Restriction map for pF1KE0724
 (Linear) (Six-base) MAPSORT of: KIEE0724.seq  Check: 904  from: 1  to: 1245



KIEE0724.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:17  ..

AccI GT'mk_AC

Cuts at:      0     314    1245 
   Size:        314     931
 
Acc65I G'GTAC_C

Cuts at:      0     788    1245 
   Size:        788     457
 
AflIII A'CryG_T

Cuts at:      0     719    1245 
   Size:        719     526
 
AgeI A'CCGG_T

Cuts at:      0     269    1245 
   Size:        269     976
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     256     288     370     402    1245 
   Size:        256      32      82      32     843

  Fragments arranged by size: 

                 843     256      82      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1162    1245 
   Size:       1162      83
 
ApaI G_GGCC'C

Cuts at:      0     130     249     371    1245 
   Size:        130     119     122     874

  Fragments arranged by size: 

                 874     130     122     119
 
ApaLI G'TGCA_C

Cuts at:      0     466    1245 
   Size:        466     779
 
AvaI C'yCGr_G

Cuts at:      0      96     108     242     502     525     592    1245 
   Size:         96      12     134     260      23      67     653

  Fragments arranged by size: 

                 653     260     134      96      67      23      12
 
AvrII C'CTAG_G

Cuts at:      0     183    1245 
   Size:        183    1062
 
BanI G'GyrC_C

Cuts at:      0     788     884    1015    1245 
   Size:        788      96     131     230

  Fragments arranged by size: 

                 788     230     131      96
 
BanII G_rGCy'C

Cuts at:      0      97     130     179     249     371     764    1245 
   Size:         97      33      49      70     122     393     481

  Fragments arranged by size: 

                 481     393     122      97      70      49      33
 
BbeI G_GCGC'C

Cuts at:      0     888    1245 
   Size:        888     357
 
BbvCI CC'TCA_GC

Cuts at:      0    1046    1245 
   Size:       1046     199
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     135     169     942     976    1245 
   Size:        135      34     773      34     269

  Fragments arranged by size: 

                 773     269     135      34      34
 
BclI T'GATC_A

Cuts at:      0     446    1245 
   Size:        446     799
 
BglI GCCn_nnn'nGGC

Cuts at:      0     638    1245 
   Size:        638     607
 
BlpI GC'TnA_GC

Cuts at:      0     286    1245 
   Size:        286     959
 
Bme1580I G_kGCm'C

Cuts at:      0     130     249     371     470     643    1245 
   Size:        130     119     122      99     173     602

  Fragments arranged by size: 

                 602     173     130     122     119      99
 
BmgBI CAC'GTC

Cuts at:      0     495    1245 
   Size:        495     750
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     993    1245 
   Size:        993     252
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     283     376     397     724    1245 
   Size:        283      93      21     327     521

  Fragments arranged by size: 

                 521     327     283      93      21
 
Bpu10I CC'TnA_GC

Cuts at:      0    1046    1245 
   Size:       1046     199
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     400     748     763    1245 
   Size:        400     348      15     482

  Fragments arranged by size: 

                 482     400     348      15
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     589    1245 
   Size:        589     656
 
BsaAI yAC'GTr

Cuts at:      0     213     866    1245 
   Size:        213     653     379

  Fragments arranged by size: 

                 653     379     213
 
BsaBI GATnn'nnATC

Cuts at:      0     436    1245 
   Size:        436     809
 
BsaHI Gr'CG_yC

Cuts at:      0     302     885    1245 
   Size:        302     583     360

  Fragments arranged by size: 

                 583     360     302
 
BsaWI w'CCGG_w

Cuts at:      0      89     269     401    1245 
   Size:         89     180     132     844

  Fragments arranged by size: 

                 844     180     132      89
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     256     286     370     400    1021    1051    1245 
   Size:        256      30      84      30     621      30     194

  Fragments arranged by size: 

                 621     256     194      84      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      52     868    1227    1245 
   Size:         52     816     359      18

  Fragments arranged by size: 

                 816     359      52      18
 
BseYI C'CCAG_C

Cuts at:      0     363     370     711    1040    1064    1245 
   Size:        363       7     341     329      24     181

  Fragments arranged by size: 

                 363     341     329     181      24       7
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     241    1245 
   Size:        241    1004
 
BsiEI CG_ry'CG

Cuts at:      0     402     951    1133    1245 
   Size:        402     549     182     112

