Restriction map for
pF1KE0688
(Circular) (Six-base) MAPSORT of: pF1KE0688.seq Check: 3294 from: 1 to: 4316
pF1KE0688 4316 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AccI GT'mk_AC
Cuts at: 1354 1354
Size: 4316
Acc65I G'GTAC_C
Cuts at: 1332 1332
Size: 4316
AclI AA'CG_TT
Cuts at: 4156 4156
Size: 4316
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 298 364 2141 2573 3320 3588 181
Size: 117 66 1777 432 747 268 909
Fragments arranged by size:
1777 909 747 432 268 117 66
AflII C'TTAA_G
Cuts at: 1006 1006
Size: 4316
AflIII A'CryG_T
Cuts at: 1668 2772 3749 1668
Size: 1104 977 2235
Fragments arranged by size:
2235 1104 977
AgeI A'CCGG_T
Cuts at: 2704 2704
Size: 4316
AlwNI CAG_nnn'CTG
Cuts at: 571 867 888 1621 3188 571
Size: 296 21 733 1567 1699
Fragments arranged by size:
1699 1567 733 296 21
ApaLI G'TGCA_C
Cuts at: 3086 3086
Size: 4316
ApoI r'AATT_y
Cuts at: 1136 1180 1279 1320 3669 3925 1136
Size: 44 99 41 2349 256 1527
Fragments arranged by size:
2349 1527 256 99 44 41
AseI AT'TA_AT
Cuts at: 20 20
Size: 4316
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4316
AvaI C'yCGr_G
Cuts at: 674 1336 674
Size: 662 3654
AvrII C'CTAG_G
Cuts at: 126 126
Size: 4316
BamHI G'GATC_C
Cuts at: 348 1341 348
Size: 993 3323
BanI G'GyrC_C
Cuts at: 1144 1332 1746 1994 2029 1144
Size: 188 414 248 35 3431
Fragments arranged by size:
3431 414 248 188 35
BanII G_rGCy'C
Cuts at: 1330 2360 1330
Size: 1030 3286
BbeI G_GCGC'C
Cuts at: 1750 1998 1750
Size: 248 4068
BbsI GAAGACnn'nnnn_
Cuts at: 710 919 1073 710
Size: 209 154 3953
Fragments arranged by size:
3953 209 154
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 921 955 921
Size: 34 4282
BciVI GTATCCnnnnn_n'
Cuts at: 2214 2975 4316 2214
Size: 761 1341 2214
Fragments arranged by size:
2214 1341 761
BclI T'GATC_A
Cuts at: 1836 1836
Size: 4316
BfrBI ATG'CAT
Cuts at: 967 967
Size: 4316
BglII A'GATC_T
Cuts at: 769 1831 3868 769
Size: 1062 2037 1217
Fragments arranged by size:
2037 1217 1062
BlpI GC'TnA_GC
Cuts at: 1428 1428
Size: 4316
Bme1580I G_kGCm'C
Cuts at: 817 1149 1941 2034 3090 817
Size: 332 792 93 1056 2043
Fragments arranged by size:
2043 1056 792 332 93
BmrI ACTGGGnnnn_n'
Cuts at: 259 353 736 1528 1609 1944 4088 259
Size: 94 383 792 81 335 2144 487
Fragments arranged by size:
2144 792 487 383 335 94 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2683 2683
Size: 4316
Bpu10I CC'TnA_GC
Cuts at: 393 889 393
Size: 496 3820
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 684 1492 2371 2863 3161 684
Size: 808 879 492 298 1839
Fragments arranged by size:
1839 879 808 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 130 549 4020 130
Size: 419 3471 426
Fragments arranged by size:
3471 426 419
BsaAI yAC'GTr
Cuts at: 1669 1669
Size: 4316
BsaBI GATnn'nnATC
Cuts at: 6 986 1830 3867 6
Size: 980 844 2037 455
Fragments arranged by size:
2037 980 844 455
BsaHI Gr'CG_yC
Cuts at: 950 1747 1995 2697 4193 950
Size: 797 248 702 1496 1073
Fragments arranged by size:
1496 1073 797 702 248
BsaWI w'CCGG_w
Cuts at: 778 1505 2026 2704 2978 3125 3536 778
Size: 727 521 678 274 147 411 1558
Fragments arranged by size:
1558 727 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 282 615 282
