Restriction map for pF1KE0827
##################################
# NO CUT ENZYMES (  177 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AciI        
AclI        AcyI        AflII       AgeI        AhdI        
AlwI        AlwNI       AocI        Aor51HI     ApaI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvaI        AvaII       AviII       
AvrII       BamHI       BanI        BanII       BbeI        
BbrPI       BbvCI       BcgI        BciVI       BclI        
BcnI        BfaI        BfrI        BglI        BlnI        
BlpI        Bpu1102I    BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsiEI       BsiWI       BsmBI       
BsmI        BsoBI       BspDI       BspEI       BsrBI       
BsrFI       BssHII      BssKI       BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstNI       BstPI       
BstUI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      ClaI        CpoI        DraI        DraII       
DraIII      DrdI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRII      EcoRV       
EcoT14I     EcoT22I     FauI        FbaI        FseI        
FspI        HaeII       HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        HpaII       KasI        KpnI        Ksp632I     
KspI        MaeI        MamI        MfeI        MluI        
MroI        MspA1I      MspI        MunI        MvaI        
MvnI        MwoI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        PvuII       RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        ScrFI       
SexAI       SfaNI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspI        StyI        SwaI        
Tth111I     Van91I      XbaI        XhoI        XmaCI       
XmaI        XmnI        


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# ONE CUT ENZYMES (   45 enzymes) #
###################################

AccI          420  GTCTAC               GT^[AC][GT]AC
Alw44I        273  GTGCAC               G^TGCAC
ApaLI         273  GTGCAC               G^TGCAC
AspHI         273  GTGCAC               G[AT]GC[AT]^C
BbsI          203  GTCTTC               GAAGAC(2/6)
BbvI          428  GCAGC                GCAGC(8/12)
BglII          41  AGATCT               A^GATCT
BmyI          273  GTGCAC               G[AGT]GC[ACT]^C
BpmI          158  CTGGAG               CTGGAG(16/14)
BpuAI         203  GTCTTC               GAAGAC(2/6)
BsaAI          76  TACGTG               [CT]AC^GT[GA]
BsaJI         443  CCGTGG               C^C[ACGT][ACGT]GG
BseRI         652  CTCCTC               GAGGAG(10/8)
BsgI           97  CTGCAC               GTGCAG(16/14)
BsiHKAI       273  GTGCAC               G[AT]GC[AT]^C
BsmAI         493  GTCTC                GTCTC(1/5)
BsmFI         648  GTCCC                GGGAC(10/14)
Bsp1286I      273  GTGCAC               G[AGT]GC[ACT]^C
Bsp1407I      197  TGTACA               T^GTACA
BspHI         113  TCATGA               T^CATGA
BspLU11I      248  ACATGT               A^CATGT
BspMI         424  ACCTGC               ACCTGC(4/8)
BsrDI         322  GCAATG               GCAATG(2/0)
BsrGI         197  TGTACA               T^GTACA
BstF5I        132  CATCC                GGATG(2/0)
BstXI          21  CCACTTGTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI          41  AGATCT               [GA]^GATC[CT]
DdeI           38  CTGAG                C^T[ACGT]AG
DsaI          443  CCGTGG               C^C[GA][CT]GG
Fnu4HI        428  GCAGC                GC^[ACGT]GC
FokI          132  CATCC                GGATG(9/13)
ItaI          428  GCAGC                GC^[ACGT]GC
MflI           41  AGATCT               [GA]^GATC[CT]
NlaIV         217  GGCCCC               GG[ACGT]^[ACGT]CC
NspI          248  ACATGT               [GA]CATG^[CT]
PleI          365  GAGTC                GAGTC(4/5)
PstI          426  CTGCAG               CTGCA^G
RcaI          113  TCATGA               T^CATGA
SspBI         197  TGTACA               T^GTACA
StuI          294  AGGCCT               AGG^CCT
TaqI          120  TCGA                 T^CGA
TseI          428  GCAGC                G^C[AT]GC
Tsp45I        245  GTGAC                ^GT[GC]AC
TthHB8I       120  TCGA                 T^CGA
XhoII          41  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AcsI          514  AAATTC               [GA]^AATT[CT]
              579  AAATTC              
AfaI          198  GTAC                 GT^AC
              252  GTAC                
AflIII        248  ACATGT               A^C[GA][CT]GT
              569  ACACGT              
ApoI          514  AAATTC               [GA]^AATT[CT]
              579  AAATTC              
BalI           80  TGGCCA               TGG^CCA
              149  TGGCCA              
BsrI          157  ACTGG                ACTGG(1/-1)
              267  ACTGG               
Cfr13I          3  GGGCC                G^G[ACGT]CC
              217  GGCCC               
DpnII          42  GATC                 ^GATC
               70  GATC                
EaeI           80  TGGCCA               [CT]^GGCC[GA]
              149  TGGCCA              
HphI           57  GGTGA                GGTGA(8/7)
              440  TCACC               
MaeII          77  ACGT                 A^CGT
              571  ACGT                
MaeIII        245  GTGAC                ^GT[ACGT]AC
              520  GTTAC               
MboI           42  GATC                 ^GATC
               70  GATC                
MluNI          80  TGGCCA               TGG^CCA
              149  TGGCCA              
MscI           80  TGGCCA               TGG^CCA
              149  TGGCCA              
MseI          106  TTAA                 T^TAA
              383  TTAA                
MslI          438  CATCACCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              543  CACACCCATG          
NdeII          42  GATC                 ^GATC
               70  GATC                
RsaI          198  GTAC                 GT^AC
              252  GTAC                
Sau3AI         42  GATC                 ^GATC
               70  GATC                
Sau96I          3  GGGCC                G^G[ACGT]CC
              217  GGCCC               
TfiI          117  GAATC                G^A[AT]TC
              555  GAATC               
Tru9I         106  TTAA                 T^TAA
              383  TTAA                
XcmI           21  CCACTTGTTTGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              131  CCATCCTTCTGTTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   12 enzymes) #
#########################################

AluI        BsiYI       BslI        Cac8I       HaeIII      
HinfI       MboII       MnlI        NlaIII      SfcI        
Tsp509I     TspRI       
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