Restriction map for
pF1KE0790
(Circular) (Six-base) MAPSORT of: pF1KE0790.seq Check: 8873 from: 1 to: 4499
pF1KE0790 4499 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AccI GT'mk_AC
Cuts at: 1537 1537
Size: 4499
Acc65I G'GTAC_C
Cuts at: 1515 1515
Size: 4499
AclI AA'CG_TT
Cuts at: 4339 4339
Size: 4499
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 813 1065 2324 2756 3503 3771 813
Size: 252 1259 432 747 268 1541
Fragments arranged by size:
1541 1259 747 432 268 252
AflIII A'CryG_T
Cuts at: 1851 2955 3932 1851
Size: 1104 977 2418
Fragments arranged by size:
2418 1104 977
AgeI A'CCGG_T
Cuts at: 2887 2887
Size: 4499
AlwNI CAG_nnn'CTG
Cuts at: 1804 3371 1804
Size: 1567 2932
ApaLI G'TGCA_C
Cuts at: 3269 3269
Size: 4499
ApoI r'AATT_y
Cuts at: 202 539 605 863 947 1090 1367 1451 1467
Size: 337 66 258 84 143 277 84 16
Cuts at: 1467 1503 3852 4108 202
Size: 36 2349 256 593
Fragments arranged by size:
2349 593 337 277 258 256 143 84
84 66 36 16
AseI AT'TA_AT
Cuts at: 20 20
Size: 4499
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4499
AvaI C'yCGr_G
Cuts at: 1519 1519
Size: 4499
BamHI G'GATC_C
Cuts at: 1524 1524
Size: 4499
BanI G'GyrC_C
Cuts at: 1515 1929 2177 2212 1515
Size: 414 248 35 3802
Fragments arranged by size:
3802 414 248 35
BanII G_rGCy'C
Cuts at: 353 1513 2543 353
Size: 1160 1030 2309
Fragments arranged by size:
2309 1160 1030
BbeI G_GCGC'C
Cuts at: 1933 2181 1933
Size: 248 4251
BbsI GAAGACnn'nnnn_
Cuts at: 298 819 1407 298
Size: 521 588 3390
Fragments arranged by size:
3390 588 521
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4465
BciVI GTATCCnnnnn_n'
Cuts at: 2397 3158 4499 2397
Size: 761 1341 2397
Fragments arranged by size:
2397 1341 761
BclI T'GATC_A
Cuts at: 2019 2019
Size: 4499
BfrBI ATG'CAT
Cuts at: 1402 1402
Size: 4499
BglII A'GATC_T
Cuts at: 2014 4051 2014
Size: 2037 2462
BlpI GC'TnA_GC
Cuts at: 1611 1611
Size: 4499
Bme1580I G_kGCm'C
Cuts at: 2124 2217 3273 2124
Size: 93 1056 3350
Fragments arranged by size:
3350 1056 93
BmgBI CAC'GTC
Cuts at: 192 261 192
Size: 69 4430
BmrI ACTGGGnnnn_n'
Cuts at: 1711 1792 2127 4271 1711
Size: 81 335 2144 1939
Fragments arranged by size:
2144 1939 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2866 2866
Size: 4499
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 977 1061 1313 1397 1481 977
Size: 84 252 84 84 3995
Fragments arranged by size:
3995 252 84 84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 514 1675 2554 3046 3344 514
Size: 1161 879 492 298 1669
Fragments arranged by size:
1669 1161 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1377 4203 1377
Size: 2826 1673
BsaAI yAC'GTr
Cuts at: 1852 1852
Size: 4499
BsaBI GATnn'nnATC
Cuts at: 6 2013 4050 6
Size: 2007 2037 455
Fragments arranged by size:
2037 2007 455
BsaHI Gr'CG_yC
Cuts at: 1930 2178 2880 4376 1930
Size: 248 702 1496 2053
Fragments arranged by size:
2053 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1688 2209 2887 3161 3308 3719 1688
Size: 521 678 274 147 411 2468
Fragments arranged by size:
2468 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 420 450 420
Size: 30 4469
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 229 444 229
Size: 215 4284
BseYI C'CCAG_C
