Restriction map for pF1KE0805
##################################
# NO CUT ENZYMES (  140 enzymes) #
##################################

AatII       Acc65I      AclI        AcsI        AflII       
AgeI        AhdI        AlwNI       AocI        Aor51HI     
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvrII       BamHI       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BcnI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BsiEI       
BsiWI       BsmBI       BsmFI       Bsp1407I    BspDI       
BsrBI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstYI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DdeI        DraI        DraIII      EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT14I     FauI        FbaI        FseI        HindIII     
HpaI        KasI        KpnI        Ksp632I     KspI        
MaeI        MfeI        MflI        MluI        MseI        
MspA1I      MunI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RsrII       SacI        SacII       SapI        SbfI        
ScaI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        Tru9I       Tth111I     XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccIII       1013  TCCGGA               T^CCGGA
AcyI          628  GGCGTC               G[GA]^CG[CT]C
AflIII        737  ACATGT               A^C[GA][CT]GT
Alw44I        733  GTGCAC               G^TGCAC
AlwI           91  GGATC                GGATC(4/5)
ApaI          875  GGGCCC               GGGCC^C
ApaLI         733  GTGCAC               G^TGCAC
AvaI          235  CCCGAG               C^[CT]CG[GA]G
AviII        1028  TGCGCA               TGC^GCA
BanI          721  GGCACC               G^G[CT][GA]CC
BbsI          223  GTCTTC               GAAGAC(2/6)
BpuAI         223  GTCTTC               GAAGAC(2/6)
BsaAI         730  TACGTG               [CT]AC^GT[GA]
BsaHI         628  GGCGTC               G[GA]^CG[CT]C
BsaI          391  GAGACC               GGTCTC(1/5)
BsaWI        1013  TCCGGA               [AT]^CCGG[AT]
BseAI        1013  TCCGGA               T^CCGGA
BseRI         712  CTCCTC               GAGGAG(10/8)
BsmI          114  GAATGC               GAATGC(1/-1)
BsoBI         235  CCCGAG               C^[CT]CG[GA]G
BspEI        1013  TCCGGA               T^CCGGA
BspLU11I      737  ACATGT               A^CATGT
BsrDI         109  GCAATG               GCAATG(2/0)
BsrI          441  CCAGT                ACTGG(1/-1)
BstAPI        109  GCAATGAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cac8I         128  GCCTGC               GC[ACGT]^[ACGT]GC
DrdI          532  GACAGGTACGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          296  CCGTGG               C^C[GA][CT]GG
EcoT22I       819  ATGCAT               ATGCA^T
FspI         1028  TGCGCA               TGC^GCA
HaeII         205  AGCGCC               [GA]GCGC^[CT]
HapII        1014  CCGG                 C^CGG
HgaI          629  GCGTC                GACGC(5/10)
Hin1I         628  GGCGTC               G[GA]^CG[CT]C
HincII        529  GTCGAC               GT[CT]^[GA]AC
HindII        529  GTCGAC               GT[CT]^[GA]AC
HpaII        1014  CCGG                 C^CGG
MroI         1013  TCCGGA               T^CCGGA
MspI         1014  CCGG                 C^CGG
NsiI          819  ATGCAT               ATGCA^T
PflMI         450  CCAGCACATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          472  GACTC                GAGTC(4/5)
PstI          342  CTGCAG               CTGCA^G
SalI          529  GTCGAC               G^TCGAC
SfcI          342  CTGCAG               C^T[GA][CT]AG
TfiI         1047  GATTC                G^A[AT]TC
Tsp509I      1025  AATT                 ^AATT
Van91I        450  CCAGCACATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AccI          529  GTCGAC               GT^[AC][GT]AC
              652  GTCTAC              
AccII         555  CGCG                 CG^CG
              752  CGCG                
AfaI          330  GTAC                 GT^AC
              537  GTAC                
AluI           58  AGCT                 AG^CT
               86  AGCT                
AspHI         166  GAGCAC               G[AT]GC[AT]^C
              733  GTGCAC              
BalI          518  TGGCCA               TGG^CCA
              608  TGGCCA              
BanII          79  GAGCCC               G[GA]GC[CT]^C
              875  GGGCCC              
BsaBI         402  GATCGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              480  GATCTGCATC          
BsgI          316  CTGCAC               GTGCAG(16/14)
              757  CTGCAC              
BsiHKAI       166  GAGCAC               G[AT]GC[AT]^C
              733  GTGCAC              
BsmAI         391  GAGAC                GTCTC(1/5)
              249  GTCTC               
BspHI         398  TCATGA               T^CATGA
              575  TCATGA              
BstF5I          6  CATCC                GGATG(2/0)
              279  CATCC               
BstUI         555  CGCG                 CG^CG
              752  CGCG                
BstXI         380  CCAATGCCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              845  CCATTCTCCTGG        
DraII         590  AGGCCCT              [GA]G^G[ACGT]CC[CT]
              875  GGGCCCC             
EcoO109I      590  AGGCCCT              [GA]G^G[ACGT]CC[CT]
              875  GGGCCCC             
FokI            6  CATCC                GGATG(9/13)
              279  CATCC               
HinfI         472  GACTC                G^A[ACGT]TC
             1047  GATTC               
MaeIII        541  GTCAC                ^GT[ACGT]AC
              835  GTCAC               
MamI          402  GATCGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              480  GATCTGCATC          
MluNI         518  TGGCCA               TGG^CCA
              608  TGGCCA              
MscI          518  TGGCCA               TGG^CCA
              608  TGGCCA              
MslI          576  CATGACCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              687  CATCACCATG          
MvnI          555  CGCG                 CG^CG
              752  CGCG                
NspI          362  ACATGC               [GA]CATG^[CT]
              737  ACATGT              
RcaI          398  TCATGA               T^CATGA
              575  TCATGA              
RsaI          330  GTAC                 GT^AC
              537  GTAC                
SexAI         893  ACCTGGT              A^CC[AT]GGT
              962  ACCTGGT             
Tsp45I        541  GTCAC                ^GT[GC]AC
              835  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   40 enzymes) #
#########################################

AciI        AvaII       BbvI        BmyI        BpmI        
BsaJI       BsiYI       BslI        Bsp1286I    BspMI       
BssKI       BstNI       CfoI        Cfr13I      DpnII       
EaeI        EcoRII      Fnu4HI      HaeIII      HhaI        
HinP1I      HphI        ItaI        MaeII       MboI        
MboII       MnlI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       Sau3AI      Sau96I      ScrFI       
SfaNI       TaqI        TseI        TspRI       TthHB8I     
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