Restriction map for pF1KE0821
##################################
# NO CUT ENZYMES (  173 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvaI        AviII       AvrII       BamHI       
BanI        BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BcnI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsgI        
BsiEI       BsiWI       BsmBI       BsoBI       BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstYI       BstZ17I     CelII       CfoI        
Cfr10I      ClaI        CpoI        DraI        DraII       
DraIII      EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
HpaII       KasI        KpnI        KspI        MaeII       
MaeIII      MamI        MflI        MluI        MroI        
MspA1I      MspI        MvnI        NaeI        NarI        
NciI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
SwaI        TaqI        Tsp45I      Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AluI          741  AGCT                 AG^CT
AlwI          693  GATCC                GGATC(4/5)
AocI          268  CCTAAGG              CC^T[ACGT]AGG
AspHI         571  GAGCAC               G[AT]GC[AT]^C
AvaII         291  GGTCC                G^G[AT]CC
BalI          380  TGGCCA               TGG^CCA
BanII         173  GAGCCC               G[GA]GC[CT]^C
BbvI          528  GCTGC                GCAGC(8/12)
BfaI          184  CTAG                 C^TAG
BseRI         777  CTCCTC               GAGGAG(10/8)
BsiHKAI       571  GAGCAC               G[AT]GC[AT]^C
BsmAI          22  GAGAC                GTCTC(1/5)
BsmI          667  GCATTC               GAATGC(1/-1)
Bsp1407I      146  TGTACA               T^GTACA
BsrGI         146  TGTACA               T^GTACA
BsrI          102  ACTGG                ACTGG(1/-1)
Bsu36I        268  CCTAAGG              CC^T[ACGT]AGG
DrdI          604  GACATAACTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          383  CCATGG               C^C[GA][CT]GG
EaeI          380  TGGCCA               [CT]^GGCC[GA]
Eco81I        268  CCTAAGG              CC^T[ACGT]AGG
EcoRV         241  GATATC               GAT^ATC
Fnu4HI        528  GCTGC                GC^[ACGT]GC
HphI           56  TCACC                GGTGA(8/7)
ItaI          528  GCTGC                GC^[ACGT]GC
MaeI          184  CTAG                 C^TAG
MfeI          589  CAATTG               C^AATTG
MluNI         380  TGGCCA               TGG^CCA
MscI          380  TGGCCA               TGG^CCA
MunI          589  CAATTG               C^AATTG
NcoI          383  CCATGG               C^CATGG
NspI           38  GCATGT               [GA]CATG^[CT]
PstI          155  CTGCAG               CTGCA^G
SfcI          155  CTGCAG               C^T[GA][CT]AG
SfiI          585  GGCCCAATTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI          420  CTTGAG               C^T[CT][GA]AG
SspBI         146  TGTACA               T^GTACA
StuI          272  AGGCCT               AGG^CCT
TfiI          371  GAATC                G^A[AT]TC
TseI          528  GCTGC                G^C[AT]GC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AfaI          147  GTAC                 GT^AC
              413  GTAC                
BbsI           11  GTCTTC               GAAGAC(2/6)
              214  GTCTTC              
BglI          352  GCCTTTGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              586  GCCCAATTGGC         
BmyI          173  GAGCCC               G[AGT]GC[ACT]^C
              571  GAGCAC              
BpmI          697  CTCCAG               CTGGAG(16/14)
              103  CTGGAG              
BpuAI          11  GTCTTC               GAAGAC(2/6)
              214  GTCTTC              
Bsp1286I      173  GAGCCC               G[AGT]GC[ACT]^C
              571  GAGCAC              
Cfr13I        291  GGTCC                G^G[ACGT]CC
              585  GGCCC               
DpnII         282  GATC                 ^GATC
              693  GATC                
EarI          780  CTCTTC               CTCTTC(1/4)
              522  GAAGAG              
Ksp632I       780  CTCTTC               CTCTTC(1/4)
              522  GAAGAG              
MboI          282  GATC                 ^GATC
              693  GATC                
MseI          439  TTAA                 T^TAA
              746  TTAA                
MslI          258  CACCACTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              576  CATTGGGGTG          
NdeII         282  GATC                 ^GATC
              693  GATC                
NlaIV         172  GGAGCC               GG[ACGT]^[ACGT]CC
              290  GGGTCC              
RsaI          147  GTAC                 GT^AC
              413  GTAC                
Sau3AI        282  GATC                 ^GATC
              693  GATC                
Sau96I        291  GGTCC                G^G[ACGT]CC
              585  GGCCC               
Tru9I         439  TTAA                 T^TAA
              746  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   25 enzymes) #
#########################################

BsaJI       BsiYI       BslI        BsmFI       BssKI       
BstF5I      BstNI       Cac8I       DdeI        EcoRII      
EcoT14I     FokI        HaeIII      HinfI       MboII       
MnlI        MvaI        MwoI        NlaIII      PleI        
ScrFI       SfaNI       StyI        Tsp509I     TspRI       
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