Restriction map for pF1KE0802
##################################
# NO CUT ENZYMES (  163 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AflII       AgeI        AhdI        AlwNI       AocI        
Aor51HI     ApaI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvaI        
AviII       AvrII       BamHI       BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BcnI        BfaI        BfrI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaI        
BsaWI       BseAI       BseRI       BsiEI       BsiWI       
BsmBI       BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HapII       HhaI        HinP1I      HpaII       
HphI        KasI        KpnI        KspI        MaeI        
MfeI        MluI        MroI        MspI        MunI        
MvnI        NaeI        NarI        NciI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TaqI        Tsp45I      
Tth111I     TthHB8I     Van91I      XbaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AccI          877  GTCTAC               GT^[AC][GT]AC
AcsI            5  AAATTT               [GA]^AATT[CT]
AcyI          501  GGCGTC               G[GA]^CG[CT]C
Alw44I        787  GTGCAC               G^TGCAC
AlwI          900  GATCC                GGATC(4/5)
ApaLI         787  GTGCAC               G^TGCAC
ApoI            5  AAATTT               [GA]^AATT[CT]
AvaII         370  GGACC                G^G[AT]CC
BanI          907  GGTGCC               G^G[CT][GA]CC
BanII         661  GGGCTC               G[GA]GC[CT]^C
BsaHI         501  GGCGTC               G[GA]^CG[CT]C
BsgI          548  CTGCAC               GTGCAG(16/14)
BsmAI         226  GTCTC                GTCTC(1/5)
BsmI          920  GAATGC               GAATGC(1/-1)
BspLU11I      724  ACATGT               A^CATGT
BstXI         360  CCATGTCTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         899  AGATCC               [GA]^GATC[CT]
Cfr13I        370  GGACC                G^G[ACGT]CC
DdeI          398  CTGAG                C^T[ACGT]AG
DsaI          903  CCATGG               C^C[GA][CT]GG
EcoRV          87  GATATC               GAT^ATC
Hin1I         501  GGCGTC               G[GA]^CG[CT]C
HincII        586  GTTAAC               GT[CT]^[GA]AC
HindII        586  GTTAAC               GT[CT]^[GA]AC
HindIII       293  AAGCTT               A^AGCTT
HpaI          586  GTTAAC               GTT^AAC
MaeIII        613  GTTAC                ^GT[ACGT]AC
MflI          899  AGATCC               [GA]^GATC[CT]
MslI          736  CACGTCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        345  CAGCTG               C[AC]G^C[GT]G
NcoI          903  CCATGG               C^CATGG
NspI          724  ACATGT               [GA]CATG^[CT]
PleI           70  GAGTC                GAGTC(4/5)
PvuII         345  CAGCTG               CAG^CTG
Sau96I        370  GGACC                G^G[ACGT]CC
TfiI          634  GATTC                G^A[AT]TC
XcmI          200  CCATGTTGGCACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         899  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AciI          373  CCGC                 CCGC(-3/-1)
              792  CCGC                
AfaI          186  GTAC                 GT^AC
              455  GTAC                
AflIII        724  ACATGT               A^C[GA][CT]GT
              735  ACACGT              
AspHI          76  GAGCAC               G[AT]GC[AT]^C
              787  GTGCAC              
BbvI          352  GCTGC                GCAGC(8/12)
              819  GCTGC               
BglI          199  GCCATGTTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              826  GCCATGATGGC         
BpmI           67  CTGGAG               CTGGAG(16/14)
              695  CTGGAG              
BsaBI         108  GATATACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              645  GATTGTGATC          
BsiHKAI        76  GAGCAC               G[AT]GC[AT]^C
              787  GTGCAC              
BsmFI          94  GTCCC                GGGAC(10/14)
              810  GTCCC               
BsrI          210  ACTGG                ACTGG(1/-1)
              694  ACTGG               
BssKI          61  CCAGG                ^CC[ACGT]GG
              426  CCAGG               
BstNI          61  CCAGG                CC^[AT]GG
              426  CCAGG               
DpnII         651  GATC                 ^GATC
              900  GATC                
EarI          190  CTCTTC               CTCTTC(1/4)
              847  CTCTTC              
EcoRII         61  CCAGG                ^CC[AT]GG
              426  CCAGG               
Fnu4HI        352  GCTGC                GC^[ACGT]GC
              819  GCTGC               
HgaI          938  GACGC                GACGC(5/10)
              502  GCGTC               
HinfI          70  GAGTC                G^A[ACGT]TC
              634  GATTC               
ItaI          352  GCTGC                GC^[ACGT]GC
              819  GCTGC               
Ksp632I       190  CTCTTC               CTCTTC(1/4)
              847  CTCTTC              
MaeII         149  ACGT                 A^CGT
              737  ACGT                
MamI          108  GATATACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              645  GATTGTGATC          
MboI          651  GATC                 ^GATC
              900  GATC                
MseI          587  TTAA                 T^TAA
              887  TTAA                
MvaI           61  CCAGG                CC^[AT]GG
              426  CCAGG               
NdeII         651  GATC                 ^GATC
              900  GATC                
RsaI          186  GTAC                 GT^AC
              455  GTAC                
Sau3AI        651  GATC                 ^GATC
              900  GATC                
ScrFI          61  CCAGG                CC^[ACGT]GG
              426  CCAGG               
Tru9I         587  TTAA                 T^TAA
              887  TTAA                
TseI          352  GCTGC                G^C[AT]GC
              819  GCTGC               
Tsp509I         6  AATT                 ^AATT
              282  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   24 enzymes) #
#########################################

AluI        BalI        BmyI        BsaJI       BsiYI       
BslI        Bsp1286I    BstF5I      Cac8I       EaeI        
EcoT14I     FokI        HaeIII      MboII       MluNI       
MnlI        MscI        MwoI        NlaIII      NlaIV       
SfaNI       SfcI        StyI        TspRI       
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