Restriction map for pF1KE0788
##################################
# NO CUT ENZYMES (  112 enzymes) #
##################################

Acc65I      AccI        AclI        AcsI        AflII       
AflIII      AgeI        Alw44I      AocI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AviII       AvrII       BamHI       BbeI        
BbrPI       BbvCI       BcgI        BciVI       BclI        
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsiWI       Bsp1407I    BspDI       
BspLU11I    BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstXI       
BstYI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DraI        DraIII      Eco81I      EcoO65I     
EcoRI       EcoT22I     FbaI        FseI        FspI        
HindIII     HpaI        KasI        KpnI        MamI        
MfeI        MflI        MluI        MunI        NarI        
NdeI        NheI        NruI        NsiI        NspV        
PacI        PaeR7I      PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
PvuII       RsrII       SalI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TfiI        
Tsp509I     XbaI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AccIII        416  TCCGGA               T^CCGGA
AfaI          690  GTAC                 GT^AC
AlwI           80  GGATC                GGATC(4/5)
ApaI          633  GGGCCC               GGGCC^C
AspI         1200  GACTGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI          140  TGGCCA               TGG^CCA
BfaI         1217  CTAG                 C^TAG
BglI          844  GCCTACATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI         1101  GAGACC               GGTCTC(1/5)
BsaWI         416  TCCGGA               [AT]^CCGG[AT]
BseAI         416  TCCGGA               T^CCGGA
BseRI         472  GAGGAG               GAGGAG(10/8)
BsgI          538  GTGCAG               GTGCAG(16/14)
BsiEI         795  CGGCCG               CG[GA][CT]^CG
BsmBI        1150  GAGACG               CGTCTC(1/5)
BsmI          650  GCATTC               GAATGC(1/-1)
BspEI         416  TCCGGA               T^CCGGA
BspHI         296  TCATGA               T^CATGA
BsrDI         435  CATTGC               GCAATG(2/0)
BsrI         1059  CCAGT                ACTGG(1/-1)
DrdI          982  GACTTTGAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI          795  CGGCCG               C^GGCCG
EclXI         795  CGGCCG               C^GGCCG
Eco52I        795  CGGCCG               C^GGCCG
EcoNI        1247  CCTTCTTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV          97  GATATC               GAT^ATC
EcoT14I       584  CCATGG               C^C[AT][AT]GG
HincII        234  GTCAAC               GT[CT]^[GA]AC
HindII        234  GTCAAC               GT[CT]^[GA]AC
KspI         1210  CCGCGG               CCGC^GG
MaeI         1217  CTAG                 C^TAG
MaeIII       1206  GTCAC                ^GT[ACGT]AC
MluNI         140  TGGCCA               TGG^CCA
MroI          416  TCCGGA               T^CCGGA
MscI          140  TGGCCA               TGG^CCA
MseI          443  TTAA                 T^TAA
NaeI          832  GCCGGC               GCC^GGC
NcoI          584  CCATGG               C^CATGG
NgoMIV        832  GCCGGC               G^CCGGC
NotI          794  GCGGCCGC             GC^GGCCGC
NspI          218  ACATGC               [GA]CATG^[CT]
PflFI        1200  GACTGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         216  CCACATGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         989  AGGTCCT              [GA]G^G[AT]CC[CT]
RcaI          296  TCATGA               T^CATGA
RsaI          690  GTAC                 GT^AC
SacII        1210  CCGCGG               CCGC^GG
SmlI          616  CTCAAG               C^T[CT][GA]AG
StyI          584  CCATGG               C^C[AT][AT]GG
TaqI           83  TCGA                 T^CGA
Tru9I         443  TTAA                 T^TAA
Tsp45I       1206  GTCAC                ^GT[GC]AC
Tth111I      1200  GACTGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I        83  TCGA                 T^CGA
Van91I        216  CCACATGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AatII         571  GACGTC               GACGT^C
              727  GACGTC              
AhdI         1054  GACTTCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1165  GACGTGGCGTC         
AlwNI          22  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              280  CAGAAGCTG           
Aor51HI       332  AGCGCT               AGC^GCT
             1142  AGCGCT              
AspEI        1054  GACTTCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1165  GACGTGGCGTC         
AspHI         177  GAGCTC               G[AT]GC[AT]^C
              589  GAGCTC              
AvaII         495  GGACC                G^G[AT]CC
              990  GGTCC               
BpmI          358  CTGGAG               CTGGAG(16/14)
             1156  CTGGAG              
BsiHKAI       177  GAGCTC               G[AT]GC[AT]^C
              589  GAGCTC              
BsmAI        1101  GAGAC                GTCTC(1/5)
             1150  GAGAC               
BsrBI         159  CCGCTC               CCGCTC(-3/-3)
              757  GAGCGG              
BsrFI         424  ACCGGC               [GA]^CCGG[CT]
              832  GCCGGC              
Cfr10I        424  ACCGGC               [GA]^CCGG[CT]
              832  GCCGGC              
DsaI          584  CCATGG               C^C[GA][CT]GG
             1210  CCGCGG              
Eam1105I     1054  GACTTCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1165  GACGTGGCGTC         
Eco47III      332  AGCGCT               AGC^GCT
             1142  AGCGCT              
HinfI         808  GACTC                G^A[ACGT]TC
             1204  GAGTC               
MslI          486  CATCCTCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              579  CATCGCCATG          
PleI          808  GACTC                GAGTC(4/5)
             1204  GAGTC               
PstI          127  CTGCAG               CTGCA^G
              286  CTGCAG              
SacI          177  GAGCTC               GAGCT^C
              589  GAGCTC              
SapI          513  GAAGAGC              GCTCTTC(1/4)
             1012  GAAGAGC             
TspRI         541  CAGTG                CA[GC]TG[ACGT][ACGT]^
              668  CAGTG               
XcmI          584  CCATGGAGCTCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              740  CCAACATCCTGCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   67 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        AvaI        
BanI        BanII       BbsI        BbvI        BcnI        
BmyI        BpuAI       BsaHI       BsaJI       BsiYI       
BslI        BsmFI       BsoBI       Bsp1286I    BspMI       
BssKI       BstF5I      BstNI       BstUI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
EaeI        EarI        EcoO109I    EcoRII      FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HpaII       
HphI        ItaI        Ksp632I     MaeII       MboI        
MboII       MnlI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        TseI        
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