Restriction map for pF1KE0790
##################################
# NO CUT ENZYMES (  163 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AvaI        
AviII       AvrII       BamHI       BanI        BbeI        
BbrPI       BbvCI       BcgI        BciVI       BclI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsaWI       
BseAI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstYI       BstZ17I     CelII       Cfr10I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        DsaI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoNI       EcoO109I    EcoO65I     
EcoRV       EcoT14I     FauI        FbaI        FseI        
FspI        HaeII       Hin1I       HincII      HindII      
HpaI        KasI        KpnI        KspI        MamI        
MfeI        MflI        MluI        MroI        MunI        
MvnI        MwoI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PaeR7I      PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AocI           58  CCTCAGG              CC^T[ACGT]AGG
AspI         1216  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaII         123  GGACC                G^G[AT]CC
BalI           86  TGGCCA               TGG^CCA
BanII         241  GAGCCC               G[GA]GC[CT]^C
BbvI          408  GCAGC                GCAGC(8/12)
BcnI         1153  CCGGG                CC^[GC]GG
BfaI          506  CTAG                 C^TAG
BmyI          241  GAGCCC               G[AGT]GC[ACT]^C
BsaI         1275  GAGACC               GGTCTC(1/5)
BsiYI          20  CCCAGAAGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           20  CCCAGAAGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI        1275  GAGAC                GTCTC(1/5)
Bsp1286I      241  GAGCCC               G[AGT]GC[ACT]^C
BsrDI         341  GCAATG               GCAATG(2/0)
BstF5I         47  GGATG                GGATG(2/0)
Bsu36I         58  CCTCAGG              CC^T[ACGT]AGG
Cac8I         649  GCAAGC               GC[ACGT]^[ACGT]GC
CfoI         1230  GCGC                 GCG^C
DpnII         544  GATC                 ^GATC
EaeI           86  TGGCCA               [CT]^GGCC[GA]
Eco81I         58  CCTCAGG              CC^T[ACGT]AGG
EcoT22I      1292  ATGCAT               ATGCA^T
Fnu4HI        408  GCAGC                GC^[ACGT]GC
FokI           47  GGATG                GGATG(9/13)
HhaI         1230  GCGC                 GCG^C
HinP1I       1230  GCGC                 G^CGC
HindIII       884  AAGCTT               A^AGCTT
ItaI          408  GCAGC                GC^[ACGT]GC
MaeI          506  CTAG                 C^TAG
MboI          544  GATC                 ^GATC
MluNI          86  TGGCCA               TGG^CCA
MscI           86  TGGCCA               TGG^CCA
MslI           78  CATGGACGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        409  CAGCTG               C[AC]G^C[GT]G
NciI         1153  CCGGG                CC^[GC]GG
NdeII         544  GATC                 ^GATC
NsiI         1292  ATGCAT               ATGCA^T
NspI         1294  GCATGT               [GA]CATG^[CT]
PflFI        1216  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PvuII         409  CAGCTG               CAG^CTG
Sau3AI        544  GATC                 ^GATC
SexAI         176  ACCTGGT              A^CC[AT]GGT
SfaNI        1083  GCATC                GCATC(5/9)
SfcI          168  CTACAG               C^T[GA][CT]AG
SmlI          385  CTTGAG               C^T[CT][GA]AG
TaqI           92  TCGA                 T^CGA
TseI          408  GCAGC                G^C[AT]GC
TspRI        1364  CACTG                CA[GC]TG[ACGT][ACGT]^
Tth111I      1216  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I        92  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AciI          680  CCGC                 CCGC(-3/-1)
             1018  GCGG                
BsaJI         119  CCCTGG               C^C[ACGT][ACGT]GG
              126  CCCTGG              
BseRI         106  GAGGAG               GAGGAG(10/8)
              321  GAGGAG              
HapII         422  CCGG                 C^CGG
             1153  CCGG                
HgaI           65  GACGC                GACGC(5/10)
             1149  GCGTC               
HpaII         422  CCGG                 C^CGG
             1153  CCGG                
HphI          628  GGTGA                GGTGA(8/7)
              484  TCACC               
MaeII          83  ACGT                 A^CGT
              152  ACGT                
MspI          422  CCGG                 C^CGG
             1153  CCGG                
NlaIV         123  GGACCC               GG[ACGT]^[ACGT]CC
              173  GGAACC              
SapI         1133  GAAGAGC              GCTCTTC(1/4)
               52  GCTCTTC             
TfiI          191  GAATC                G^A[AT]TC
              249  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (   32 enzymes) #
#########################################

AcsI        AfaI        AluI        ApoI        BbsI        
BpmI        BpuAI       BsmFI       BsrI        BssKI       
BstNI       Cfr13I      DdeI        EarI        EcoRI       
EcoRII      HaeIII      HinfI       Ksp632I     MaeIII      
MboII       MnlI        MseI        MvaI        NlaIII      
PleI        RsaI        Sau96I      ScrFI       Tru9I       
Tsp45I      Tsp509I     
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