(Circular) (Six-base) MAPSORT of: pF1KE0680.seq Check: 6063 from: 1 to: 4310 pF1KE0680 4310 bp With 182 enzymes: SgfI * April 24, 2009 13:17 .. AccI GT'mk_AC Cuts at: 1348 1348 Size: 4310 Acc65I G'GTAC_C Cuts at: 1326 1326 Size: 4310 AclI AA'CG_TT Cuts at: 4150 4150 Size: 4310 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 928 2135 2567 3314 3582 928 Size: 1207 432 747 268 1656 Fragments arranged by size: 1656 1207 747 432 268 AfeI AGC'GCT Cuts at: 742 742 Size: 4310 AflIII A'CryG_T Cuts at: 656 1662 2766 3743 656 Size: 1006 1104 977 1223 Fragments arranged by size: 1223 1104 1006 977 AgeI A'CCGG_T Cuts at: 2698 2698 Size: 4310 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1067 1099 1067 Size: 32 4278 AlwNI CAG_nnn'CTG Cuts at: 192 213 1615 3182 192 Size: 21 1402 1567 1320 Fragments arranged by size: 1567 1402 1320 21 ApaLI G'TGCA_C Cuts at: 3080 3080 Size: 4310 ApoI r'AATT_y Cuts at: 1314 3663 3919 1314 Size: 2349 256 1705 Fragments arranged by size: 2349 1705 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4310 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4310 AvaI C'yCGr_G Cuts at: 385 1004 1330 385 Size: 619 326 3365 Fragments arranged by size: 3365 619 326 BamHI G'GATC_C Cuts at: 344 1335 344 Size: 991 3319 BanI G'GyrC_C Cuts at: 1326 1740 1988 2023 1326 Size: 414 248 35 3613 Fragments arranged by size: 3613 414 248 35 BanII G_rGCy'C Cuts at: 227 833 1004 1324 2354 227 Size: 606 171 320 1030 2183 Fragments arranged by size: 2183 1030 606 320 171 BbeI G_GCGC'C Cuts at: 1744 1992 1744 Size: 248 4062 BbsI GAAGACnn'nnnn_ Cuts at: 934 934 Size: 4310 BciVI GTATCCnnnnn_n' Cuts at: 2208 2969 4310 2208 Size: 761 1341 2208 Fragments arranged by size: 2208 1341 761 BclI T'GATC_A Cuts at: 1830 1830 Size: 4310 BglII A'GATC_T Cuts at: 1825 3862 1825 Size: 2037 2273 BlpI GC'TnA_GC Cuts at: 1422 1422 Size: 4310 Bme1580I G_kGCm'C Cuts at: 1935 2028 3084 1935 Size: 93 1056 3161 Fragments arranged by size: 3161 1056 93 BmgBI CAC'GTC Cuts at: 932 932 Size: 4310 BmrI ACTGGGnnnn_n' Cuts at: 1522 1603 1938 4082 1522 Size: 81 335 2144 1750 Fragments arranged by size: 2144 1750 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2677 2677 Size: 4310 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 184 277 757 1042 184 Size: 93 480 285 3452 Fragments arranged by size: 3452 480 285 93 Bpu10I CC'TnA_GC Cuts at: 799 799 Size: 4310 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1486 2365 2857 3155 1486 Size: 879 492 298 2641 Fragments arranged by size: 2641 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 4014 4014 Size: 4310 BsaAI yAC'GTr Cuts at: 659 1663 659 Size: 1004 3306 BsaBI GATnn'nnATC Cuts at: 6 1824 3861 6 Size: 1818 2037 455 Fragments arranged by size: 2037 1818 455 BsaHI Gr'CG_yC Cuts at: 119 1741 1989 