Restriction map for pF1KE0817
##################################
# NO CUT ENZYMES (  182 enzymes) #
##################################

Acc65I      AccI        AccII       AccIII      AclI        
AcsI        AfaI        AflII       AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BclI        BcnI        BfrI        
BglI        BglII       BlnI        BlpI        BmyI        
Bpu1102I    BpuAI       BsaAI       BsaI        BsaWI       
BseAI       BsiEI       BsiHKAI     BsiWI       BsmFI       
BsmI        BsoBI       Bsp1286I    Bsp1407I    BspDI       
BspEI       BspHI       BsrBI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      Cac8I       CelII       CfoI        Cfr10I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT22I     FauI        
FbaI        FseI        FspI        HaeII       HapII       
HhaI        HinP1I      HincII      HindII      HindIII     
HpaI        HpaII       KasI        KpnI        KspI        
MluI        MluNI       MroI        MscI        MseI        
MspI        MvnI        NaeI        NarI        NciI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfaNI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TaqI        Tru9I       Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AatII         217  GACGTC               GACGT^C
AflIII        413  ACATGT               A^C[GA][CT]GT
AlwI          690  GATCC                GGATC(4/5)
AvaII          63  GGTCC                G^G[AT]CC
BciVI         673  GTATCC               GTATCC(6/5)
BfaI          196  CTAG                 C^TAG
BpmI          696  CTCCAG               CTGGAG(16/14)
BseRI          85  CTCCTC               GAGGAG(10/8)
BsgI          429  CTGCAC               GTGCAG(16/14)
BsiYI         698  CCAGTGAAGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          698  CCAGTGAAGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI         219  CGTCTC               CGTCTC(1/5)
BspLU11I      413  ACATGT               A^CATGT
BspMI         292  GCAGGT               ACCTGC(4/8)
BsrDI         528  CATTGC               GCAATG(2/0)
BssKI         351  CCTGG                ^CC[ACGT]GG
BstF5I        477  CATCC                GGATG(2/0)
BstNI         351  CCTGG                CC^[AT]GG
BstYI         689  AGATCC               [GA]^GATC[CT]
EcoRII        351  CCTGG                ^CC[AT]GG
FokI          477  CATCC                GGATG(9/13)
HgaI          422  GACGC                GACGC(5/10)
HphI            4  GGTGA                GGTGA(8/7)
MaeI          196  CTAG                 C^TAG
MaeII         218  ACGT                 A^CGT
MfeI          638  CAATTG               C^AATTG
MflI          689  AGATCC               [GA]^GATC[CT]
MspA1I        169  CAGCTG               C[AC]G^C[GT]G
MunI          638  CAATTG               C^AATTG
MvaI          351  CCTGG                CC^[AT]GG
NlaIV          62  GGGTCC               GG[ACGT]^[ACGT]CC
NspI          413  ACATGT               [GA]CATG^[CT]
PleI          524  GAGTC                GAGTC(4/5)
PvuII         169  CAGCTG               CAG^CTG
ScrFI         351  CCTGG                CC^[ACGT]GG
SfcI          111  CTATAG               C^T[GA][CT]AG
Tsp45I          5  GTGAC                ^GT[GC]AC
XhoII         689  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AciI           66  CCGC                 CCGC(-3/-1)
              372  CCGC                
AcyI          217  GACGTC               G[GA]^CG[CT]C
              422  GACGCC              
AluI          170  AGCT                 AG^CT
              733  AGCT                
BbvI          355  GCAGC                GCAGC(8/12)
              731  GCAGC               
BsaBI         144  GATGACAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              547  GATTCTTATC          
BsaHI         217  GACGTC               G[GA]^CG[CT]C
              422  GACGCC              
BsaJI         251  CCATGG               C^C[ACGT][ACGT]GG
              359  CCATGG              
BsmAI         220  GTCTC                GTCTC(1/5)
              283  GTCTC               
DpnII         108  GATC                 ^GATC
              690  GATC                
DsaI          251  CCATGG               C^C[GA][CT]GG
              359  CCATGG              
EarI           88  CTCTTC               CTCTTC(1/4)
              395  CTCTTC              
EcoT14I       251  CCATGG               C^C[AT][AT]GG
              359  CCATGG              
Fnu4HI        355  GCAGC                GC^[ACGT]GC
              731  GCAGC               
Hin1I         217  GACGTC               G[GA]^CG[CT]C
              422  GACGCC              
ItaI          355  GCAGC                GC^[ACGT]GC
              731  GCAGC               
Ksp632I        88  CTCTTC               CTCTTC(1/4)
              395  CTCTTC              
MamI          144  GATGACAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              547  GATTCTTATC          
MboI          108  GATC                 ^GATC
              690  GATC                
MwoI          241  GCTCCTAAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              355  GCAGCCATGGC         
NcoI          251  CCATGG               C^CATGG
              359  CCATGG              
NdeII         108  GATC                 ^GATC
              690  GATC                
Sau3AI        108  GATC                 ^GATC
              690  GATC                
StyI          251  CCATGG               C^C[AT][AT]GG
              359  CCATGG              
TfiI          163  GATTC                G^A[AT]TC
              547  GATTC               
TseI          355  GCAGC                G^C[AT]GC
              731  GCAGC               
Tsp509I       558  AATT                 ^AATT
              639  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   12 enzymes) #
#########################################

BsrI        Cfr13I      DdeI        HaeIII      HinfI       
MaeIII      MboII       MnlI        MslI        NlaIII      
Sau96I      TspRI       
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