Restriction map for pF1KE0823
 (Linear) (Six-base) MAPSORT of: KIEE0823.seq  Check: 9585  from: 1  to: 699



KIEE0823.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:18  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     682     699 
   Size:        682      17
 
Acc65I G'GTAC_C

Cuts at:      0     415     699 
   Size:        415     284
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     574     699 
   Size:        574     125
 
AleI CACnn'nnGTG

Cuts at:      0     172     699 
   Size:        172     527
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     403     699 
   Size:        403     296
 
AlwNI CAG_nnn'CTG

Cuts at:      0     243     699 
   Size:        243     456
 
ApaLI G'TGCA_C

Cuts at:      0     483     699 
   Size:        483     216
 
BanI G'GyrC_C

Cuts at:      0     415     699 
   Size:        415     284
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      39     699 
   Size:         39     660
 
BclI T'GATC_A

Cuts at:      0     134     699 
   Size:        134     565
 
BfrBI ATG'CAT

Cuts at:      0     614     699 
   Size:        614      85
 
BglI GCCn_nnn'nGGC

Cuts at:      0     253     699 
   Size:        253     446
 
Bme1580I G_kGCm'C

Cuts at:      0     487     699 
   Size:        487     212
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     395     699 
   Size:        395     304
 
Bpu10I CC'TnA_GC

Cuts at:      0     187     699 
   Size:        187     512
 
BsaAI yAC'GTr

Cuts at:      0     482     699 
   Size:        482     217
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     393     423     699 
   Size:        393      30     276

  Fragments arranged by size: 

                 393     276      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     316     341     373     699 
   Size:        316      25      32     326

  Fragments arranged by size: 

                 326     316      32      25
 
BsiHKAI G_wGCw'C

Cuts at:      0     487     699 
   Size:        487     212
 
Bsp1286I G_dGCh'C

Cuts at:      0     487     699 
   Size:        487     212
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     347     427     682     699 
   Size:        347      80     255      17

  Fragments arranged by size: 

                 347     255      80      17
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     633     699 
   Size:        633      66
 
BtsI GCAGTG_nn'

Cuts at:      0     385     699 
   Size:        385     314
 
DraIII CAC_nnn'GTG

Cuts at:      0     332     699 
   Size:        332     367
 
EaeI y'GGCC_r

Cuts at:      0       2     245     254     434     699 
   Size:          2     243       9     180     265

  Fragments arranged by size: 

                 265     243     180       9       2
 
EarI CTCTTCn'nnn_

Cuts at:      0      85     699 
   Size:         85     614
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     395     574     699 
   Size:        395     179     125

  Fragments arranged by size: 

                 395     179     125
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     393     425     699 
   Size:        393      32     274

  Fragments arranged by size: 

                 393     274      32
 
KpnI G_GTAC'C

Cuts at:      0     419     699 
   Size:        419     280
 
MscI TGG'CCA

Cuts at:      0       4     247     256     436     699 
   Size:          4     243       9     180     263

  Fragments arranged by size: 

                 263     243     180       9       4
 
MslI CAynn'nnrTG

Cuts at:      0     172     403     699 
   Size:        172     231     296

  Fragments arranged by size: 

                 296     231     172
 
NdeI CA'TA_TG

Cuts at:      0     316     699 
   Size:        316     383
 
NsiI A_TGCA'T

Cuts at:      0     616     699 
   Size:        616      83
 
NspI r_CATG'y

Cuts at:      0     618     657     699 
   Size:        618      39      42

  Fragments arranged by size: 

                 618      42      39
 
PfoI T'CCnGG_A

Cuts at:      0     203     699 
   Size:        203     496
 
PmlI CAC'GTG

Cuts at:      0     482     699 
   Size:        482     217
 
PstI C_TGCA'G

Cuts at:      0     424     568     699 
   Size:        424     144     131

  Fragments arranged by size: 

                 424     144     131
 
SbfI CC_TGCA'GG

Cuts at:      0     424     699 
   Size:        424     275
 
SfcI C'TryA_G

Cuts at:      0     420     564     699 
   Size:        420     144     135

  Fragments arranged by size: 

                 420     144     135
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     253     699 
   Size:        253     446
 
TatI w'GTAC_w

Cuts at:      0     176     699 
   Size:        176     523
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI     AleI     AlfI    AlwNI    ApaLI     BanI
     BbsI     BclI    BfrBI     BglI Bme1580I     BpmI   Bpu10I    BsaAI
    BsaXI     BsgI  BsiHKAI Bsp1286I    BspMI    BstXI     BtsI   DraIII
     EaeI     EarI  Eco57MI    Hin4I     KpnI     MscI     MslI     NdeI
     NsiI     NspI     PfoI     PmlI     PstI     SbfI     SfcI     SfiI
     TatI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AloI     ApaI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI    BanII     BbeI    BbvCI     BcgI    BciVI    BglII
     BlpI    BmgBI     BmrI     BmtI     BplI    BpuEI     BsaI    BsaBI
    BsaHI    BsaWI    BseRI    BseYI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI    CspCI     DraI     DrdI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     MfeI     MluI     MmeI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI     PacI
     PasI     PciI    PflMI     PmeI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     ScaI    SexAI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI
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