  Fragments arranged by size: 

                 549     402     182     112
 
BsiHKAI G_wGCw'C

Cuts at:      0      97     179     470     626     836    1245 
   Size:         97      82     291     156     210     409

  Fragments arranged by size: 

                 409     291     210     156      97      82
 
BsmI GAATG_Cn'

Cuts at:      0    1119    1245 
   Size:       1119     126
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      25    1245 
   Size:         25    1220
 
Bsp1286I G_dGCh'C

Cuts at:      0      97     130     179     249     371     470     626     643 
   Size:         97      33      49      70     122      99     156      17

Cuts at:    643     764     836    1245 
   Size:        121      72     409

  Fragments arranged by size: 

                 409     156     122     121      99      97      72      70
                  49      33      17
 
BspEI T'CCGG_A

Cuts at:      0      89    1245 
   Size:         89    1156
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     878     929    1040    1245 
   Size:        878      51     111     205

  Fragments arranged by size: 

                 878     205     111      51
 
BsrBI CCG'CTC

Cuts at:      0     786    1245 
   Size:        786     459
 
BsrFI r'CCGG_y

Cuts at:      0      61     269    1132    1245 
   Size:         61     208     863     113

  Fragments arranged by size: 

                 863     208     113      61
 
BssHII G'CGCG_C

Cuts at:      0     646    1245 
   Size:        646     599
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     354    1245 
   Size:        354     891
 
Bsu36I CC'TnA_GG

Cuts at:      0     308    1245 
   Size:        308     937
 
BtgI C'CryG_G

Cuts at:      0      81     347    1127    1245 
   Size:         81     266     780     118

  Fragments arranged by size: 

                 780     266     118      81
 
BtsI GCAGTG_nn'

Cuts at:      0     960    1245 
   Size:        960     285
 
EaeI y'GGCC_r

Cuts at:      0     572     860    1130    1134    1178    1245 
   Size:        572     288     270       4      44      67

  Fragments arranged by size: 

                 572     288     270      67      44       4
 
EagI C'GGCC_G

Cuts at:      0    1130    1245 
   Size:       1130     115
 
EarI CTCTTCn'nnn_

Cuts at:      0     937     949    1245 
   Size:        937      12     296

  Fragments arranged by size: 

                 937     296      12
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     169    1245 
   Size:        169    1076
 
EcoICRI GAG'CTC

Cuts at:      0      95     177    1245 
   Size:         95      82    1068

  Fragments arranged by size: 

                1068      95      82
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     283     376     397     724    1245 
   Size:        283      93      21     327     521

  Fragments arranged by size: 

                 521     327     283      93      21
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     676    1245 
   Size:        676     569
 
EcoO109I rG'GnC_Cy

Cuts at:      0     114     127     246     367     381    1245 
   Size:        114      13     119     121      14     864

  Fragments arranged by size: 

                 864     121     119     114      14      13
 
FseI GG_CCGG'CC

Cuts at:      0    1136    1245 
   Size:       1136     109
 
HaeII r_GCGC'y

Cuts at:      0     293     712     888    1162    1245 
   Size:        293     419     176     274      83

  Fragments arranged by size: 

                 419     293     274     176      83
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1021    1053    1245 
   Size:       1021      32     192

  Fragments arranged by size: 

                1021     192      32
 
KasI G'GCGC_C

Cuts at:      0     884    1245 
   Size:        884     361
 
KpnI G_GTAC'C

Cuts at:      0     792    1245 
   Size:        792     453
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1166    1245 
   Size:       1166      79
 
MscI TGG'CCA

Cuts at:      0     574     862    1245 
   Size:        574     288     383

  Fragments arranged by size: 

                 574     383     288
 
MslI CAynn'nnrTG

Cuts at:      0     454     724     829    1245 
   Size:        454     270     105     416

  Fragments arranged by size: 

                 454     416     270     105
 
MspA1I CmG'CkG

Cuts at:      0     255     444    1245 
   Size:        255     189     801

  Fragments arranged by size: 

                 801     255     189
 
NaeI GCC'GGC

Cuts at:      0      63    1134    1245 
   Size:         63    1071     111

  Fragments arranged by size: 

