Restriction map for pF1KE0840
##################################
# NO CUT ENZYMES (  149 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AviII       AvrII       BanI        
BanII       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BfrI        BglII       
BlnI        BlpI        BmyI        Bpu1102I    BpuAI       
BsaAI       BsaHI       BsaI        BsaWI       BseAI       
BsiEI       BsiHKAI     BsiWI       BsmAI       BsmBI       
BsmFI       BsmI        Bsp1286I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrFI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstXI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraIII      EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoRI       
EcoRV       EcoT22I     FauI        FbaI        FseI        
FspI        HaeII       HgaI        Hin1I       HindIII     
HpaI        KasI        KpnI        KspI        MfeI        
MluI        MroI        MunI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PshAI       Psp1406I    PstI        
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspI        StuI        SwaI        Tth111I     Van91I      
XbaI        XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccII          45  CGCG                 CG^CG
AseI          834  ATTAAT               AT^TAAT
AsnI          834  ATTAAT               AT^TAAT
AvaI           92  CCCGGG               C^[CT]CG[GA]G
AvaII          59  GGTCC                G^G[AT]CC
BalI          746  TGGCCA               TGG^CCA
BfaI          671  CTAG                 C^TAG
BpmI          529  CTGGAG               CTGGAG(16/14)
BsaBI         162  GATTTCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI         303  GAGGAG               GAGGAG(10/8)
BsoBI          92  CCCGGG               C^[CT]CG[GA]G
BspMI         325  ACCTGC               ACCTGC(4/8)
BsrDI         393  CATTGC               GCAATG(2/0)
BstEII        812  GGTTACC              G^GT[ACGT]ACC
BstPI         812  GGTTACC              G^GT[ACGT]ACC
BstUI          45  CGCG                 CG^CG
CfoI          227  GCGC                 GCG^C
Cfr13I         59  GGTCC                G^G[ACGT]CC
DraI          990  TTTAAA               TTT^AAA
DraII          58  GGGTCCT              [GA]G^G[ACGT]CC[CT]
DrdI          406  GACCCCCTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          946  CCATGG               C^C[GA][CT]GG
EaeI          746  TGGCCA               [CT]^GGCC[GA]
EarI          351  CTCTTC               CTCTTC(1/4)
EcoO109I       58  GGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I       812  GGTTACC              G^GT[ACGT]ACC
EcoT14I       946  CCATGG               C^C[AT][AT]GG
HhaI          227  GCGC                 GCG^C
HinP1I        227  GCGC                 G^CGC
HincII        597  GTTGAC               GT[CT]^[GA]AC
HindII        597  GTTGAC               GT[CT]^[GA]AC
Ksp632I       351  CTCTTC               CTCTTC(1/4)
MaeI          671  CTAG                 C^TAG
MaeII          50  ACGT                 A^CGT
MamI          162  GATTTCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII         352  TCTTC                GAAGA(8/7)
MluNI         746  TGGCCA               TGG^CCA
MscI          746  TGGCCA               TGG^CCA
MslI          456  CATCAGCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI           45  CGCG                 CG^CG
NcoI          946  CCATGG               C^CATGG
PpuMI          58  GGGTCCT              [GA]G^G[AT]CC[CT]
PshBI         834  ATTAAT               AT^TAAT
Sau96I         59  GGTCC                G^G[ACGT]CC
SmaI           92  CCCGGG               CCC^GGG
StyI          946  CCATGG               C^C[AT][AT]GG
TaqI          383  TCGA                 T^CGA
TspRI         788  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       383  TCGA                 T^CGA
XmaCI          92  CCCGGG               C^CCGGG
XmaI           92  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AciI          397  GCGG                 CCGC(-3/-1)
              491  GCGG                
AcsI          449  GAATTT               [GA]^AATT[CT]
             1014  GAATTT              
ApoI          449  GAATTT               [GA]^AATT[CT]
             1014  GAATTT              
BamHI          88  GGATCC               G^GATCC
              470  GGATCC              
BglI          214  GCCTCTCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              739  GCCAGAGTGGC         
BsgI          187  CTGCAC               GTGCAG(16/14)
               31  GTGCAG              
BsiYI          18  CCTGCTGGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              380  CCATCGACAGG         
BslI           18  CCTGCTGGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              380  CCATCGACAGG         
Bsp1407I      104  TGTACA               T^GTACA
              485  TGTACA              
BsrGI         104  TGTACA               T^GTACA
              485  TGTACA              
BstF5I         14  CATCC                GGATG(2/0)
              719  CATCC               
BstYI          88  GGATCC               [GA]^GATC[CT]
              470  GGATCC              
Cac8I         132  GCTGGC               GC[ACGT]^[ACGT]GC
              223  GCCTGC              
DdeI          723  CTCAG                C^T[ACGT]AG
              909  CTCAG               
DpnII          89  GATC                 ^GATC
              471  GATC                
FokI           14  CATCC                GGATG(9/13)
              719  CATCC               
HaeIII        222  GGCC                 GG^CC
              747  GGCC                
HapII          85  CCGG                 C^CGG
               93  CCGG                
HpaII          85  CCGG                 C^CGG
               93  CCGG                
MboI           89  GATC                 ^GATC
              471  GATC                
MflI           88  GGATCC               [GA]^GATC[CT]
              470  GGATCC              
MseI          835  TTAA                 T^TAA
              991  TTAA                
MspI           85  CCGG                 C^CGG
               93  CCGG                
NdeII          89  GATC                 ^GATC
              471  GATC                
PvuII          34  CAGCTG               CAG^CTG
              184  CAGCTG              
Sau3AI         89  GATC                 ^GATC
              471  GATC                
SspBI         104  TGTACA               T^GTACA
              485  TGTACA              
Tru9I         835  TTAA                 T^TAA
              991  TTAA                
Tsp45I        247  GTGAC                ^GT[GC]AC
              784  GTCAC               
XhoII          88  GGATCC               [GA]^GATC[CT]
              470  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   28 enzymes) #
#########################################

AfaI        AluI        AlwI        BbvI        BcnI        
BsaJI       BsrI        BssKI       BstNI       EcoRII      
Fnu4HI      HinfI       HphI        ItaI        MaeIII      
MnlI        MspA1I      MvaI        MwoI        NciI        
NlaIII      NlaIV       RsaI        ScrFI       SfaNI       
TfiI        TseI        Tsp509I     
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