Restriction map for pF1KE0790
 (Linear) (Six-base) MAPSORT of: KIEE0790.seq  Check: 4302  from: 1  to: 1386



KIEE0790.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:17  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     705     957    1386 
   Size:        705     252     429

  Fragments arranged by size: 

                 705     429     252
 
ApoI r'AATT_y

Cuts at:      0      94     431     497     755     839     982    1259    1343 
   Size:         94     337      66     258      84     143     277      84

Cuts at:   1343    1359    1386 
   Size:         16      27

  Fragments arranged by size: 

                 337     277     258     143      94      84      84      66
                  27      16
 
BanII G_rGCy'C

Cuts at:      0     245    1386 
   Size:        245    1141
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     190     711    1299    1386 
   Size:        190     521     588      87

  Fragments arranged by size: 

                 588     521     190      87
 
BfrBI ATG'CAT

Cuts at:      0    1294    1386 
   Size:       1294      92
 
BmgBI CAC'GTC

Cuts at:      0      84     153    1386 
   Size:         84      69    1233

  Fragments arranged by size: 

                1233      84      69
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     869     953    1205    1289    1373    1386 
   Size:        869      84     252      84      84      13

  Fragments arranged by size: 

                 869     252      84      84      84      13
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     406    1386 
   Size:        406     980
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1269    1386 
   Size:       1269     117
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     312     342    1386 
   Size:        312      30    1044

  Fragments arranged by size: 

                1044     312      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     121     336    1386 
   Size:        121     215    1050

  Fragments arranged by size: 

                1050     215     121
 
Bsp1286I G_dGCh'C

Cuts at:      0     245    1386 
   Size:        245    1141
 
BsrDI GCAATG_nn'

Cuts at:      0     348    1386 
   Size:        348    1038
 
Bsu36I CC'TnA_GG

Cuts at:      0      59    1386 
   Size:         59    1327
 
BtsI GCAGTG_nn'

Cuts at:      0    1363    1386 
   Size:       1363      23
 
EaeI y'GGCC_r

Cuts at:      0      86    1386 
   Size:         86    1300
 
EarI CTCTTCn'nnn_

Cuts at:      0      19      59     569    1128    1386 
   Size:         19      40     510     559     258

  Fragments arranged by size: 

                 559     510     258      40      19
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1033    1386 
   Size:       1033     353
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     705     869     953     957    1205    1289    1373    1386 
   Size:        705     164      84       4     248      84      84      13

  Fragments arranged by size: 

                 705     248     164      84      84      84      13       4
            
 
EcoRI G'AATT_C

Cuts at:      0      94     755     839    1259    1359    1386 
   Size:         94     661      84     420     100      27

  Fragments arranged by size: 

                 661     420     100      94      84      27
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     100     132    1386 
   Size:        100      32    1254

  Fragments arranged by size: 

                1254     100      32
 
HindIII A'AGCT_T

Cuts at:      0     884    1386 
   Size:        884     502
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     140    1386 
   Size:        140    1246
 
MscI TGG'CCA

Cuts at:      0      88    1386 
   Size:         88    1298
 
MslI CAynn'nnrTG

Cuts at:      0      82    1386 
   Size:         82    1304
 
MspA1I CmG'CkG

Cuts at:      0     411    1386 
   Size:        411     975
 
NsiI A_TGCA'T

Cuts at:      0    1296    1386 
   Size:       1296      90
 
NspI r_CATG'y

Cuts at:      0    1298    1386 
   Size:       1298      88
 
PvuII CAG'CTG

Cuts at:      0     411    1386 
   Size:        411     975
 
SapI GCTCTTCn'nnn_

Cuts at:      0      59    1128    1386 
   Size:         59    1069     258

  Fragments arranged by size: 

                1069     258      59
 
SexAI A'CCwGG_T

Cuts at:      0     176    1386 
   Size:        176    1210
 
SfcI C'TryA_G

Cuts at:      0     168    1386 
   Size:        168    1218
 
SmlI C'TyrA_G

Cuts at:      0     385    1386 
   Size:        385    1001
 
TatI w'GTAC_w

Cuts at:      0     700    1099    1386 
   Size:        700     399     287

  Fragments arranged by size: 

                 700     399     287
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     312     344    1386 
   Size:        312      32    1042

  Fragments arranged by size: 

                1042     312      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1219    1386 
   Size:       1219     167
 

 Enzymes that do cut:

     AcuI     ApoI    BanII     BbsI    BfrBI    BmgBI     BpmI    BpuEI
     BsaI    BsaXI    BseRI Bsp1286I    BsrDI   Bsu36I     BtsI     EaeI
     EarI     EciI  Eco57MI    EcoRI    Hin4I  HindIII     MmeI     MscI
     MslI   MspA1I     NsiI     NspI    PvuII     SapI    SexAI     SfcI
     SmlI     TatI     TstI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI    BbvCI     BcgI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I     BmrI     BmtI     BplI   Bpu10I    BsaAI    BsaBI    BsaHI
    BsaWI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I     BtgI    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI  EcoICRI    EcoNI
 EcoO109I    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
     HpaI     KasI     KpnI     MfeI     MluI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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