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstUI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      ClaI        CpoI        DrdI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       FauI        FbaI        FseI        FspI        
HaeII       HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
HpaII       KasI        KpnI        Ksp632I     KspI        
MaeII       MfeI        MluI        MluNI       MroI        
MscI        MspA1I      MspI        MunI        MvnI        
NaeI        NarI        NciI        NdeI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        PvuII       RsaI        RsrII       
SacII       SalI        SapI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        SspBI       
StuI        SwaI        TaqI        Tth111I     TthHB8I     
Van91I      XbaI        XhoI        XmaCI       XmaI        
XmnI        


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# ONE CUT ENZYMES (   41 enzymes) #
###################################

AcsI          186  AAATTC               [GA]^AATT[CT]
ApaI          527  GGGCCC               GGGCC^C
ApoI          186  AAATTC               [GA]^AATT[CT]
AspHI         837  GAGCTC               G[AT]GC[AT]^C
BamHI         176  GGATCC               G^GATCC
BbsI          727  GTCTTC               GAAGAC(2/6)
BbvCI         405  CCTCAGC              CCTCAGC(-5/-2)
BfaI          106  CTAG                 C^TAG
BpmI          167  CTGGAG               CTGGAG(16/14)
BpuAI         727  GTCTTC               GAAGAC(2/6)
BsaBI         471  GATGGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          240  GAGACC               GGTCTC(1/5)
BseRI         517  CTCCTC               GAGGAG(10/8)
BsiHKAI       837  GAGCTC               G[AT]GC[AT]^C
BsiYI         519  CCTCTGCTGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          519  CCTCTGCTGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI          546  GAATGC               GAATGC(1/-1)
BspHI         977  TCATGA               T^CATGA
BspMI         464  GCAGGT               ACCTGC(4/8)
BstAPI        410  GCACCATCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         904  CCAGGCCAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI          298  TTTAAA               TTT^AAA
DraII         527  GGGCCCC              [GA]G^G[ACGT]CC[CT]
DraIII        382  CACAGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I      527  GGGCCCC              [GA]G^G[ACGT]CC[CT]
EcoT22I       548  ATGCAT               ATGCA^T
HphI          834  GGTGA                GGTGA(8/7)
MaeI          106  CTAG                 C^TAG
MaeIII        919  GTGAC                ^GT[ACGT]AC
MamI          471  GATGGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI          819  CACCATCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI          410  GCACCATCTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NsiI          548  ATGCAT               ATGCA^T
NspI          657  ACATGC               [GA]CATG^[CT]
PleI          779  GACTC                GAGTC(4/5)
RcaI          977  TCATGA               T^CATGA
SacI          837  GAGCTC               GAGCT^C
SbfI         1013  CCTGCAGG             CCTGCA^GG
Sse8387I     1013  CCTGCAGG             CCTGCA^GG
SspI          338  AATATT               AAT^ATT
Tsp45I        919  GTGAC                ^GT[GC]AC


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

AvaII         288  GGTCC                G^G[AT]CC
              828  GGTCC               
BanII         527  GGGCCC               G[GA]GC[CT]^C
              837  GAGCTC              
BciVI         305  GGATAC               GTATCC(6/5)
              616  GGATAC              
BstYI         176  GGATCC               [GA]^GATC[CT]
              487  AGATCC              
Cac8I         955  GCACGC               GC[ACGT]^[ACGT]GC
             1012  GCCTGC              
MflI          176  GGATCC               [GA]^GATC[CT]
              487  AGATCC              
NcoI          284  CCATGG               C^CATGG
              722  CCATGG              
PstI          786  CTGCAG               CTGCA^G
             1014  CTGCAG              
SfaNI         550  GCATC                GCATC(5/9)
              634  GCATC               
XhoII         176  GGATCC               [GA]^GATC[CT]
              487  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   41 enzymes) #
#########################################

AluI        AlwI        BbvI        BmyI        BsaJI       
BsmAI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       Cfr13I      DdeI        DpnII       DsaI        
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HinfI       ItaI        MboI        MboII       MnlI        
MseI        MvaI        NdeII       NlaIII      NlaIV       
Sau3AI      Sau96I      ScrFI       SfcI        StyI        
TfiI        Tru9I       TseI        Tsp509I     TspRI       
XcmI        
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