Restriction map for pF1KE0788
 (Linear) (Six-base) MAPSORT of: KIEE0788.seq  Check: 4647  from: 1  to: 1257



KIEE0788.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:17  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     397     610    1250    1257 
   Size:        397     213     640       7

  Fragments arranged by size: 

                 640     397     213       7
 
AatII G_ACGT'C

Cuts at:      0     575     731    1257 
   Size:        575     156     526

  Fragments arranged by size: 

                 575     526     156
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70     532     718    1236    1257 
   Size:         70     462     186     518      21

  Fragments arranged by size: 

                 518     462     186      70      21
 
AfeI AGC'GCT

Cuts at:      0     334    1144    1257 
   Size:        334     810     113

  Fragments arranged by size: 

                 810     334     113
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1059    1170    1257 
   Size:       1059     111      87

  Fragments arranged by size: 

                1059     111      87
 
AlwNI CAG_nnn'CTG

Cuts at:      0      27     285    1257 
   Size:         27     258     972

  Fragments arranged by size: 

                 972     258      27
 
ApaI G_GGCC'C

Cuts at:      0     637    1257 
   Size:        637     620
 
AvaI C'yCGr_G

Cuts at:      0     643     856    1047    1257 
   Size:        643     213     191     210

  Fragments arranged by size: 

                 643     213     210     191
 
BanI G'GyrC_C

Cuts at:      0     385     598     852    1257 
   Size:        385     213     254     405

  Fragments arranged by size: 

                 405     385     254     213
 
BanII G_rGCy'C

Cuts at:      0     181     230     593     637    1019    1257 
   Size:        181      49     363      44     382     238

  Fragments arranged by size: 

                 382     363     238     181      49      44
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     427     567    1204    1257 
   Size:        427     140     637      53

  Fragments arranged by size: 

                 637     427     140      53
 
BglI GCCn_nnn'nGGC

Cuts at:      0     850    1257 
   Size:        850     407
 
Bme1580I G_kGCm'C

Cuts at:      0     601     637    1257 
   Size:        601      36     620

  Fragments arranged by size: 

                 620     601      36
 
BmgBI CAC'GTC

Cuts at:      0     432    1167    1257 
   Size:        432     735      90

  Fragments arranged by size: 

                 735     432      90
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     379    1177    1257 
   Size:        379     798      80

  Fragments arranged by size: 

                 798     379      80
 
Bpu10I CC'TnA_GC

Cuts at:      0    1228    1257 
   Size:       1228      29
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     601    1257 
   Size:        601     656
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1095    1257 
   Size:       1095     162
 
BsaHI Gr'CG_yC

Cuts at:      0       7     572     728    1171    1223    1257 
   Size:          7     565     156     443      52      34

  Fragments arranged by size: 

                 565     443     156      52      34       7
 
BsaWI w'CCGG_w

Cuts at:      0     416    1257 
   Size:        416     841
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     487    1257 
   Size:        487     770
 
BseYI C'CCAG_C

Cuts at:      0     107     222    1257 
   Size:        107     115    1035

  Fragments arranged by size: 

                1035     115     107
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     559    1257 
   Size:        559     698
 
BsiEI CG_ry'CG

Cuts at:      0     798    1257 
   Size:        798     459
 
BsiHKAI G_wGCw'C

Cuts at:      0     181     593    1257 
   Size:        181     412     664

  Fragments arranged by size: 

                 664     412     181
 
BsmI GAATG_Cn'

Cuts at:      0     650    1257 
   Size:        650     607
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1144    1257 
   Size:       1144     113
 
Bsp1286I G_dGCh'C

Cuts at:      0     181     230     593     601     637    1019    1257 
   Size:        181      49     363       8      36     382     238

  Fragments arranged by size: 

                 382     363     238     181      49      36       8
 
BspEI T'CCGG_A

Cuts at:      0     416    1257 
   Size:        416     841
 
BspHI T'CATG_A

Cuts at:      0     296    1257 
   Size:        296     961
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     397     610    1250    1257 
   Size:        397     213     640       7

  Fragments arranged by size: 

                 640     397     213       7
 
BsrBI CCG'CTC

Cuts at:      0     161     759    1257 
   Size:        161     598     498

  Fragments arranged by size: 

                 598     498     161
 
BsrDI GCAATG_nn'

Cuts at:      0     434    1257 
   Size:        434     823
 
BsrFI r'CCGG_y

Cuts at:      0     424     832    1257 
   Size:        424     408     425

  Fragments arranged by size: 

                 425     424     408
 
BtgI C'CryG_G

Cuts at:      0     584    1210    1257 
   Size:        584     626      47

  Fragments arranged by size: 

                 626     584      47
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     564    1257 
   Size:        564     693
 
BtsI GCAGTG_nn'

Cuts at:      0     547    1257 
   Size:        547     710
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     988    1257 
   Size:        988     269
 
EaeI y'GGCC_r

Cuts at:      0     140     393     426     795    1257 
   Size:        140     253      33     369     462

  Fragments arranged by size: 

                 462     369     253     140      33
 
EagI C'GGCC_G

Cuts at:      0     795    1257 
   Size:        795     462
 
EarI CTCTTCn'nnn_

Cuts at:      0     508    1007    1093    1257 
   Size:        508     499      86     164

