Restriction map for pF1KE0810
 (Linear) (Six-base) MAPSORT of: KIEE0810.seq  Check: 4826  from: 1  to: 915



KIEE0810.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:17  ..

Acc65I G'GTAC_C

Cuts at:      0     441     915 
   Size:        441     474
 
AflIII A'CryG_T

Cuts at:      0     293     915 
   Size:        293     622
 
AleI CACnn'nnGTG

Cuts at:      0     529     915 
   Size:        529     386
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     782     843     915 
   Size:        782      61      72

  Fragments arranged by size: 

                 782      72      61
 
ApoI r'AATT_y

Cuts at:      0      24     265     373     502     915 
   Size:         24     241     108     129     413

  Fragments arranged by size: 

                 413     241     129     108      24
 
BanI G'GyrC_C

Cuts at:      0     441     915 
   Size:        441     474
 
BanII G_rGCy'C

Cuts at:      0     669     915 
   Size:        669     246
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     241     585     594     915 
   Size:        241     344       9     321

  Fragments arranged by size: 

                 344     321     241       9
 
BlpI GC'TnA_GC

Cuts at:      0     668     915 
   Size:        668     247
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       3     915 
   Size:          3     912
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     828     915 
   Size:        828      87
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0       1      31     915 
   Size:          1      30     884

  Fragments arranged by size: 

                 884      30       1
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     502     915 
   Size:        502     413
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     268     643     915 
   Size:        268     375     272

  Fragments arranged by size: 

                 375     272     268
 
BsiHKAI G_wGCw'C

Cuts at:      0     494     915 
   Size:        494     421
 
BsmI GAATG_Cn'

Cuts at:      0     759     915 
   Size:        759     156
 
Bsp1286I G_dGCh'C

Cuts at:      0     494     669     915 
   Size:        494     175     246

  Fragments arranged by size: 

                 494     246     175
 
BstEII G'GTnAC_C

Cuts at:      0     330     915 
   Size:        330     585
 
BstYI r'GATC_y

Cuts at:      0     734     915 
   Size:        734     181
 
BtgI C'CryG_G

Cuts at:      0     155     915 
   Size:        155     760
 
DraI TTT'AAA

Cuts at:      0     501     915 
   Size:        501     414
 
EaeI y'GGCC_r

Cuts at:      0       6     915 
   Size:          6     909
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       3     915 
   Size:          3     912
 
EcoRI G'AATT_C

Cuts at:      0      24     373     915 
   Size:         24     349     542

  Fragments arranged by size: 

                 542     349      24
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     329     361     915 
   Size:        329      32     554

  Fragments arranged by size: 

                 554     329      32
 
KpnI G_GTAC'C

Cuts at:      0     445     915 
   Size:        445     470
 
MscI TGG'CCA

Cuts at:      0       8     915 
   Size:          8     907
 
MslI CAynn'nnrTG

Cuts at:      0     292     529     610     764     915 
   Size:        292     237      81     154     151

  Fragments arranged by size: 

                 292     237     154     151      81
 
MspA1I CmG'CkG

Cuts at:      0     672     915 
   Size:        672     243
 
NcoI C'CATG_G

Cuts at:      0     155     915 
   Size:        155     760
 
NdeI CA'TA_TG

Cuts at:      0     528     915 
   Size:        528     387
 
NspI r_CATG'y

Cuts at:      0     297     394     417     915 
   Size:        297      97      23     498

  Fragments arranged by size: 

                 498     297      97      23
 
PciI A'CATG_T

Cuts at:      0     293     915 
   Size:        293     622
 
PfoI T'CCnGG_A

Cuts at:      0      19     915 
   Size:         19     896
 
PsiI TTA'TAA

Cuts at:      0     794     915 
   Size:        794     121
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     289     321     915 
   Size:        289      32     594

  Fragments arranged by size: 

                 594     289      32
 
PvuII CAG'CTG

Cuts at:      0     672     915 
   Size:        672     243
 
SfcI C'TryA_G

Cuts at:      0      41     378     915 
   Size:         41     337     537

  Fragments arranged by size: 

                 537     337      41
 
SmlI C'TyrA_G

Cuts at:      0     807     915 
   Size:        807     108
 
SphI G_CATG'C

Cuts at:      0     394     915 
   Size:        394     521
 
StyI C'CwwG_G

Cuts at:      0     155     915 
   Size:        155     760
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      88     915 
   Size:         88     827
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0       1      33     915 
   Size:          1      32     882

  Fragments arranged by size: 

                 882      32       1
 

 Enzymes that do cut:

   Acc65I   AflIII     AleI     AlfI     ApoI     BanI    BanII     BbsI
     BlpI     BpmI    BpuEI    BsaXI    BseRI     BsgI  BsiHKAI     BsmI
 Bsp1286I   BstEII    BstYI     BtgI     DraI     EaeI  Eco57MI    EcoRI
    Hin4I     KpnI     MscI     MslI   MspA1I     NcoI     NdeI     NspI
     PciI     PfoI     PsiI     PsrI    PvuII     SfcI     SmlI     SphI
     StyI    TaqII     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII Bme1580I    BmgBI     BmrI     BmtI
     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI
    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstXI  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI     EagI
     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     MfeI
     MluI     MmeI     NaeI     NarI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI    PflMI     PmeI     PmlI     PpiI    PpuMI
    PshAI   PspOMI    PspXI     PstI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI     StuI
     SwaI     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI
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