Restriction map for pF1KE0805
 (Linear) (Six-base) MAPSORT of: KIEE0805.seq  Check: 7291  from: 1  to: 1080



KIEE0805.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:17  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     795     919    1080 
   Size:        795     124     161

  Fragments arranged by size: 

                 795     161     124
 
AccI GT'mk_AC

Cuts at:      0     530     653    1080 
   Size:        530     123     427

  Fragments arranged by size: 

                 530     427     123
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     171    1080 
   Size:        171     909
 
AflIII A'CryG_T

Cuts at:      0     737    1080 
   Size:        737     343
 
ApaI G_GGCC'C

Cuts at:      0     879    1080 
   Size:        879     201
 
ApaLI G'TGCA_C

Cuts at:      0     733    1080 
   Size:        733     347
 
AvaI C'yCGr_G

Cuts at:      0     235    1080 
   Size:        235     845
 
BanI G'GyrC_C

Cuts at:      0     721    1080 
   Size:        721     359
 
BanII G_rGCy'C

Cuts at:      0      83     879    1080 
   Size:         83     796     201

  Fragments arranged by size: 

                 796     201      83
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     216    1080 
   Size:        216     864
 
BfrBI ATG'CAT

Cuts at:      0     821    1080 
   Size:        821     259
 
Bme1580I G_kGCm'C

Cuts at:      0     724     737     879    1080 
   Size:        724      13     142     201

  Fragments arranged by size: 

                 724     201     142      13
 
BmgBI CAC'GTC

Cuts at:      0     762    1080 
   Size:        762     318
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      43      97     409    1080 
   Size:         43      54     312     671

  Fragments arranged by size: 

                 671     312      54      43
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     385    1080 
   Size:        385     695
 
BsaAI yAC'GTr

Cuts at:      0     732    1080 
   Size:        732     348
 
BsaBI GATnn'nnATC

Cuts at:      0     406     484    1080 
   Size:        406      78     596

  Fragments arranged by size: 

                 596     406      78
 
BsaHI Gr'CG_yC

Cuts at:      0     629    1080 
   Size:        629     451
 
BsaWI w'CCGG_w

Cuts at:      0    1013    1080 
   Size:       1013      67
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      20      50     304     334    1080 
   Size:         20      30     254      30     746

  Fragments arranged by size: 

                 746     254      30      30      20
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     703    1080 
   Size:        703     377
 
BseYI C'CCAG_C

Cuts at:      0      83     872    1080 
   Size:         83     789     208

  Fragments arranged by size: 

                 789     208      83
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     301     742    1080 
   Size:        301     441     338

  Fragments arranged by size: 

                 441     338     301
 
BsiHKAI G_wGCw'C

Cuts at:      0     170     737    1080 
   Size:        170     567     343

  Fragments arranged by size: 

                 567     343     170
 
BsmI GAATG_Cn'

Cuts at:      0     120    1080 
   Size:        120     960
 
Bsp1286I G_dGCh'C

Cuts at:      0      83     170     724     737     879    1080 
   Size:         83      87     554      13     142     201

  Fragments arranged by size: 

                 554     201     142      87      83      13
 
BspEI T'CCGG_A

Cuts at:      0    1013    1080 
   Size:       1013      67
 
BspHI T'CATG_A

Cuts at:      0     398     575    1080 
   Size:        398     177     505

  Fragments arranged by size: 

                 505     398     177
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     210     323     795     919    1080 
   Size:        210     113     472     124     161

  Fragments arranged by size: 

                 472     210     161     124     113
 
BsrDI GCAATG_nn'

Cuts at:      0     116    1080 
   Size:        116     964
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     115    1080 
   Size:        115     965
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     387     852    1080 
   Size:        387     465     228

  Fragments arranged by size: 

                 465     387     228
 
BtgI C'CryG_G

Cuts at:      0     296    1080 
   Size:        296     784
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     507    1080 
   Size:        507     573
 
BtsI GCAGTG_nn'

Cuts at:      0     148     778     941    1080 
   Size:        148     630     163     139

  Fragments arranged by size: 

                 630     163     148     139
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     538    1080 
   Size:        538     542
 
EaeI y'GGCC_r

Cuts at:      0     293     356     518     608    1080 
   Size:        293      63     162      90     472

  Fragments arranged by size: 

                 472     293     162      90      63
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      43      97     171     409    1080 
   Size:         43      54      74     238     671

  Fragments arranged by size: 

                 671     238      74      54      43
 
EcoO109I rG'GnC_Cy

Cuts at:      0     591     876    1080 
   Size:        591     285     204

  Fragments arranged by size: 

                 591     285     204
 
FspI TGC'GCA

Cuts at:      0    1030    1080 
   Size:       1030      50
 
HaeII r_GCGC'y

Cuts at:      0     209    1080 
   Size:        209     871
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      20      52    1080 
   Size:         20      32    1028

  Fragments arranged by size: 

                1028      32      20
 
HincII GTy'rAC

Cuts at:      0     531    1080 
   Size:        531     549
 
MscI TGG'CCA

Cuts at:      0     520     610    1080 
   Size:        520      90     470

  Fragments arranged by size: 

                 520     470      90
 
MslI CAynn'nnrTG

Cuts at:      0     580     691    1080 
   Size:        580     111     389

  Fragments arranged by size: 

                 580     389     111
 
NsiI A_TGCA'T

Cuts at:      0     823    1080 
   Size:        823     257
 
NspI r_CATG'y

Cuts at:      0     366     741    1080 
   Size:        366     375     339

  Fragments arranged by size: 

                 375     366     339
 
PciI A'CATG_T

Cuts at:      0     737    1080 
   Size:        737     343
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     456    1080 
   Size:        456     624
 
PspOMI G'GGCC_C

Cuts at:      0     875    1080 
   Size:        875     205
 
PstI C_TGCA'G

Cuts at:      0     346    1080 
   Size:        346     734
 
SalI G'TCGA_C

Cuts at:      0     529    1080 
   Size:        529     551
 
SexAI A'CCwGG_T

Cuts at:      0     893     962    1080 
   Size:        893      69     118

  Fragments arranged by size: 

                 893     118      69
 
SfcI C'TryA_G

Cuts at:      0     342    1080 
   Size:        342     738
 
TatI w'GTAC_w

Cuts at:      0     329    1080 
   Size:        329     751
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII     ApaI    ApaLI     AvaI     BanI
    BanII     BbsI    BfrBI Bme1580I    BmgBI     BpmI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrDI   BstAPI    BstXI
     BtgI    BtgZI     BtsI     DrdI     EaeI  Eco57MI EcoO109I     FspI
    HaeII    Hin4I   HincII     MscI     MslI     NsiI     NspI     PciI
    PflMI   PspOMI     PstI     SalI    SexAI     SfcI     TatI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AleI
     AlfI     AloI    AlwNI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI     BclI     BglI
    BglII     BlpI     BmrI     BmtI     BplI   Bpu10I    BpuEI    BsiEI
    BsiWI    BsmBI    BsrBI    BsrFI    BsrGI   BssHII    BssSI    BstBI
   BstEII    BstYI  BstZ17I   Bsu36I     ClaI    CspCI     DraI   DraIII
     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI
     FseI    FspAI  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     PacI     PasI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII    SanDI     SapI     SbfI     ScaI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI
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