Restriction map for pF1KE0695
 (Linear) (Six-base) MAPSORT of: KIEE0695.seq  Check: 6615  from: 1  to: 852



KIEE0695.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:17  ..

Acc65I G'GTAC_C

Cuts at:      0      12     852 
   Size:         12     840
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     385     490     852 
   Size:        385     105     362

  Fragments arranged by size: 

                 385     362     105
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     814     846     852 
   Size:        814      32       6

  Fragments arranged by size: 

                 814      32       6
 
AlwNI CAG_nnn'CTG

Cuts at:      0     648     852 
   Size:        648     204
 
BanI G'GyrC_C

Cuts at:      0      12     286     447     852 
   Size:         12     274     161     405

  Fragments arranged by size: 

                 405     274     161      12
 
BanII G_rGCy'C

Cuts at:      0      70     228     852 
   Size:         70     158     624

  Fragments arranged by size: 

                 624     158      70
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     141     601     710     852 
   Size:        141     460     109     142

  Fragments arranged by size: 

                 460     142     141     109
 
BfrBI ATG'CAT

Cuts at:      0     637     852 
   Size:        637     215
 
BglI GCCn_nnn'nGGC

Cuts at:      0     232     852 
   Size:        232     620
 
BlpI GC'TnA_GC

Cuts at:      0      64     852 
   Size:         64     788
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     692     852 
   Size:        692     160
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      84     852 
   Size:         84     768
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     251     852 
   Size:        251     601
 
BsaWI w'CCGG_w

Cuts at:      0      70     242     852 
   Size:         70     172     610

  Fragments arranged by size: 

                 610     172      70
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     781     811     814     844     852 
   Size:        781      30       3      30       8

  Fragments arranged by size: 

                 781      30      30       8       3
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     556     802     835     852 
   Size:        556     246      33      17

  Fragments arranged by size: 

                 556     246      33      17
 
BsiEI CG_ry'CG

Cuts at:      0      83     243     852 
   Size:         83     160     609

  Fragments arranged by size: 

                 609     160      83
 
BsiHKAI G_wGCw'C

Cuts at:      0      70     852 
   Size:         70     782
 
BsmI GAATG_Cn'

Cuts at:      0     637     852 
   Size:        637     215
 
Bsp1286I G_dGCh'C

Cuts at:      0      70     228     852 
   Size:         70     158     624

  Fragments arranged by size: 

                 624     158      70
 
BspEI T'CCGG_A

Cuts at:      0      70     852 
   Size:         70     782
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     214     852 
   Size:        214     638
 
BtgI C'CryG_G

Cuts at:      0      77     852 
   Size:         77     775
 
BtsI GCAGTG_nn'

Cuts at:      0     170     852 
   Size:        170     682
 
EaeI y'GGCC_r

Cuts at:      0      80     780     852 
   Size:         80     700      72

  Fragments arranged by size: 

                 700      80      72
 
EagI C'GGCC_G

Cuts at:      0      80     852 
   Size:         80     772
 
EarI CTCTTCn'nnn_

Cuts at:      0     821     852 
   Size:        821      31
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19     852 
   Size:         19     833
 
EcoICRI GAG'CTC

Cuts at:      0      68     852 
   Size:         68     784
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     251     385     490     852 
   Size:        251     134     105     362

  Fragments arranged by size: 

                 362     251     134     105
 
HindIII A'AGCT_T

Cuts at:      0     397     607     852 
   Size:        397     210     245

  Fragments arranged by size: 

                 397     245     210
 
KpnI G_GTAC'C

Cuts at:      0      16     852 
   Size:         16     836
 
MscI TGG'CCA

Cuts at:      0     782     852 
   Size:        782      70
 
NsiI A_TGCA'T

Cuts at:      0     639     852 
   Size:        639     213
 
NspI r_CATG'y

Cuts at:      0     239     852 
   Size:        239     613
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     348     852 
   Size:        348     504
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     814     846     852 
   Size:        814      32       6

  Fragments arranged by size: 

                 814      32       6
 
PstI C_TGCA'G

Cuts at:      0     165     852 
   Size:        165     687
 
SacI G_AGCT'C

Cuts at:      0      70     852 
   Size:         70     782
 
SfcI C'TryA_G

Cuts at:      0     161     852 
   Size:        161     691
 
SphI G_CATG'C

Cuts at:      0     239     852 
   Size:        239     613
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     365     852 
   Size:        365     487
 
TatI w'GTAC_w

Cuts at:      0     689     852 
   Size:        689     163
 
XbaI T'CTAG_A

Cuts at:      0     663     852 
   Size:        663     189
 
XmnI GAAnn'nnTTC

Cuts at:      0     475     852 
   Size:        475     377
 

 Enzymes that do cut:

   Acc65I     AcuI     AloI    AlwNI     BanI    BanII     BbsI    BfrBI
     BglI     BlpI     BmrI     BplI     BpmI    BsaWI    BsaXI    BseRI
    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI   BstAPI     BtgI     BtsI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI  HindIII     KpnI
     MscI     NsiI     NspI    PflMI     PpiI     PstI     SacI     SfcI
     SphI    TaqII     TatI     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI
    BciVI     BclI    BglII Bme1580I    BmgBI     BmtI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BseYI     BsgI    BsiWI    BsmBI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII     HpaI
     KasI     MfeI     MluI     MmeI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI
     PciI     PfoI     PmeI     PmlI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SrfI     SspI     StuI     StyI
     SwaI     TstI  Tth111I     XcmI     XhoI     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com