Restriction map for pF1KE0807
##################################
# NO CUT ENZYMES (  139 enzymes) #
##################################

AatII       Acc65I      AccII       AclI        AcyI        
AflII       AflIII      AgeI        AhdI        AlwI        
AocI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AviII       BamHI       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BfrI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsgI        BsiEI       BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspLU11I    
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        DsaI        EagI        Eam1105I    EclXI       
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FseI        FspI        HgaI        
Hin1I       HincII      HindII      HindIII     HpaI        
KasI        KpnI        KspI        MaeII       MaeIII      
MamI        MfeI        MluI        MseI        MunI        
MvnI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        RsrII       SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmlI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
SwaI        TaqI        TfiI        Tru9I       Tsp45I      
TthHB8I     XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccI          154  GTCTAC               GT^[AC][GT]AC
AccIII        780  TCCGGA               T^CCGGA
AcsI          621  GAATTT               [GA]^AATT[CT]
Aor51HI       242  AGCGCT               AGC^GCT
ApaI          524  GGGCCC               GGGCC^C
ApoI          621  GAATTT               [GA]^AATT[CT]
AspI          657  GACATTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI          585  TGGCCA               TGG^CCA
BanI          508  GGCACC               G^G[CT][GA]CC
BclI          723  TGATCA               T^GATCA
BglI          902  GCCAGTGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1075  AGATCT               A^GATCT
BsaI          121  GGTCTC               GGTCTC(1/5)
BseAI         780  TCCGGA               T^CCGGA
BsmAI         122  GTCTC                GTCTC(1/5)
BsmFI          80  GGGAC                GGGAC(10/14)
BspEI         780  TCCGGA               T^CCGGA
BspHI         744  TCATGA               T^CATGA
BstYI        1075  AGATCT               [GA]^GATC[CT]
CfoI          243  GCGC                 GCG^C
DraII         524  GGGCCCT              [GA]G^G[ACGT]CC[CT]
Eco47III      242  AGCGCT               AGC^GCT
EcoO109I      524  GGGCCCT              [GA]G^G[ACGT]CC[CT]
FbaI          723  TGATCA               T^GATCA
HaeII         242  AGCGCT               [GA]GCGC^[CT]
HhaI          243  GCGC                 GCG^C
HinP1I        243  GCGC                 G^CGC
HinfI         995  GAGTC                G^A[ACGT]TC
MflI         1075  AGATCT               [GA]^GATC[CT]
MluNI         585  TGGCCA               TGG^CCA
MroI          780  TCCGGA               T^CCGGA
MscI          585  TGGCCA               TGG^CCA
NspI          495  GCATGC               [GA]CATG^[CT]
PflFI         657  GACATTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI          995  GAGTC                GAGTC(4/5)
RcaI          744  TCATGA               T^CATGA
SacI           53  GAGCTC               GAGCT^C
SfcI          171  CTACAG               C^T[GA][CT]AG
SmaI           77  CCCGGG               CCC^GGG
SphI          495  GCATGC               GCATG^C
StuI          926  AGGCCT               AGG^CCT
Tsp509I       622  AATT                 ^AATT
Tth111I       657  GACATTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoII        1075  AGATCT               [GA]^GATC[CT]
XmaCI          77  CCCGGG               C^CCGGG
XmaI           77  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AciI          489  CCGC                 CCGC(-3/-1)
               38  GCGG                
AfaI          144  GTAC                 GT^AC
              967  GTAC                
Alw44I        376  GTGCAC               G^TGCAC
              436  GTGCAC              
AlwNI         541  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1009  CAGAAACTG           
ApaLI         376  GTGCAC               G^TGCAC
              436  GTGCAC              
AvaI           77  CCCGGG               C^[CT]CG[GA]G
              765  CCCGAG              
AvrII         189  CCTAGG               C^CTAGG
              447  CCTAGG              
BanII          53  GAGCTC               G[GA]GC[CT]^C
              524  GGGCCC              
BcnI           77  CCCGG                CC^[GC]GG
               78  CCGGG               
BlnI          189  CCTAGG               C^CTAGG
              447  CCTAGG              
BsaWI         482  ACCGGA               [AT]^CCGG[AT]
              780  TCCGGA              
BseRI        1003  CTCCTC               GAGGAG(10/8)
              734  GAGGAG              
BsoBI          77  CCCGGG               C^[CT]CG[GA]G
              765  CCCGAG              
BspMI         290  ACCTGC               ACCTGC(4/8)
              424  ACCTGC              
BstXI         260  CCACCTATCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              786  CCATCCTACTGG        
EaeI          284  TGGCCG               [CT]^GGCC[GA]
              585  TGGCCA              
EarI          806  CTCTTC               CTCTTC(1/4)
             1053  GAAGAG              
FauI          488  CCCGC                CCCGC(4/6)
               38  GCGGG               
Ksp632I       806  CTCTTC               CTCTTC(1/4)
             1053  GAAGAG              
MboII        1053  GAAGA                GAAGA(8/7)
              807  TCTTC               
MslI          429  CATCTCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              688  CATTTTGGTG          
NciI           77  CCCGG                CC^[GC]GG
               78  CCGGG               
PflMI         199  CCACTAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              239  CCAAGCGCTGG         
RsaI          144  GTAC                 GT^AC
              967  GTAC                
Van91I        199  CCACTAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              239  CCAAGCGCTGG         
XcmI          257  CCACCACCTATCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              518  CCACCTGGGCCCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   47 enzymes) #
#########################################

AluI        AspHI       AvaII       BbvI        BfaI        
BmyI        BpmI        BsaJI       BsiHKAI     BsiYI       
BslI        Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       Cac8I       Cfr13I      DdeI        DpnII       
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HpaII       HphI        ItaI        MaeI        
MboI        MnlI        MspA1I      MspI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       PvuII       
Sau3AI      Sau96I      ScrFI       SfaNI       StyI        
TseI        TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com