Cuts at: 1924 2682 3259 3755 1924
Size: 758 577 496 2668
Fragments arranged by size:
2668 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2041 2087 3295 104
Size: 1937 46 1208 1308
Fragments arranged by size:
1937 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1513 2291 2481 3273 1513
Size: 778 190 792 2739
Fragments arranged by size:
2739 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 353 1513 2124 2217 2291 2481 2543 3273 353
Size: 1160 611 93 74 190 62 730 1579
Fragments arranged by size:
1579 1160 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3719 3719
Size: 4499
BspHI T'CATG_A
Cuts at: 3675 3675
Size: 4499
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1549 1763 2065 2446 1549
Size: 214 302 381 3602
Fragments arranged by size:
3602 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2791 2845 4329 4494 50
Size: 2741 54 1484 165 55
Fragments arranged by size:
2741 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 456 2411 456
Size: 1955 2544
BsrFI r'CCGG_y
Cuts at: 2497 2678 2887 2497
Size: 181 209 4109
Fragments arranged by size:
4109 209 181
BssSI C'ACGA_G
Cuts at: 2770 3128 2770
Size: 358 4141
BstAPI GCAn_nnn'nTGC
Cuts at: 1876 1876
Size: 4499
BstBI TT'CG_AA
Cuts at: 2860 2860
Size: 4499
BstYI r'GATC_y
Cuts at: 1 1524 1991 2014 2349 2595 3607 3693 3705
Size: 1523 467 23 335 246 1012 86 12
Cuts at: 3705 4051 1
Size: 346 449
Fragments arranged by size:
1523 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 167 167
Size: 4499
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2449 2630 2793 2449
Size: 181 163 4155
Fragments arranged by size:
4155 181 163
BtsI GCAGTG_nn'
Cuts at: 1471 1742 1471
Size: 271 4228
DraI TTT'AAA
Cuts at: 1498 1498
Size: 4499
DrdI GACnn_nn'nnGTC
Cuts at: 2205 3063 2205
Size: 858 3641
EaeI y'GGCC_r
Cuts at: 194 2084 2258 2649 2676 4021 194
Size: 1890 174 391 27 1345 672
Fragments arranged by size:
1890 1345 672 391 174 27
EagI C'GGCC_G
Cuts at: 2084 2084
Size: 4499
EarI CTCTTCn'nnn_
Cuts at: 127 167 677 1236 2522 2732 127
Size: 40 510 559 1286 210 1894
Fragments arranged by size:
1894 1286 559 510 210 40
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1141 2707 3017 3163 4309 1141
Size: 1566 310 146 1146 1331
Fragments arranged by size:
1566 1331 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1511 1511
Size: 4499
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 813 977 1061 1065 1313 1397 1481 2324 2756
Size: 164 84 4 248 84 84 843 432
Cuts at: 2756 3503 3771 813
Size: 747 268 1541
Fragments arranged by size:
1541 843 747 432 268 248 164 84
84 84 4
EcoO109I rG'GnC_Cy
Cuts at: 1638 1638
Size: 4499
EcoRI G'AATT_C
Cuts at: 202 863 947 1367 1467 1503 202
Size: 661 84 420 100 36 3198
Fragments arranged by size:
3198 661 420 100 84 36
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2518 2518
Size: 4499
FspI TGC'GCA
Cuts at: 2280 2280
Size: 4499
HaeII r_GCGC'y
Cuts at: 1933 2181 3203 4182 1933
Size: 248 1022 979 2250
Fragments arranged by size:
2250 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 208 240 2341 2371 2373 2403 208
Size: 32 2101 30 2 30 2304
Fragments arranged by size:
2304 2101 32 30 30 2
HincII GTy'rAC
Cuts at: 1538 1538