                1071     111      63
 
NarI GG'CG_CC

Cuts at:      0     885    1245 
   Size:        885     360
 
NcoI C'CATG_G

Cuts at:      0     347    1245 
   Size:        347     898
 
NgoMIV G'CCGG_C

Cuts at:      0      61    1132    1245 
   Size:         61    1071     113

  Fragments arranged by size: 

                1071     113      61
 
NspI r_CATG'y

Cuts at:      0     547     723    1245 
   Size:        547     176     522

  Fragments arranged by size: 

                 547     522     176
 
PciI A'CATG_T

Cuts at:      0     719    1245 
   Size:        719     526
 
PfoI T'CCnGG_A

Cuts at:      0    1052    1245 
   Size:       1052     193
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     256     288     370     402    1245 
   Size:        256      32      82      32     843

  Fragments arranged by size: 

                 843     256      82      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     114     381    1245 
   Size:        114     267     864

  Fragments arranged by size: 

                 864     267     114
 
PspOMI G'GGCC_C

Cuts at:      0     126     245     367    1245 
   Size:        126     119     122     878

  Fragments arranged by size: 

                 878     126     122     119
 
PstI C_TGCA'G

Cuts at:      0    1167    1235    1245 
   Size:       1167      68      10

  Fragments arranged by size: 

                1167      68      10
 
PvuII CAG'CTG

Cuts at:      0     255     444    1245 
   Size:        255     189     801

  Fragments arranged by size: 

                 801     255     189
 
RsrII CG'GwC_CG

Cuts at:      0     325    1245 
   Size:        325     920
 
SacI G_AGCT'C

Cuts at:      0      97     179    1245 
   Size:         97      82    1066

  Fragments arranged by size: 

                1066      97      82
 
SapI GCTCTTCn'nnn_

Cuts at:      0     937    1245 
   Size:        937     308
 
SfcI C'TryA_G

Cuts at:      0    1163    1231    1245 
   Size:       1163      68      14

  Fragments arranged by size: 

                1163      68      14
 
SfoI GGC'GCC

Cuts at:      0     886    1245 
   Size:        886     359
 
SmaI CCC'GGG

Cuts at:      0     244     527    1245 
   Size:        244     283     718

  Fragments arranged by size: 

                 718     283     244
 
SmlI C'TyrA_G

Cuts at:      0     415     763     778    1245 
   Size:        415     348      15     467

  Fragments arranged by size: 

                 467     415     348      15
 
SphI G_CATG'C

Cuts at:      0     547    1245 
   Size:        547     698
 
StyI C'CwwG_G

Cuts at:      0      56     183     347     575    1245 
   Size:         56     127     164     228     670

  Fragments arranged by size: 

                 670     228     164     127      56
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     313    1245 
   Size:        313     932
 
TatI w'GTAC_w

Cuts at:      0     338     581     728    1245 
   Size:        338     243     147     517

  Fragments arranged by size: 

                 517     338     243     147
 
XmaI C'CCGG_G

Cuts at:      0     242     525    1245 
   Size:        242     283     720

  Fragments arranged by size: 

                 720     283     242
 
XmnI GAAnn'nnTTC

Cuts at:      0     931    1245 
   Size:        931     314
 

 Enzymes that do cut:

     AccI   Acc65I   AflIII     AgeI     AloI    AlwNI     ApaI    ApaLI
     AvaI    AvrII     BanI    BanII     BbeI    BbvCI     BcgI     BclI
     BglI     BlpI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspMI
    BsrBI    BsrFI   BssHII    BstXI   Bsu36I     BtgI     BtsI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FseI
    HaeII    Hin4I     KasI     KpnI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NspI     PciI     PfoI     PpiI
    PpuMI   PspOMI     PstI    PvuII    RsrII     SacI     SapI     SfcI
     SfoI     SmaI     SmlI     SphI     StyI    TaqII     TatI     XmaI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AcuI     AfeI    AflII     AhdI     AleI
     AlfI     ApoI     AscI     AseI    AsiSI     BaeI    BamHI     BbsI
    BciVI    BfrBI    BglII     BmtI     BplI    BsiWI    BspHI    BsrDI
    BsrGI    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI    EcoRI    EcoRV     FalI
     FspI    FspAI   HincII  HindIII     HpaI     MfeI     MluI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI     PmeI
     PmlI    PshAI     PsiI    PspXI     PsrI     PvuI    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI    SnaBI
     SpeI     SrfI     SspI     StuI     SwaI     TstI  Tth111I     XbaI
     XcmI     XhoI     ZraI
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