Size: 333 3983
BseYI C'CCAG_C
Cuts at: 337 1741 2499 3076 3572 337
Size: 1404 758 577 496 1081
Fragments arranged by size:
1404 1081 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1858 1904 3112 104
Size: 1754 46 1208 1308
Fragments arranged by size:
1754 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 895 1330 2108 2298 3090 895
Size: 435 778 190 792 2121
Fragments arranged by size:
2121 792 778 435 190
Bsp1286I G_dGCh'C
Cuts at: 817 895 1149 1330 1941 2034 2108 2298 2360
Size: 78 254 181 611 93 74 190 62
Cuts at: 2360 3090 817
Size: 730 2043
Fragments arranged by size:
2043 730 611 254 190 181 93 78
74 62
BspEI T'CCGG_A
Cuts at: 3536 3536
Size: 4316
BspHI T'CATG_A
Cuts at: 3492 3492
Size: 4316
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1366 1580 1882 2263 1366
Size: 214 302 381 3419
Fragments arranged by size:
3419 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2608 2662 4146 4311 50
Size: 2558 54 1484 165 55
Fragments arranged by size:
2558 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 629 2228 629
Size: 1599 2717
BsrFI r'CCGG_y
Cuts at: 302 2314 2495 2704 302
Size: 2012 181 209 1914
Fragments arranged by size:
2012 1914 209 181
BssSI C'ACGA_G
Cuts at: 2587 2945 2587
Size: 358 3958
BstAPI GCAn_nnn'nTGC
Cuts at: 1693 1693
Size: 4316
BstBI TT'CG_AA
Cuts at: 2677 2677
Size: 4316
BstXI CCAn_nnnn'nTGG
Cuts at: 648 648
Size: 4316
BstYI r'GATC_y
Cuts at: 1 348 561 769 1341 1808 1831 2166 2412
Size: 347 213 208 572 467 23 335 246
Cuts at: 2412 3424 3510 3522 3868 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 572 467 449 347 346 335 246
213 208 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2266 2447 2610 2266
Size: 181 163 3972
Fragments arranged by size:
3972 181 163
BtsI GCAGTG_nn'
Cuts at: 893 1559 893
Size: 666 3650
DraI TTT'AAA
Cuts at: 1315 1315
Size: 4316
DrdI GACnn_nn'nnGTC
Cuts at: 2022 2880 2022
Size: 858 3458
EaeI y'GGCC_r
Cuts at: 1901 2075 2466 2493 3838 1901
Size: 174 391 27 1345 2379
Fragments arranged by size:
2379 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1901 1901
Size: 4316
EarI CTCTTCn'nnn_
Cuts at: 686 2339 2549 686
Size: 1653 210 2453
Fragments arranged by size:
2453 1653 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 635 2524 2834 2980 4126 635
Size: 1889 310 146 1146 825
Fragments arranged by size:
1889 1146 825 310 146
EcoICRI GAG'CTC
Cuts at: 1328 1328
Size: 4316
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 298 364 2141 2573 3320 3588 181
Size: 117 66 1777 432 747 268 909
Fragments arranged by size:
1777 909 747 432 268 117 66
EcoO109I rG'GnC_Cy
Cuts at: 885 1455 885
Size: 570 3746
EcoRI G'AATT_C
Cuts at: 1320 1320
Size: 4316
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 864 2335 864
Size: 1471 2845
FspI TGC'GCA
Cuts at: 2097 2097
Size: 4316
HaeII r_GCGC'y
Cuts at: 450 1750 1998 3020 3999 450
Size: 1300 248 1022 979 767
Fragments arranged by size:
1300 1022 979 767 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 546 578 2158 2188 2190 2220 546
Size: 32 1580 30 2 30 2642
Fragments arranged by size:
2642 1580 32 30 30 2
HincII GTy'rAC
Cuts at: 1218 1355 1218
Size: 137 4179
KasI G'GCGC_C
Cuts at: 1746 1994 1746