Size: 4499
HindIII A'AGCT_T
Cuts at: 992 992
Size: 4499
KasI G'GCGC_C
Cuts at: 1929 2177 1929
Size: 248 4251
KpnI G_GTAC'C
Cuts at: 1519 1519
Size: 4499
MluI A'CGCG_T
Cuts at: 3932 3932
Size: 4499
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 248 3170 3354 248
Size: 2922 184 1393
Fragments arranged by size:
2922 1393 184
MscI TGG'CCA
Cuts at: 196 2260 196
Size: 2064 2435
MslI CAynn'nnrTG
Cuts at: 190 2488 2615 190
Size: 2298 127 2074
Fragments arranged by size:
2298 2074 127
MspA1I CmG'CkG
Cuts at: 519 1610 1924 2284 3297 3542 519
Size: 1091 314 360 1013 245 1476
Fragments arranged by size:
1476 1091 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2680 2680
Size: 4499
NarI GG'CG_CC
Cuts at: 1930 2178 1930
Size: 248 4251
NgoMIV G'CCGG_C
Cuts at: 2678 2678
Size: 4499
NsiI A_TGCA'T
Cuts at: 1404 1404
Size: 4499
NspI r_CATG'y
Cuts at: 1406 1552 2052 2959 1406
Size: 146 500 907 2946
Fragments arranged by size:
2946 907 500 146
PciI A'CATG_T
Cuts at: 2955 2955
Size: 4499
PmeI GTTT'AAAC
Cuts at: 1498 1498
Size: 4499
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3638 3670 3638
Size: 32 4467
PstI C_TGCA'G
Cuts at: 1546 2231 1546
Size: 685 3814
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4499
PvuII CAG'CTG
Cuts at: 519 1924 2284 519
Size: 1405 360 2734
Fragments arranged by size:
2734 1405 360
RsrII CG'GwC_CG
Cuts at: 2694 2694
Size: 4499
SacI G_AGCT'C
Cuts at: 1513 1513
Size: 4499
SalI G'TCGA_C
Cuts at: 1536 1536
Size: 4499
SapI GCTCTTCn'nnn_
Cuts at: 167 1236 2522 2732 167
Size: 1069 1286 210 1934
Fragments arranged by size:
1934 1286 1069 210
SbfI CC_TGCA'GG
Cuts at: 1546 1546
Size: 4499
ScaI AGT'ACT
Cuts at: 4016 4016
Size: 4499
SexAI A'CCwGG_T
Cuts at: 284 284
Size: 4499
SfcI C'TryA_G
Cuts at: 33 276 1542 2227 3220 3411 33
Size: 243 1266 685 993 191 1121
Fragments arranged by size:
1266 1121 993 685 243 191
SfoI GGC'GCC
Cuts at: 1931 2179 1931
Size: 248 4251
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4499
SmaI CCC'GGG
Cuts at: 1521 1521
Size: 4499
SmlI C'TyrA_G
Cuts at: 493 1654 2569 3061 3323 493
Size: 1161 915 492 262 1669
Fragments arranged by size:
1669 1161 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4499
SphI G_CATG'C
Cuts at: 1552 2052 1552
Size: 500 3999
StyI C'CwwG_G
Cuts at: 1633 1987 1633
Size: 354 4145
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2082 2215 2674 2883 2082
Size: 133 459 209 3698
Fragments arranged by size:
3698 459 209 133
TatI w'GTAC_w
Cuts at: 808 1207 4014 808
Size: 399 2807 1293
Fragments arranged by size:
2807 1293 399
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 420 452 4016 4048 420
Size: 32 3564 32 871
Fragments arranged by size:
3564 871 32 32
Tth111I GACn'n_nGTC
Cuts at: 1327 2296 1327
Size: 969 3530
XbaI T'CTAG_A
Cuts at: 1530 1530
Size: 4499
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1994 1994
Size: 4499
XmaI C'CCGG_G
Cuts at: 1519 1519
Size: 4499
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NsiI
NspI PciI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BglI BmtI Bpu10I
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HpaI MfeI NcoI NdeI NheI NotI NruI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI