Restriction map for pF1KE0820
##################################
# NO CUT ENZYMES (  185 enzymes) #
##################################

Acc65I      AccI        AccII       AccIII      AclI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwI        AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BamHI       BanI        BanII       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BcnI        BfrI        BglI        BglII       
BlnI        BlpI        BmyI        BpmI        Bpu1102I    
BsaBI       BsaI        BsaWI       BseAI       BseRI       
BsiHKAI     BsiWI       BsmBI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspLU11I    
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      Cac8I       CelII       CfoI        Cfr10I      
ClaI        CpoI        DraI        DraIII      DrdI        
DsaI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRV       EcoT14I     EcoT22I     FbaI        FseI        
FspI        HaeII       HapII       HhaI        HinP1I      
HincII      HindII      HindIII     HinfI       HpaI        
HpaII       KasI        KpnI        Ksp632I     KspI        
MamI        MfeI        MflI        MluI        MroI        
MslI        MspI        MunI        MvnI        MwoI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TaqI        TfiI        
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   47 enzymes) #
###################################

AatII         163  GACGTC               GACGT^C
AcsI          447  GAATTC               [GA]^AATT[CT]
AcyI          163  GACGTC               G[GA]^CG[CT]C
AfaI          423  GTAC                 GT^AC
ApoI          447  GAATTC               [GA]^AATT[CT]
AvaII         275  GGTCC                G^G[AT]CC
BalI           20  TGGCCA               TGG^CCA
BbsI          336  GTCTTC               GAAGAC(2/6)
BfaI          565  CTAG                 C^TAG
BpuAI         336  GTCTTC               GAAGAC(2/6)
BsaAI          16  TACGTG               [CT]AC^GT[GA]
BsaHI         163  GACGTC               G[GA]^CG[CT]C
BsaJI         341  CCGAGG               C^C[ACGT][ACGT]GG
BsgI          351  GTGCAG               GTGCAG(16/14)
BsiEI           9  CGACCG               CG[GA][CT]^CG
BsmAI         472  GTCTC                GTCTC(1/5)
BsmFI         276  GTCCC                GGGAC(10/14)
BspHI         245  TCATGA               T^CATGA
BspMI         425  ACCTGC               ACCTGC(4/8)
BssKI         201  CCTGG                ^CC[ACGT]GG
BstNI         201  CCTGG                CC^[AT]GG
DdeI          526  CTGAG                C^T[ACGT]AG
DpnII         249  GATC                 ^GATC
DraII         274  GGGTCCC              [GA]G^G[ACGT]CC[CT]
EaeI           20  TGGCCA               [CT]^GGCC[GA]
EcoO109I      274  GGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoRI         447  GAATTC               G^AATTC
EcoRII        201  CCTGG                ^CC[AT]GG
HgaI           57  GACGC                GACGC(5/10)
Hin1I         163  GACGTC               G[GA]^CG[CT]C
HphI          392  TCACC                GGTGA(8/7)
MaeI          565  CTAG                 C^TAG
MaeIII        391  GTCAC                ^GT[ACGT]AC
MboI          249  GATC                 ^GATC
MluNI          20  TGGCCA               TGG^CCA
MscI           20  TGGCCA               TGG^CCA
MseI          506  TTAA                 T^TAA
MvaI          201  CCTGG                CC^[AT]GG
NdeII         249  GATC                 ^GATC
PpuMI         274  GGGTCCC              [GA]G^G[AT]CC[CT]
PvuII         142  CAGCTG               CAG^CTG
RcaI          245  TCATGA               T^CATGA
RsaI          423  GTAC                 GT^AC
Sau3AI        249  GATC                 ^GATC
ScrFI         201  CCTGG                CC^[ACGT]GG
Tru9I         506  TTAA                 T^TAA
Tsp45I        391  GTCAC                ^GT[GC]AC


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

BsrI          271  ACTGG                ACTGG(1/-1)
              333  CCAGT               
Cfr13I        204  GGCCC                G^G[ACGT]CC
              275  GGTCC               
FauI           63  GCGGG                CCCGC(4/6)
              442  GCGGG               
HaeIII         21  GGCC                 GG^CC
              204  GGCC                
MspA1I         61  CAGCGG               C[AC]G^C[GT]G
              142  CAGCTG              
NlaIV         274  GGGTCC               GG[ACGT]^[ACGT]CC
              275  GGTCCC              
Sau96I        204  GGCCC                G^G[ACGT]CC
              275  GGTCC               
SfaNI         547  GATGC                GCATC(5/9)
              308  GCATC               
Tsp509I       448  AATT                 ^AATT
              495  AATT                
TspRI         270  CACTG                CA[GC]TG[ACGT][ACGT]^
              384  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (   16 enzymes) #
#########################################

AciI        AluI        BbvI        BsiYI       BslI        
BstF5I      Fnu4HI      FokI        ItaI        MaeII       
MboII       MnlI        NlaIII      PstI        SfcI        
TseI        
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