Restriction map for pF1KE0733
##################################
# NO CUT ENZYMES (  136 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AviII       AvrII       
BamHI       BbeI        BbrPI       BbvCI       BciVI       
BclI        BfaI        BfrI        BglII       BlnI        
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       BsmI        
BspDI       BspEI       BspLU11I    BsrDI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstZ17I     Bsu36I      ClaI        CpoI        
DraI        DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FbaI        FseI        
FspI        HaeII       HgaI        Hin1I       HindIII     
HpaI        KasI        KpnI        KspI        MaeI        
MaeII       MamI        MfeI        MluI        MroI        
MseI        MunI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RsrII       SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SnaBI       SpeI        
SphI        Sse8387I    SspI        StuI        StyI        
SwaI        Tru9I       Tsp509I     XbaI        XcmI        
XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccII          30  CGCG                 CG^CG
ApaI          396  GGGCCC               GGGCC^C
AspHI         742  GAGCTC               G[AT]GC[AT]^C
AspI          226  GACTCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbsI          136  GTCTTC               GAAGAC(2/6)
BcgI          131  GCACCGTCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI          907  GCCCCTTTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          739  GCTGAGC              GC^T[ACGT]AGC
BpmI          166  CTCCAG               CTGGAG(16/14)
Bpu1102I      739  GCTGAGC              GC^T[ACGT]AGC
BpuAI         136  GTCTTC               GAAGAC(2/6)
BseRI         679  CTCCTC               GAGGAG(10/8)
BsgI          272  GTGCAG               GTGCAG(16/14)
BsiHKAI       742  GAGCTC               G[AT]GC[AT]^C
Bsp1407I      605  TGTACA               T^GTACA
BspHI         567  TCATGA               T^CATGA
BspMI         274  GCAGGT               ACCTGC(4/8)
BsrBI         349  GAGCGG               CCGCTC(-3/-3)
BsrFI         791  GCCGGT               [GA]^CCGG[CT]
BsrGI         605  TGTACA               T^GTACA
BstUI          30  CGCG                 CG^CG
BstXI         462  CCATTCCCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         739  GCTGAGC              GC^T[ACGT]AGC
CfoI           31  GCGC                 GCG^C
Cfr10I        791  GCCGGT               [GA]^CCGG[CT]
DraIII        401  CACTGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI          832  GAAGAG               CTCTTC(1/4)
HhaI           31  GCGC                 GCG^C
HinP1I         31  GCGC                 G^CGC
HincII          3  GTTGAC               GT[CT]^[GA]AC
HindII          3  GTTGAC               GT[CT]^[GA]AC
HphI          406  GGTGA                GGTGA(8/7)
Ksp632I       832  GAAGAG               CTCTTC(1/4)
MspA1I        420  CCGCTG               C[AC]G^C[GT]G
MvnI           30  CGCG                 CG^CG
NspI          235  ACATGC               [GA]CATG^[CT]
PaeR7I        346  CTCGAG               C^TCGAG
PflFI         226  GACTCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI          40  GGGACCC              [GA]G^G[AT]CC[CT]
RcaI          567  TCATGA               T^CATGA
SacI          742  GAGCTC               GAGCT^C
SfcI           16  CTACAG               C^T[GA][CT]AG
SgrAI         790  CGCCGGTG             C[GA]^CCGG[CT]G
SmaI           37  CCCGGG               CCC^GGG
SmlI          346  CTCGAG               C^T[CT][GA]AG
SspBI         605  TGTACA               T^GTACA
Tth111I       226  GACTCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI          346  CTCGAG               C^TCGAG
XmaCI          37  CCCGGG               C^CCGGG
XmaI           37  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AluI          738  AGCT                 AG^CT
              743  AGCT                
AvaI           37  CCCGGG               C^[CT]CG[GA]G
              346  CTCGAG              
BalI          173  TGGCCA               TGG^CCA
              432  TGGCCA              
BanII         396  GGGCCC               G[GA]GC[CT]^C
              742  GAGCTC              
BbvI          445  GCAGC                GCAGC(8/12)
              422  GCTGC               
BsmAI         319  GAGAC                GTCTC(1/5)
              763  GTCTC               
BsoBI          37  CCCGGG               C^[CT]CG[GA]G
              346  CTCGAG              
Cac8I         302  GCCTGC               GC[ACGT]^[ACGT]GC
              360  GCCCGC              
DdeI          228  CTCAG                C^T[ACGT]AG
              740  CTGAG               
DsaI          468  CCGTGG               C^C[GA][CT]GG
              522  CCGTGG              
EcoNI         111  CCTGGGTAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              710  CCTTTGAGAGG         
FauI           28  CCCGC                CCCGC(4/6)
              361  CCCGC               
MluNI         173  TGGCCA               TGG^CCA
              432  TGGCCA              
MscI          173  TGGCCA               TGG^CCA
              432  TGGCCA              
PflMI         435  CCAAGTAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              656  CCACTGTCTGG         
SfaNI         855  GATGC                GCATC(5/9)
              674  GCATC               
TseI          422  GCTGC                G^C[AT]GC
              445  GCAGC               
Van91I        435  CCAAGTAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              656  CCACTGTCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   54 enzymes) #
#########################################

AciI        AfaI        AlwI        AvaII       BanI        
BcnI        BmyI        BsaJI       BsiYI       BslI        
BsmFI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstYI       Cfr13I      DpnII       DraII       
EaeI        EcoO109I    EcoRII      Fnu4HI      FokI        
HaeIII      HapII       HinfI       HpaII       ItaI        
MaeIII      MboI        MboII       MflI        MnlI        
MslI        MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        RsaI        
Sau3AI      Sau96I      ScrFI       TaqI        TfiI        
Tsp45I      TspRI       TthHB8I     XhoII       
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