  Fragments arranged by size: 

                 508     499     164      86
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      90    1257 
   Size:         90    1167
 
EcoICRI GAG'CTC

Cuts at:      0     179     591    1257 
   Size:        179     412     666

  Fragments arranged by size: 

                 666     412     179
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70     379     532     718    1177    1236    1257 
   Size:         70     309     153     186     459      59      21

  Fragments arranged by size: 

                 459     309     186     153      70      59      21
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1251    1257 
   Size:       1251       6
 
EcoO109I rG'GnC_Cy

Cuts at:      0     633     634     990    1257 
   Size:        633       1     356     267

  Fragments arranged by size: 

                 633     356     267       1
 
EcoRV GAT'ATC

Cuts at:      0      99    1257 
   Size:         99    1158
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     429     999    1096    1257 
   Size:        429     570      97     161

  Fragments arranged by size: 

                 570     429     161      97
 
HaeII r_GCGC'y

Cuts at:      0     336     459     688     833    1146    1257 
   Size:        336     123     229     145     313     111

  Fragments arranged by size: 

                 336     313     229     145     123     111
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1045    1077    1156    1188    1257 
   Size:       1045      32      79      32      69

  Fragments arranged by size: 

                1045      79      69      32      32
 
HincII GTy'rAC

Cuts at:      0     236    1257 
   Size:        236    1021
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     764    1257 
   Size:        764     493
 
MscI TGG'CCA

Cuts at:      0     142    1257 
   Size:        142    1115
 
MslI CAynn'nnrTG

Cuts at:      0     490     583    1257 
   Size:        490      93     674

  Fragments arranged by size: 

                 674     490      93
 
MspA1I CmG'CkG

Cuts at:      0     111     138     383     794    1212    1257 
   Size:        111      27     245     411     418      45

  Fragments arranged by size: 

                 418     411     245     111      45      27
 
NaeI GCC'GGC

Cuts at:      0     834    1257 
   Size:        834     423
 
NcoI C'CATG_G

Cuts at:      0     584    1257 
   Size:        584     673
 
NgoMIV G'CCGG_C

Cuts at:      0     832    1257 
   Size:        832     425
 
NotI GC'GGCC_GC

Cuts at:      0     795    1257 
   Size:        795     462
 
NspI r_CATG'y

Cuts at:      0     222    1257 
   Size:        222    1035
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     222    1257 
   Size:        222    1035
 
PfoI T'CCnGG_A

Cuts at:      0     460    1257 
   Size:        460     797
 
PpuMI rG'GwC_Cy

Cuts at:      0     990    1257 
   Size:        990     267
 
PspOMI G'GGCC_C

Cuts at:      0     633    1257 
   Size:        633     624
 
PstI C_TGCA'G

Cuts at:      0     131     290    1257 
   Size:        131     159     967

  Fragments arranged by size: 

                 967     159     131
 
SacI G_AGCT'C

Cuts at:      0     181     593    1257 
   Size:        181     412     664

  Fragments arranged by size: 

                 664     412     181
 
SacII CC_GC'GG

Cuts at:      0    1213    1257 
   Size:       1213      44
 
SapI GCTCTTCn'nnn_

Cuts at:      0     508    1007    1257 
   Size:        508     499     250

  Fragments arranged by size: 

                 508     499     250
 
SfcI C'TryA_G

Cuts at:      0     127     286    1006    1257 
   Size:        127     159     720     251

  Fragments arranged by size: 

                 720     251     159     127
 
SmlI C'TyrA_G

Cuts at:      0     616    1257 
   Size:        616     641
 
StyI C'CwwG_G

Cuts at:      0     584    1257 
   Size:        584     673
 
TatI w'GTAC_w

Cuts at:      0     689    1257 
   Size:        689     568
 
Tth111I GACn'n_nGTC

Cuts at:      0    1203    1257 
   Size:       1203      54
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     591     747    1257 
   Size:        591     156     510

  Fragments arranged by size: 

                 591     510     156
 
ZraI GAC'GTC

Cuts at:      0     573     729    1257 
   Size:        573     156     528

  Fragments arranged by size: 

                 573     528     156
 

 Enzymes that do cut:

     AarI    AatII     AcuI     AfeI     AhdI    AlwNI     ApaI     AvaI
     BanI    BanII     BbsI     BglI Bme1580I    BmgBI     BpmI   Bpu10I
    BpuEI     BsaI    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI     BtgI    BtgZI     BtsI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV     FalI
    HaeII    Hin4I   HincII     MmeI     MscI     MslI   MspA1I     NaeI
     NcoI   NgoMIV     NotI     NspI    PflMI     PfoI    PpuMI   PspOMI
     PstI     SacI    SacII     SapI     SfcI     SmlI     StyI     TatI
  Tth111I     XcmI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI    AflII   AflIII     AgeI     AleI     AlfI
     AloI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII
     BlpI     BmrI     BmtI     BplI    BsaAI    BsaBI    BsaXI    BsiWI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     ClaI    CspCI     DraI   DraIII    EcoRI     FseI
     FspI    FspAI  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     NarI     NdeI     NheI     NruI     NsiI     PacI     PasI     PciI
     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI
    PvuII    RsrII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TstI     XbaI     XhoI     XmaI
     XmnI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com