Restriction map for pF1KE0688
 (Linear) (Six-base) MAPSORT of: KIEE0688.seq  Check: 6736  from: 1  to: 1203



KIEE0688.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:17  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     190     256    1203 
   Size:         73     117      66     947

  Fragments arranged by size: 

                 947     117      73      66
 
AflII C'TTAA_G

Cuts at:      0     898    1203 
   Size:        898     305
 
AlwNI CAG_nnn'CTG

Cuts at:      0     463     759     780    1203 
   Size:        463     296      21     423

  Fragments arranged by size: 

                 463     423     296      21
 
ApoI r'AATT_y

Cuts at:      0    1028    1072    1171    1203 
   Size:       1028      44      99      32

  Fragments arranged by size: 

                1028      99      44      32
 
AvaI C'yCGr_G

Cuts at:      0     566    1203 
   Size:        566     637
 
AvrII C'CTAG_G

Cuts at:      0      18    1203 
   Size:         18    1185
 
BamHI G'GATC_C

Cuts at:      0     240    1203 
   Size:        240     963
 
BanI G'GyrC_C

Cuts at:      0    1036    1203 
   Size:       1036     167
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     602     811     965    1203 
   Size:        602     209     154     238

  Fragments arranged by size: 

                 602     238     209     154
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     813     847    1203 
   Size:        813      34     356

  Fragments arranged by size: 

                 813     356      34
 
BfrBI ATG'CAT

Cuts at:      0     859    1203 
   Size:        859     344
 
BglII A'GATC_T

Cuts at:      0     661    1203 
   Size:        661     542
 
Bme1580I G_kGCm'C

Cuts at:      0     709    1041    1203 
   Size:        709     332     162

  Fragments arranged by size: 

                 709     332     162
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     151     245     628    1203 
   Size:        151      94     383     575

  Fragments arranged by size: 

                 575     383     151      94
 
Bpu10I CC'TnA_GC

Cuts at:      0     285     781    1203 
   Size:        285     496     422

  Fragments arranged by size: 

                 496     422     285
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     576    1203 
   Size:        576     627
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      22     441    1203 
   Size:         22     419     762

  Fragments arranged by size: 

                 762     419      22
 
BsaBI GATnn'nnATC

Cuts at:      0     878    1203 
   Size:        878     325
 
BsaHI Gr'CG_yC

Cuts at:      0     842    1203 
   Size:        842     361
 
BsaWI w'CCGG_w

Cuts at:      0     670    1203 
   Size:        670     533
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     174     507    1203 
   Size:        174     333     696

  Fragments arranged by size: 

                 696     333     174
 
BseYI C'CCAG_C

Cuts at:      0     229    1203 
   Size:        229     974
 
BsiHKAI G_wGCw'C

Cuts at:      0     787    1203 
   Size:        787     416
 
Bsp1286I G_dGCh'C

Cuts at:      0     709     787    1041    1203 
   Size:        709      78     254     162

  Fragments arranged by size: 

                 709     254     162      78
 
BsrDI GCAATG_nn'

Cuts at:      0     521    1203 
   Size:        521     682
 
BsrFI r'CCGG_y

Cuts at:      0     194    1203 
   Size:        194    1009
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     540    1203 
   Size:        540     663
 
BstYI r'GATC_y

Cuts at:      0     240     453     661    1203 
   Size:        240     213     208     542

  Fragments arranged by size: 

                 542     240     213     208
 
BtsI GCAGTG_nn'

Cuts at:      0     785    1203 
   Size:        785     418
 
EarI CTCTTCn'nnn_

Cuts at:      0     578    1203 
   Size:        578     625
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     527    1203 
   Size:        527     676
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     190     256    1203 
   Size:         73     117      66     947

  Fragments arranged by size: 

                 947     117      73      66
 
EcoO109I rG'GnC_Cy

Cuts at:      0     777    1203 
   Size:        777     426
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     756    1203 
   Size:        756     447
 
HaeII r_GCGC'y

Cuts at:      0     342    1203 
   Size:        342     861
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     438     470    1203 
   Size:        438      32     733

  Fragments arranged by size: 

                 733     438      32
 
HincII GTy'rAC

Cuts at:      0    1110    1203 
   Size:       1110      93
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     691     987    1203 
   Size:        691     296     216

  Fragments arranged by size: 

                 691     296     216
 
MspA1I CmG'CkG

Cuts at:      0     229     366     885    1203 
   Size:        229     137     519     318

  Fragments arranged by size: 

                 519     318     229     137
 
NsiI A_TGCA'T

Cuts at:      0     861    1203 
   Size:        861     342
 
NspI r_CATG'y

Cuts at:      0     223    1154    1203 
   Size:        223     931      49

  Fragments arranged by size: 

                 931     223      49
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     759    1203 
   Size:        759     444
 
PfoI T'CCnGG_A

Cuts at:      0     743    1203 
   Size:        743     460
 
PstI C_TGCA'G

Cuts at:      0      80     267    1203 
   Size:         80     187     936

  Fragments arranged by size: 

                 936     187      80
 
PvuII CAG'CTG

Cuts at:      0     229    1203 
   Size:        229     974
 
SfcI C'TryA_G

Cuts at:      0      76     263    1203 
   Size:         76     187     940

  Fragments arranged by size: 

                 940     187      76
 
SmlI C'TyrA_G

Cuts at:      0     591     898    1203 
   Size:        591     307     305

  Fragments arranged by size: 

                 591     307     305
 
StyI C'CwwG_G

Cuts at:      0      18     977    1203 
   Size:         18     959     226

  Fragments arranged by size: 

                 959     226      18
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     546     672    1203 
   Size:        546     126     531

  Fragments arranged by size: 

                 546     531     126
 
TatI w'GTAC_w

Cuts at:      0     142    1203 
   Size:        142    1061
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1001    1033    1203 
   Size:       1001      32     170

  Fragments arranged by size: 

                1001     170      32
 

 Enzymes that do cut:

     AcuI    AflII    AlwNI     ApoI     AvaI    AvrII    BamHI     BanI
     BbsI     BcgI    BfrBI    BglII Bme1580I     BmrI   Bpu10I    BpuEI
     BsaI    BsaBI    BsaHI    BsaWI    BseRI    BseYI  BsiHKAI Bsp1286I
    BsrDI    BsrFI    BstXI    BstYI     BtsI     EarI     EciI  Eco57MI
 EcoO109I     FalI    HaeII    Hin4I   HincII     MmeI   MspA1I     NsiI
     NspI    PflMI     PfoI     PstI    PvuII     SfcI     SmlI     StyI
    TaqII     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     BaeI    BanII     BbeI    BbvCI    BciVI     BclI     BglI
     BlpI    BmgBI     BmtI     BplI     BpmI    BsaAI    BsaXI     BsgI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I
     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     PacI     PasI     PciI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI
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