2691 4187 119 Size: 1622 248 702 1496 242 Fragments arranged by size: 1622 1496 702 248 242 BsaWI w'CCGG_w Cuts at: 144 293 590 1166 1499 2020 2698 2972 3119 Size: 149 297 576 333 521 678 274 147 Cuts at: 3119 3530 144 Size: 411 924 Fragments arranged by size: 924 678 576 521 411 333 297 274 149 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 163 235 403 805 1204 163 Size: 72 168 402 399 3269 Fragments arranged by size: 3269 402 399 168 72 BseYI C'CCAG_C Cuts at: 611 912 1735 2493 3070 3566 611 Size: 301 823 758 577 496 1355 Fragments arranged by size: 1355 823 758 577 496 301 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 550 550 Size: 4310 BsiEI CG_ry'CG Cuts at: 104 1137 1852 1898 3106 104 Size: 1033 715 46 1208 1308 Fragments arranged by size: 1308 1208 1033 715 46 BsiHKAI G_wGCw'C Cuts at: 227 485 1324 2102 2292 3084 227 Size: 258 839 778 190 792 1453 Fragments arranged by size: 1453 839 792 778 258 190 Bsp1286I G_dGCh'C Cuts at: 227 485 833 1004 1324 1935 2028 2102 2292 Size: 258 348 171 320 611 93 74 190 Cuts at: 2292 2354 3084 227 Size: 62 730 1453 Fragments arranged by size: 1453 730 611 348 320 258 190 171 93 74 62 BspEI T'CCGG_A Cuts at: 144 3530 144 Size: 3386 924 BspHI T'CATG_A Cuts at: 3486 3486 Size: 4310 BspMI ACCTGCnnnn'nnnn_ Cuts at: 791 1360 1574 1876 2257 791 Size: 569 214 302 381 2844 Fragments arranged by size: 2844 569 381 302 214 BsrBI CCG'CTC Cuts at: 50 153 2602 2656 4140 4305 50 Size: 103 2449 54 1484 165 55 Fragments arranged by size: 2449 1484 165 103 55 54 BsrDI GCAATG_nn' Cuts at: 311 2222 311 Size: 1911 2399 BsrFI r'CCGG_y Cuts at: 2308 2489 2698 2308 Size: 181 209 3920 Fragments arranged by size: 3920 209 181 BsrGI T'GTAC_A Cuts at: 407 407 Size: 4310 BssSI C'ACGA_G Cuts at: 2581 2939 2581 Size: 358 3952 BstAPI GCAn_nnn'nTGC Cuts at: 192 1687 192 Size: 1495 2815 BstBI TT'CG_AA Cuts at: 2671 2671 Size: 4310 BstYI r'GATC_y Cuts at: 1 344 498 1169 1335 1802 1825 2160 2406 Size: 343 154 671 166 467 23 335 246 Cuts at: 2406 3418 3504 3516 3862 1 Size: 1012 86 12 346 449 Fragments arranged by size: 1012 671 467 449 346 343 335 246 166 154 86 23 12 Bsu36I CC'TnA_GG Cuts at: 538 788 845 538 Size: 250 57 4003 Fragments arranged by size: 4003 250 57 BtgI C'CryG_G Cuts at: 107 989 107 Size: 882 3428 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2260 2441 2604 2260 Size: 181 163 3966 Fragments arranged by size: 3966 181 163 BtsI GCAGTG_nn' Cuts at: 485 1553 485 Size: 1068 3242 DraI TTT'AAA Cuts at: 1309 1309 Size: 4310 DraIII CAC_nnn'GTG Cuts at: 1161 1161 Size: 4310 DrdI GACnn_nn'nnGTC Cuts at: 2016 2874 2016 Size: 858 3452 EaeI y'GGCC_r Cuts at: 749 1134 1895 2069 2460 2487 3832 749 Size: 385 761 174 391 27 1345 1227 Fragments arranged by size: 1345 1227 761 391 385 174 27 EagI C'GGCC_G Cuts