Restriction map for pF1KE0707
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# NO CUT ENZYMES (  133 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        AlwNI       Aor51HI     ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AvrII       BamHI       BanI        BbeI        BbsI        
BciVI       BclI        BfaI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaBI       
BsaHI       BsaWI       BseAI       BsiWI       BsmBI       
BsmFI       BsmI        Bsp1407I    BspDI       BspEI       
BspLU11I    BsrBI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstYI       BstZ17I     CelII       Cfr10I      
ClaI        CpoI        DraI        DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MaeI        
MamI        MflI        MluI        MroI        MseI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PinAI       PmeI        PpuMI       PshAI       
PshBI       Psp1406I    RsrII       SacII       SalI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SspBI       
SspI        StuI        SwaI        TaqI        TfiI        
Tru9I       TthHB8I     XbaI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   41 enzymes) #
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AlwI         1270  GATCC                GGATC(4/5)
AocI          109  CCTGAGG              CC^T[ACGT]AGG
AspI          946  GACCGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         1117  CTCGGG               C^[CT]CG[GA]G
AviII         272  TGCGCA               TGC^GCA
BalI          731  TGGCCA               TGG^CCA
BbvCI        1164  CCTCAGC              CCTCAGC(-5/-2)
BcgI          858  GCAGCACGATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI          627  CCGGG                CC^[GC]GG
BglI          593  GCCTGCACGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseRI           5  CTCCTC               GAGGAG(10/8)
BsoBI        1117  CTCGGG               C^[CT]CG[GA]G
BspHI         737  TCATGA               T^CATGA
BsrI          634  CCAGT                ACTGG(1/-1)
BstXI         506  CCAGGTCCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        109  CCTGAGG              CC^T[ACGT]AGG
DraIII       1188  CACCCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco81I        109  CCTGAGG              CC^T[ACGT]AGG
FspI          272  TGCGCA               TGC^GCA
HaeII         220  GGCGCT               [GA]GCGC^[CT]
HphI          516  GGTGA                GGTGA(8/7)
MfeI           18  CAATTG               C^AATTG
MluNI         731  TGGCCA               TGG^CCA
MscI          731  TGGCCA               TGG^CCA
MunI           18  CAATTG               C^AATTG
NciI          627  CCGGG                CC^[GC]GG
NspI          656  GCATGC               [GA]CATG^[CT]
PflFI         946  GACCGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1224  CCACCCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuI          864  CGATCG               CGAT^CG
RcaI          737  TCATGA               T^CATGA
SacI          349  GAGCTC               GAGCT^C
SapI          711  GCTCTTC              GCTCTTC(1/4)
SbfI          845  CCTGCAGG             CCTGCA^GG
SmlI          145  CTCAAG               C^T[CT][GA]AG
SphI          656  GCATGC               GCATG^C
Sse8387I      845  CCTGCAGG             CCTGCA^GG
Tsp509I        19  AATT                 ^AATT
Tth111I       946  GACCGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1224  CCACCCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         1224  CCACCCCCTGGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


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# TWO CUT ENZYMES (   14 enzymes) #
###################################

AfaI           45  GTAC                 GT^AC
              150  GTAC                
Alw44I        490  GTGCAC               G^TGCAC
             1009  GTGCAC              
ApaLI         490  GTGCAC               G^TGCAC
             1009  GTGCAC              
BbrPI         616  CACGTG               CAC^GTG
             1006  CACGTG              
BsaAI         616  CACGTG               [CT]AC^GT[GA]
             1006  CACGTG              
BsiEI         864  CGATCG               CG[GA][CT]^CG
              945  CGACCG              
BsrDI          77  GCAATG               GCAATG(2/0)
              230  GCAATG              
EarI          295  CTCTTC               CTCTTC(1/4)
              712  CTCTTC              
Ksp632I       295  CTCTTC               CTCTTC(1/4)
              712  CTCTTC              
MslI          687  CATGGTAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              738  CATGAGCGTG          
PmaCI         616  CACGTG               CAC^GTG
             1006  CACGTG              
PmlI          616  CACGTG               CAC^GTG
             1006  CACGTG              
PstI          821  CTGCAG               CTGCA^G
              846  CTGCAG              
RsaI           45  GTAC                 GT^AC
              150  GTAC                


#########################################
# 3 OR MORE CUT ENZYMES (   70 enzymes) #
#########################################

AccII       AciI        AluI        ApaI        AspHI       
AvaII       BanII       BbvI        BmyI        BpmI        
BsaI        BsaJI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       Bsp1286I    BspMI       BssKI       
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HgaI        
HhaI        HinP1I      HinfI       HpaII       ItaI        
MaeII       MaeIII      MboI        MboII       MnlI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NcoI        NdeII       NlaIII      NlaIV       PleI        
PvuII       Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StyI        TseI        Tsp45I      TspRI       
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