Restriction map for pF1KE0823
##################################
# NO CUT ENZYMES (  174 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        AlwI        AocI        Aor51HI     ApaI        
ApoI        AscI        AseI        AsnI        Asp700I     
AspEI       AspI        AvaI        AvaII       AviII       
AvrII       BamHI       BanII       BbeI        BbvCI       
BcgI        BciVI       BcnI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BsaBI       BsaHI       
BsaI        BsaJI       BsaWI       BseAI       BseRI       
BsiEI       BsiWI       BsmAI       BsmBI       BsmFI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstYI       
BstZ17I     Bsu36I      CelII       CfoI        Cfr10I      
ClaI        CpoI        DraI        DraII       DrdI        
DsaI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT14I     FauI        FseI        
FspI        HaeII       HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        HpaII       KasI        KspI        MamI        
MfeI        MflI        MluI        MroI        MseI        
MspA1I      MspI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmeI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        ScaI        SexAI       SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        SspBI       SspI        StuI        StyI        
SwaI        TaqI        Tru9I       Tsp509I     Tth111I     
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

Acc65I        415  GGTACC               G^GTACC
AciI          363  CCGC                 CCGC(-3/-1)
AluI          557  AGCT                 AG^CT
Alw44I        483  GTGCAC               G^TGCAC
AlwNI         238  CAGATTCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI         483  GTGCAC               G^TGCAC
Asp718I       415  GGTACC               G^GTACC
AspHI         483  GTGCAC               G[AT]GC[AT]^C
BanI          415  GGTACC               G^G[CT][GA]CC
BbrPI         480  CACGTG               CAC^GTG
BbsI           46  GTCTTC               GAAGAC(2/6)
BclI          134  TGATCA               T^GATCA
BglI          247  GCCACACTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI          483  GTGCAC               G[AGT]GC[ACT]^C
BpmI          410  CTCCAG               CTGGAG(16/14)
BpuAI          46  GTCTTC               GAAGAC(2/6)
BsaAI         480  CACGTG               [CT]AC^GT[GA]
BsiHKAI       483  GTGCAC               G[AT]GC[AT]^C
Bsp1286I      483  GTGCAC               G[AGT]GC[ACT]^C
BstXI         626  CCAATGTCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr13I        464  GGGCC                G^G[ACGT]CC
DraIII        327  CACAGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI           79  CTCTTC               CTCTTC(1/4)
EcoT22I       612  ATGCAT               ATGCA^T
FbaI          134  TGATCA               T^GATCA
KpnI          415  GGTACC               GGTAC^C
Ksp632I        79  CTCTTC               CTCTTC(1/4)
MaeII         481  ACGT                 A^CGT
NdeI          315  CATATG               CA^TATG
NsiI          612  ATGCAT               ATGCA^T
PleI          408  GACTC                GAGTC(4/5)
PmaCI         480  CACGTG               CAC^GTG
PmlI          480  CACGTG               CAC^GTG
Sau96I        464  GGGCC                G^G[ACGT]CC
SbfI          419  CCTGCAGG             CCTGCA^GG
SfiI          246  GGCCACACTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I      419  CCTGCAGG             CCTGCA^GG
TfiI          240  GATTC                G^A[AT]TC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AfaI          177  GTAC                 GT^AC
              416  GTAC                
BfaI           85  CTAG                 C^TAG
              622  CTAG                
BsiYI          60  CCCTGAACTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              671  CCATTTTGAGG         
BslI           60  CCCTGAACTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              671  CCATTTTGAGG         
BssKI         204  CCTGG                ^CC[ACGT]GG
              412  CCAGG               
BstNI         204  CCTGG                CC^[AT]GG
              412  CCAGG               
EcoRII        204  CCTGG                ^CC[AT]GG
              412  CCAGG               
HinfI         240  GATTC                G^A[ACGT]TC
              408  GACTC               
MaeI           85  CTAG                 C^TAG
              622  CTAG                
MslI          168  CACCCCCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              399  CATGACCATG          
MvaI          204  CCTGG                CC^[AT]GG
              412  CCAGG               
MwoI          211  GCCTGCTGTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              247  GCCACACTGGC         
NlaIV         415  GGTACC               GG[ACGT]^[ACGT]CC
              463  GGGGCC              
NspI          614  GCATGT               [GA]CATG^[CT]
              653  ACATGC              
PstI          420  CTGCAG               CTGCA^G
              564  CTGCAG              
RsaI          177  GTAC                 GT^AC
              416  GTAC                
ScrFI         204  CCTGG                CC^[ACGT]GG
              412  CCAGG               
SfaNI         154  GATGC                GCATC(5/9)
              208  GATGC               
SfcI          420  CTGCAG               C^T[GA][CT]AG
              564  CTGCAG              


#########################################
# 3 OR MORE CUT ENZYMES (   27 enzymes) #
#########################################

BalI        BbvI        BsgI        BspMI       BsrI        
BstF5I      Cac8I       DdeI        DpnII       EaeI        
Fnu4HI      FokI        HaeIII      HphI        ItaI        
MaeIII      MboI        MboII       MluNI       MnlI        
MscI        NdeII       NlaIII      Sau3AI      TseI        
Tsp45I      TspRI       
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