Restriction map for pF1KE0838
##################################
# NO CUT ENZYMES (  178 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AgeI        
AhdI        AlwNI       AocI        Aor51HI     ApaI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvaI        AvaII       AviII       
AvrII       BalI        BanI        BanII       BbeI        
BbrPI       BbvCI       BcgI        BcnI        BfrI        
BglI        BlnI        BlpI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiWI       BsiYI       BslI        
BsmBI       BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BsrBI       BsrGI       BssHII      BssKI       
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstNI       BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr13I      ClaI        
CpoI        DraII       DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRII      EcoT22I     FauI        FseI        
FspI        HaeII       HaeIII      HgaI        HhaI        
Hin1I       HinP1I      HindIII     HinfI       HpaI        
HphI        KasI        KpnI        Ksp632I     KspI        
MaeII       MamI        MluI        MluNI       MroI        
MscI        MslI        MspA1I      MvaI        MvnI        
MwoI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshBI       Psp1406I    PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        Sau96I      SbfI        ScrFI       
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SphI        Sse8387I    
SspBI       StuI        SwaI        TaqI        TfiI        
Tth111I     TthHB8I     Van91I      XbaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AflIII        901  ACATGT               A^C[GA][CT]GT
Alw44I        388  GTGCAC               G^TGCAC
ApaLI         388  GTGCAC               G^TGCAC
AspHI         388  GTGCAC               G[AT]GC[AT]^C
BamHI         395  GGATCC               G^GATCC
BbsI          313  GTCTTC               GAAGAC(2/6)
BciVI          73  GGATAC               GTATCC(6/5)
BclI          173  TGATCA               T^GATCA
BglII         616  AGATCT               A^GATCT
BmyI          388  GTGCAC               G[AGT]GC[ACT]^C
BpmI           41  CTGGAG               CTGGAG(16/14)
BpuAI         313  GTCTTC               GAAGAC(2/6)
BsaI          668  GAGACC               GGTCTC(1/5)
BsaJI         833  CCTTGG               C^C[ACGT][ACGT]GG
BsiHKAI       388  GTGCAC               G[AT]GC[AT]^C
BsmFI         574  GTCCC                GGGAC(10/14)
BsmI          893  GAATGC               GAATGC(1/-1)
Bsp1286I      388  GTGCAC               G[AGT]GC[ACT]^C
BspLU11I      901  ACATGT               A^CATGT
BspMI         301  ACCTGC               ACCTGC(4/8)
BsrDI          78  CATTGC               GCAATG(2/0)
BsrFI         658  ACCGGC               [GA]^CCGG[CT]
Cac8I         916  GCCTGC               GC[ACGT]^[ACGT]GC
Cfr10I        658  ACCGGC               [GA]^CCGG[CT]
DraI          861  TTTAAA               TTT^AAA
EcoRI          46  GAATTC               G^AATTC
EcoRV         594  GATATC               GAT^ATC
EcoT14I       833  CCTTGG               C^C[AT][AT]GG
FbaI          173  TGATCA               T^GATCA
HapII         659  CCGG                 C^CGG
HpaII         659  CCGG                 C^CGG
MspI          659  CCGG                 C^CGG
NlaIV         395  GGATCC               GG[ACGT]^[ACGT]CC
NspI          901  ACATGT               [GA]CATG^[CT]
PshAI         882  GACATTTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI           11  CTGCAG               CTGCA^G
SfaNI         407  GATGC                GCATC(5/9)
SfcI           11  CTGCAG               C^T[GA][CT]AG
SpeI           84  ACTAGT               A^CTAGT
SspI           60  AATATT               AAT^ATT
StyI          833  CCTTGG               C^C[AT][AT]GG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AcsI           46  GAATTC               [GA]^AATT[CT]
              164  GAATTT              
AfaI          208  GTAC                 GT^AC
              451  GTAC                
AluI          769  AGCT                 AG^CT
              795  AGCT                
AlwI          396  GATCC                GGATC(4/5)
              395  GGATC               
ApoI           46  GAATTC               [GA]^AATT[CT]
              164  GAATTT              
BbvI          442  GCAGC                GCAGC(8/12)
              817  GCAGC               
BfaI           55  CTAG                 C^TAG
               85  CTAG                
BsmAI         627  GAGAC                GTCTC(1/5)
              668  GAGAC               
BstYI         395  GGATCC               [GA]^GATC[CT]
              616  AGATCT              
DdeI          454  CTGAG                C^T[ACGT]AG
              874  CTGAG               
Fnu4HI        442  GCAGC                GC^[ACGT]GC
              817  GCAGC               
HincII         88  GTCAAC               GT[CT]^[GA]AC
              130  GTTGAC              
HindII         88  GTCAAC               GT[CT]^[GA]AC
              130  GTTGAC              
ItaI          442  GCAGC                GC^[ACGT]GC
              817  GCAGC               
MaeI           55  CTAG                 C^TAG
               85  CTAG                
MaeIII        588  GTCAC                ^GT[ACGT]AC
              839  GTCAC               
MfeI          479  CAATTG               C^AATTG
              569  CAATTG              
MflI          395  GGATCC               [GA]^GATC[CT]
              616  AGATCT              
MnlI          341  CCTC                 CCTC(7/6)
              876  GAGG                
MunI          479  CAATTG               C^AATTG
              569  CAATTG              
RsaI          208  GTAC                 GT^AC
              451  GTAC                
ScaI          207  AGTACT               AGT^ACT
              450  AGTACT              
TseI          442  GCAGC                G^C[AT]GC
              817  GCAGC               
Tsp45I        588  GTCAC                ^GT[GC]AC
              839  GTCAC               
XcmI          349  CCACTTTTTCTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              416  CCATTTCCTTGTTGG     
XhoII         395  GGATCC               [GA]^GATC[CT]
              616  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   13 enzymes) #
#########################################

BsrI        BstF5I      DpnII       FokI        MboI        
MboII       MseI        NdeII       NlaIII      Sau3AI      
Tru9I       Tsp509I     TspRI       
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