Restriction map for pF1KE0821
 (Linear) (Six-base) MAPSORT of: KIEE0821.seq  Check: 1185  from: 1  to: 810



KIEE0821.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:18  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     541     810 
   Size:        541     269
 
AleI CACnn'nnGTG

Cuts at:      0     262     810 
   Size:        262     548
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     191     223     810 
   Size:        191      32     587

  Fragments arranged by size: 

                 587     191      32
 
BanII G_rGCy'C

Cuts at:      0     177     810 
   Size:        177     633
 
BbsI GAAGACnn'nnnn_

Cuts at:      0       4     207     810 
   Size:          4     203     603

  Fragments arranged by size: 

                 603     203       4
 
BglI GCCn_nnn'nGGC

Cuts at:      0     358     592     810 
   Size:        358     234     218

  Fragments arranged by size: 

                 358     234     218
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     792     810 
   Size:        792      18
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     124     682     810 
   Size:        124     558     128

  Fragments arranged by size: 

                 558     128     124
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     441     810 
   Size:        441     369
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     191     221     810 
   Size:        191      30     589

  Fragments arranged by size: 

                 589     191      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     768     810 
   Size:        768      42
 
BseYI C'CCAG_C

Cuts at:      0     298     731     810 
   Size:        298     433      79

  Fragments arranged by size: 

                 433     298      79
 
BsiHKAI G_wGCw'C

Cuts at:      0     575     810 
   Size:        575     235
 
BsmI GAATG_Cn'

Cuts at:      0     667     810 
   Size:        667     143
 
Bsp1286I G_dGCh'C

Cuts at:      0     177     575     810 
   Size:        177     398     235

  Fragments arranged by size: 

                 398     235     177
 
BsrGI T'GTAC_A

Cuts at:      0     146     810 
   Size:        146     664
 
Bsu36I CC'TnA_GG

Cuts at:      0     269     810 
   Size:        269     541
 
BtgI C'CryG_G

Cuts at:      0     383     810 
   Size:        383     427
 
BtsI GCAGTG_nn'

Cuts at:      0     152     810 
   Size:        152     658
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     610     810 
   Size:        610     200
 
EaeI y'GGCC_r

Cuts at:      0     380     810 
   Size:        380     430
 
EarI CTCTTCn'nnn_

Cuts at:      0     517     786     810 
   Size:        517     269      24

  Fragments arranged by size: 

                 517     269      24
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     124     541     682     810 
   Size:        124     417     141     128

  Fragments arranged by size: 

                 417     141     128     124
 
EcoRV GAT'ATC

Cuts at:      0     243     810 
   Size:        243     567
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     164     196     810 
   Size:        164      32     614

  Fragments arranged by size: 

                 614     164      32
 
MfeI C'AATT_G

Cuts at:      0     589     810 
   Size:        589     221
 
MscI TGG'CCA

Cuts at:      0     382     810 
   Size:        382     428
 
MslI CAynn'nnrTG

Cuts at:      0     262     580     810 
   Size:        262     318     230

  Fragments arranged by size: 

                 318     262     230
 
NcoI C'CATG_G

Cuts at:      0     383     810 
   Size:        383     427
 
NspI r_CATG'y

Cuts at:      0      42     810 
   Size:         42     768
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     191     223     810 
   Size:        191      32     587

  Fragments arranged by size: 

                 587     191      32
 
PstI C_TGCA'G

Cuts at:      0     159     810 
   Size:        159     651
 
SfcI C'TryA_G

Cuts at:      0     155     810 
   Size:        155     655
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     592     810 
   Size:        592     218
 
SmlI C'TyrA_G

Cuts at:      0     420     810 
   Size:        420     390
 
StuI AGG'CCT

Cuts at:      0     274     810 
   Size:        274     536
 
StyI C'CwwG_G

Cuts at:      0      87     275     383     810 
   Size:         87     188     108     427

  Fragments arranged by size: 

                 427     188     108      87
 
TatI w'GTAC_w

Cuts at:      0     146     412     810 
   Size:        146     266     398

  Fragments arranged by size: 

                 398     266     146
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      51      83     810 
   Size:         51      32     727

  Fragments arranged by size: 

                 727      51      32
 

 Enzymes that do cut:

     AcuI     AleI     AloI    BanII     BbsI     BglI     BplI     BpmI
    BpuEI    BsaXI    BseRI    BseYI  BsiHKAI     BsmI Bsp1286I    BsrGI
   Bsu36I     BtgI     BtsI     DrdI     EaeI     EarI  Eco57MI    EcoRV
    Hin4I     MfeI     MscI     MslI     NcoI     NspI     PpiI     PstI
     SfcI     SfiI     SmlI     StuI     StyI     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I    BtgZI     ClaI    CspCI     DraI   DraIII
     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI     FalI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MluI     MmeI   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI    TaqII  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI
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