Restriction map for pF1KE0703
##################################
# NO CUT ENZYMES (  176 enzymes) #
##################################

Acc65I      AccII       AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwI        AlwNI       AocI        Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AvaII       AviII       AvrII       BalI        BamHI       
BanI        BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BfaI        BfrI        BglI        
BlnI        BlpI        Bpu1102I    BsaBI       BsaI        
BsaWI       BseAI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsiYI       BslI        BsmBI       BsmFI       
BsmI        BsoBI       BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrFI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT14I     
FauI        FbaI        FseI        FspI        HaeII       
HgaI        HincII      HindII      HindIII     HpaI        
KasI        KpnI        Ksp632I     KspI        MaeI        
MamI        MfeI        MluI        MluNI       MroI        
MscI        MslI        MspA1I      MunI        MvnI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NlaIII      NotI        NruI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfaNI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SpeI        SphI        Sse8387I    
StuI        StyI        SwaI        Tth111I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AatII         606  GACGTC               GACGT^C
AccI          609  GTCTAC               GT^[AC][GT]AC
AciI           46  CCGC                 CCGC(-3/-1)
AcyI          606  GACGTC               G[GA]^CG[CT]C
ApaI          693  GGGCCC               GGGCC^C
BanII         693  GGGCCC               G[GA]GC[CT]^C
BbsI          675  GAAGAC               GAAGAC(2/6)
BcnI           18  CCCGG                CC^[GC]GG
BglII         179  AGATCT               A^GATCT
BmyI          693  GGGCCC               G[AGT]GC[ACT]^C
BpmI          131  CTGGAG               CTGGAG(16/14)
BpuAI         675  GAAGAC               GAAGAC(2/6)
BsaAI         629  TACGTA               [CT]AC^GT[GA]
BsaHI         606  GACGTC               G[GA]^CG[CT]C
BsaJI         690  CCAGGG               C^C[ACGT][ACGT]GG
BseRI         484  GAGGAG               GAGGAG(10/8)
BsmAI         826  GAGAC                GTCTC(1/5)
Bsp1286I      693  GGGCCC               G[AGT]GC[ACT]^C
Bsp1407I      709  TGTACA               T^GTACA
BspMI          81  GCAGGT               ACCTGC(4/8)
BsrGI         709  TGTACA               T^GTACA
BstYI         179  AGATCT               [GA]^GATC[CT]
Cac8I          89  GCAAGC               GC[ACGT]^[ACGT]GC
DpnII         180  GATC                 ^GATC
EcoT22I       263  ATGCAT               ATGCA^T
HapII          19  CCGG                 C^CGG
Hin1I         606  GACGTC               G[GA]^CG[CT]C
HpaII          19  CCGG                 C^CGG
HphI          334  GGTGA                GGTGA(8/7)
MaeIII        211  GTGAC                ^GT[ACGT]AC
MboI          180  GATC                 ^GATC
MflI          179  AGATCT               [GA]^GATC[CT]
MspI           19  CCGG                 C^CGG
MwoI           39  GCTTAATCCGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI           18  CCCGG                CC^[GC]GG
NdeII         180  GATC                 ^GATC
NsiI          263  ATGCAT               ATGCA^T
Sau3AI        180  GATC                 ^GATC
SmlI          740  CTCAAG               C^T[CT][GA]AG
SnaBI         629  TACGTA               TAC^GTA
SspBI         709  TGTACA               T^GTACA
SspI          403  AATATT               AAT^ATT
TaqI           59  TCGA                 T^CGA
TfiI          453  GAATC                G^A[AT]TC
Tsp45I        211  GTGAC                ^GT[GC]AC
TthHB8I        59  TCGA                 T^CGA
XhoII         179  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AluI          473  AGCT                 AG^CT
              528  AGCT                
BbvI          417  GCAGC                GCAGC(8/12)
              869  GCAGC               
BstF5I        288  GGATG                GGATG(2/0)
              479  GGATG               
DraI          391  TTTAAA               TTT^AAA
              493  TTTAAA              
DraII         692  AGGGCCC              [GA]G^G[ACGT]CC[CT]
              693  GGGCCCC             
EcoO109I      692  AGGGCCC              [GA]G^G[ACGT]CC[CT]
              693  GGGCCCC             
Fnu4HI        417  GCAGC                GC^[ACGT]GC
              869  GCAGC               
FokI          288  GGATG                GGATG(9/13)
              479  GGATG               
ItaI          417  GCAGC                GC^[ACGT]GC
              869  GCAGC               
MaeII         607  ACGT                 A^CGT
              630  ACGT                
NlaIV         693  GGGCCC               GG[ACGT]^[ACGT]CC
              694  GGCCCC              
TseI          417  GCAGC                G^C[AT]GC
              869  GCAGC               
Tsp509I       524  AATT                 ^AATT
              540  AATT                
TspRI         207  CACTG                CA[GC]TG[ACGT][ACGT]^
              434  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   21 enzymes) #
#########################################

AfaI        BsrI        BssKI       BstNI       CfoI        
Cfr13I      DdeI        EcoRII      HaeIII      HhaI        
HinP1I      HinfI       MboII       MnlI        MseI        
MvaI        PleI        RsaI        Sau96I      ScrFI       
Tru9I       
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