Size: 248 4068
KpnI G_GTAC'C
Cuts at: 1336 1336
Size: 4316
MluI A'CGCG_T
Cuts at: 3749 3749
Size: 4316
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 799 1095 2987 3171 799
Size: 296 1892 184 1944
Fragments arranged by size:
1944 1892 296 184
MscI TGG'CCA
Cuts at: 2077 2077
Size: 4316
MslI CAynn'nnrTG
Cuts at: 2305 2432 2305
Size: 127 4189
MspA1I CmG'CkG
Cuts at: 337 474 993 1427 1741 2101 3114 3359 337
Size: 137 519 434 314 360 1013 245 1294
Fragments arranged by size:
1294 1013 519 434 360 314 245 137
NaeI GCC'GGC
Cuts at: 2497 2497
Size: 4316
NarI GG'CG_CC
Cuts at: 1747 1995 1747
Size: 248 4068
NgoMIV G'CCGG_C
Cuts at: 2495 2495
Size: 4316
NsiI A_TGCA'T
Cuts at: 969 969
Size: 4316
NspI r_CATG'y
Cuts at: 331 1262 1369 1869 2776 331
Size: 931 107 500 907 1871
Fragments arranged by size:
1871 931 907 500 107
PciI A'CATG_T
Cuts at: 2772 2772
Size: 4316
PflMI CCAn_nnn'nTGG
Cuts at: 867 867
Size: 4316
PfoI T'CCnGG_A
Cuts at: 851 851
Size: 4316
PmeI GTTT'AAAC
Cuts at: 1315 1315
Size: 4316
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3455 3487 3455
Size: 32 4284
PstI C_TGCA'G
Cuts at: 188 375 1363 2048 188
Size: 187 988 685 2456
Fragments arranged by size:
2456 988 685 187
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4316
PvuII CAG'CTG
Cuts at: 337 1741 2101 337
Size: 1404 360 2552
Fragments arranged by size:
2552 1404 360
RsrII CG'GwC_CG
Cuts at: 2511 2511
Size: 4316
SacI G_AGCT'C
Cuts at: 1330 1330
Size: 4316
SalI G'TCGA_C
Cuts at: 1353 1353
Size: 4316
SapI GCTCTTCn'nnn_
Cuts at: 2339 2549 2339
Size: 210 4106
SbfI CC_TGCA'GG
Cuts at: 1363 1363
Size: 4316
ScaI AGT'ACT
Cuts at: 3833 3833
Size: 4316
SfcI C'TryA_G
Cuts at: 33 184 371 1359 2044 3037 3228 33
Size: 151 187 988 685 993 191 1121
Fragments arranged by size:
1121 993 988 685 191 187 151
SfoI GGC'GCC
Cuts at: 1748 1996 1748
Size: 248 4068
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4316
SmaI CCC'GGG
Cuts at: 1338 1338
Size: 4316
SmlI C'TyrA_G
Cuts at: 699 1006 1471 2386 2878 3140 699
Size: 307 465 915 492 262 1875
Fragments arranged by size:
1875 915 492 465 307 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4316
SphI G_CATG'C
Cuts at: 1369 1869 1369
Size: 500 3816
StyI C'CwwG_G
Cuts at: 126 1085 1450 1804 126
Size: 959 365 354 2638
Fragments arranged by size:
2638 959 365 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 654 780 1899 2032 2491 2700 654
Size: 126 1119 133 459 209 2270
Fragments arranged by size:
2270 1119 459 209 133 126
TatI w'GTAC_w
Cuts at: 250 3831 250
Size: 3581 735
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1109 1141 3833 3865 1109
Size: 32 2692 32 1560
Fragments arranged by size:
2692 1560 32 32
Tth111I GACn'n_nGTC
Cuts at: 2113 2113
Size: 4316
XbaI T'CTAG_A
Cuts at: 1347 1347
Size: 4316
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1811 1811
Size: 4316
XmaI C'CCGG_G
Cuts at: 1336 1336
Size: 4316
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NsiI NspI
PciI PflMI PfoI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI BaeI BbvCI BglI BmgBI BmtI BpmI BsaXI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI PacI PasI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI