Restriction map for pF1KE0816
 (Linear) (Six-base) MAPSORT of: KIEE0816.seq  Check: 4725  from: 1  to: 678



KIEE0816.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:18  ..

AccI GT'mk_AC

Cuts at:      0     101     542     678 
   Size:        101     441     136

  Fragments arranged by size: 

                 441     136     101
 
Acc65I G'GTAC_C

Cuts at:      0     365     678 
   Size:        365     313
 
AleI CACnn'nnGTG

Cuts at:      0     631     678 
   Size:        631      47
 
ApoI r'AATT_y

Cuts at:      0      65     249     678 
   Size:         65     184     429

  Fragments arranged by size: 

                 429     184      65
 
BanI G'GyrC_C

Cuts at:      0     365     678 
   Size:        365     313
 
BanII G_rGCy'C

Cuts at:      0     610     678 
   Size:        610      68
 
BclI T'GATC_A

Cuts at:      0     522     678 
   Size:        522     156
 
BglII A'GATC_T

Cuts at:      0     209     678 
   Size:        209     469
 
Bme1580I G_kGCm'C

Cuts at:      0     325     678 
   Size:        325     353
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     128     678 
   Size:        128     550
 
BsaAI yAC'GTr

Cuts at:      0     180     678 
   Size:        180     498
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     382     412     678 
   Size:        382      30     266

  Fragments arranged by size: 

                 382     266      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     161     210     373     678 
   Size:        161      49     163     305

  Fragments arranged by size: 

                 305     163     161      49
 
BsiHKAI G_wGCw'C

Cuts at:      0     185     610     665     678 
   Size:        185     425      55      13

  Fragments arranged by size: 

                 425     185      55      13
 
BsmI GAATG_Cn'

Cuts at:      0     337     678 
   Size:        337     341
 
Bsp1286I G_dGCh'C

Cuts at:      0     185     325     610     665     678 
   Size:        185     140     285      55      13

  Fragments arranged by size: 

                 285     185     140      55      13
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     232     678 
   Size:        232     446
 
BsrGI T'GTAC_A

Cuts at:      0     635     678 
   Size:        635      43
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     243     421     678 
   Size:        243     178     257

  Fragments arranged by size: 

                 257     243     178
 
BstYI r'GATC_y

Cuts at:      0     209     678 
   Size:        209     469
 
BtgI C'CryG_G

Cuts at:      0     629     678 
   Size:        629      49
 
BtsI GCAGTG_nn'

Cuts at:      0     149     385     678 
   Size:        149     236     293

  Fragments arranged by size: 

                 293     236     149
 
EaeI y'GGCC_r

Cuts at:      0     128     371     411     678 
   Size:        128     243      40     267

  Fragments arranged by size: 

                 267     243     128      40
 
EcoICRI GAG'CTC

Cuts at:      0     608     678 
   Size:        608      70
 
EcoO109I rG'GnC_Cy

Cuts at:      0     647     678 
   Size:        647      31
 
EcoRV GAT'ATC

Cuts at:      0      17     678 
   Size:         17     661
 
KpnI G_GTAC'C

Cuts at:      0     369     678 
   Size:        369     309
 
MscI TGG'CCA

Cuts at:      0     130     373     413     678 
   Size:        130     243      40     265

  Fragments arranged by size: 

                 265     243     130      40
 
MslI CAynn'nnrTG

Cuts at:      0     430     631     678 
   Size:        430     201      47

  Fragments arranged by size: 

                 430     201      47
 
NcoI C'CATG_G

Cuts at:      0     629     678 
   Size:        629      49
 
NspI r_CATG'y

Cuts at:      0     243     592     678 
   Size:        243     349      86

  Fragments arranged by size: 

                 349     243      86
 
PasI CC'CwG_GG

Cuts at:      0     318     678 
   Size:        318     360
 
PpuMI rG'GwC_Cy

Cuts at:      0     647     678 
   Size:        647      31
 
SacI G_AGCT'C

Cuts at:      0     610     678 
   Size:        610      68
 
StyI C'CwwG_G

Cuts at:      0     459     629     642     656     678 
   Size:        459     170      13      14      22

  Fragments arranged by size: 

                 459     170      22      14      13
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     398     678 
   Size:        398     280
 
TatI w'GTAC_w

Cuts at:      0     635     678 
   Size:        635      43
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     382     414     678 
   Size:        382      32     264

  Fragments arranged by size: 

                 382     264      32
 

 Enzymes that do cut:

     AccI   Acc65I     AleI     ApoI     BanI    BanII     BclI    BglII
 Bme1580I     BmrI    BsaAI    BsaXI     BsgI  BsiHKAI     BsmI Bsp1286I
    BspMI    BsrGI    BstXI    BstYI     BtgI     BtsI     EaeI  EcoICRI
 EcoO109I    EcoRV     KpnI     MscI     MslI     NcoI     NspI     PasI
    PpuMI     SacI     StyI    TaqII     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI
     BcgI    BciVI    BfrBI     BglI     BlpI    BmgBI     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BseRI
    BseYI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BsrBI    BsrDI
    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI
     EciI  Eco57MI    EcoNI    EcoRI     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     MfeI     MluI
     MmeI   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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