Restriction map for pF1KE0810
##################################
# NO CUT ENZYMES (  161 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     AspEI       
AspI        AvaI        AviII       AvrII       BamHI       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BfaI        BfrI        BglI        BglII       
BlnI        BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsiEI       BsiWI       BsiYI       
BslI        BsmBI       BsmFI       BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstUI       BstXI       
BstZ17I     Bsu36I      CfoI        Cfr10I      ClaI        
CpoI        DraII       DraIII      DrdI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoRV       EcoT22I     
FbaI        FseI        FspI        HaeII       HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        KasI        Ksp632I     KspI        MaeI        
MamI        MfeI        MluI        MroI        MunI        
MvnI        NaeI        NarI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfaNI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    SspBI       SspI        StuI        
SwaI        TaqI        Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

Acc65I        441  GGTACC               G^GTACC
AflIII        293  ACATGT               A^C[GA][CT]GT
Asp718I       441  GGTACC               G^GTACC
AspHI         490  GAGCAC               G[AT]GC[AT]^C
AvaII         180  GGTCC                G^G[AT]CC
BalI            6  TGGCCA               TGG^CCA
BanI          441  GGTACC               G^G[CT][GA]CC
BanII         665  GGGCTC               G[GA]GC[CT]^C
BlpI          667  GCTCAGC              GC^T[ACGT]AGC
BpmI           18  CTCCAG               CTGGAG(16/14)
Bpu1102I      667  GCTCAGC              GC^T[ACGT]AGC
BseRI         487  GAGGAG               GAGGAG(10/8)
BsiHKAI       490  GAGCAC               G[AT]GC[AT]^C
BsmAI         454  GTCTC                GTCTC(1/5)
BsmI          759  GCATTC               GAATGC(1/-1)
BspLU11I      293  ACATGT               A^CATGT
BstEII        330  GGTCACC              G^GT[ACGT]ACC
BstPI         330  GGTCACC              G^GT[ACGT]ACC
BstYI         734  GGATCT               [GA]^GATC[CT]
CelII         667  GCTCAGC              GC^T[ACGT]AGC
Cfr13I        180  GGTCC                G^G[ACGT]CC
DdeI          668  CTCAG                C^T[ACGT]AG
DraI          499  TTTAAA               TTT^AAA
DsaI          155  CCATGG               C^C[GA][CT]GG
EaeI            6  TGGCCA               [CT]^GGCC[GA]
EcoO65I       330  GGTCACC              G^GT[ACGT]ACC
EcoT14I       155  CCATGG               C^C[AT][AT]GG
FauI           89  GCGGG                CCCGC(4/6)
HaeIII          7  GGCC                 GG^CC
KpnI          441  GGTACC               GGTAC^C
MaeII         304  ACGT                 A^CGT
MflI          734  GGATCT               [GA]^GATC[CT]
MluNI           6  TGGCCA               TGG^CCA
MscI            6  TGGCCA               TGG^CCA
MspA1I        670  CAGCTG               C[AC]G^C[GT]G
NcoI          155  CCATGG               C^CATGG
NdeI          527  CATATG               CA^TATG
NlaIV         441  GGTACC               GG[ACGT]^[ACGT]CC
PvuII         670  CAGCTG               CAG^CTG
Sau96I        180  GGTCC                G^G[ACGT]CC
SmlI          807  CTTGAG               C^T[CT][GA]AG
SphI          390  GCATGC               GCATG^C
StyI          155  CCATGG               C^C[AT][AT]GG
XhoII         734  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AciI           89  GCGG                 CCGC(-3/-1)
              160  GCGG                
AfaI          297  GTAC                 GT^AC
              442  GTAC                
BcnI          140  CCCGG                CC^[GC]GG
              458  CCCGG               
BmyI          490  GAGCAC               G[AGT]GC[ACT]^C
              665  GGGCTC              
BsaJI         155  CCATGG               C^C[ACGT][ACGT]GG
              446  CCCTGG              
BsgI          283  CTGCAC               GTGCAG(16/14)
              622  GTGCAG              
Bsp1286I      490  GAGCAC               G[AGT]GC[ACT]^C
              665  GGGCTC              
BsrI          404  CCAGT                ACTGG(1/-1)
              723  CCAGT               
EcoRI          24  GAATTC               G^AATTC
              373  GAATTC              
HapII         141  CCGG                 C^CGG
              459  CCGG                
HgaI          420  GACGC                GACGC(5/10)
              756  GACGC               
HinfI         467  GAATC                G^A[ACGT]TC
              582  GATTC               
HpaII         141  CCGG                 C^CGG
              459  CCGG                
MspI          141  CCGG                 C^CGG
              459  CCGG                
NciI          140  CCCGG                CC^[GC]GG
              458  CCCGG               
RsaI          297  GTAC                 GT^AC
              442  GTAC                
SfcI           41  CTATAG               C^T[GA][CT]AG
              378  CTACAG              
TfiI          467  GAATC                G^A[AT]TC
              582  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (   35 enzymes) #
#########################################

AcsI        AluI        AlwI        ApoI        BbsI        
BbvI        BpuAI       BssKI       BstF5I      BstNI       
Cac8I       DpnII       EcoRII      Fnu4HI      FokI        
HphI        ItaI        MaeIII      MboI        MboII       
MnlI        MseI        MslI        MvaI        MwoI        
NdeII       NlaIII      NspI        Sau3AI      ScrFI       
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
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