at: 1134 1895 1134 Size: 761 3549 EarI CTCTTCn'nnn_ Cuts at: 2333 2543 2333 Size: 210 4100 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 195 1144 2518 2828 2974 4120 195 Size: 949 1374 310 146 1146 385 Fragments arranged by size: 1374 1146 949 385 310 146 EcoICRI GAG'CTC Cuts at: 225 1322 225 Size: 1097 3213 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 184 277 757 928 1042 2135 2567 3314 3582 Size: 93 480 171 114 1093 432 747 268 Cuts at: 3582 184 Size: 912 Fragments arranged by size: 1093 912 747 480 432 268 171 114 93 EcoNI CCTnn'n_nnAGG Cuts at: 217 409 787 217 Size: 192 378 3740 Fragments arranged by size: 3740 378 192 EcoO109I rG'GnC_Cy Cuts at: 534 780 1449 534 Size: 246 669 3395 Fragments arranged by size: 3395 669 246 EcoRI G'AATT_C Cuts at: 1314 1314 Size: 4310 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2329 2329 Size: 4310 FspI TGC'GCA Cuts at: 2091 2091 Size: 4310 HaeII r_GCGC'y Cuts at: 213 744 945 1744 1992 3014 3993 213 Size: 531 201 799 248 1022 979 530 Fragments arranged by size: 1022 979 799 531 530 248 201 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 998 1030 2152 2182 2184 2214 998 Size: 32 1122 30 2 30 3094 Fragments arranged by size: 3094 1122 32 30 30 2 HincII GTy'rAC Cuts at: 1349 1349 Size: 4310 KasI G'GCGC_C Cuts at: 1740 1988 1740 Size: 248 4062 KpnI G_GTAC'C Cuts at: 1330 1330 Size: 4310 MluI A'CGCG_T Cuts at: 3743 3743 Size: 4310 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2981 3165 2981 Size: 184 4126 MscI TGG'CCA Cuts at: 751 2071 751 Size: 1320 2990 MslI CAynn'nnrTG Cuts at: 2299 2426 2299 Size: 127 4183 MspA1I CmG'CkG Cuts at: 1421 1735 2095 3108 3353 1421 Size: 314 360 1013 245 2378 Fragments arranged by size: 2378 1013 360 314 245 NaeI GCC'GGC Cuts at: 2491 2491 Size: 4310 NarI GG'CG_CC Cuts at: 1741 1989 1741 Size: 248 4062 NcoI C'CATG_G Cuts at: 107 107 Size: 4310 NgoMIV G'CCGG_C Cuts at: 2489 2489 Size: 4310 NspI r_CATG'y Cuts at: 1363 1863 2770 1363 Size: 500 907 2903 Fragments arranged by size: 2903 907 500 PasI CC'CwG_GG Cuts at: 634 634 Size: 4310 PciI A'CATG_T Cuts at: 2766 2766 Size: 4310 PflMI CCAn_nnn'nTGG Cuts at: 354 354 Size: 4310 PfoI T'CCnGG_A Cuts at: 719 719 Size: 4310 PmeI GTTT'AAAC Cuts at: 1309 1309 Size: 4310 PmlI CAC'GTG Cuts at: 659 659 Size: 4310 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3449 3481 3449 Size: 32 4278 PpuMI rG'GwC_Cy Cuts at: 780 780 Size: 4310 PstI C_TGCA'G Cuts at: 377 1357 2042 377 Size: 980 685 2645 Fragments arranged by size: 2645 980 685 PvuI CG_AT'CG Cuts at: 104 104 Size: 4310 PvuII CAG'CTG Cuts at: 1735 2095 1735 Size: 360 3950 RsrII CG'GwC_CG Cuts at: 888 2505 888 Size: 1617 2693 SacI G_AGCT'C Cuts at: 227 1324 227 Size: 1097 3213 SalI G'TCGA_C Cuts at: 1347 1347 Size: 4310 SapI GCTCTTCn'nnn_ Cuts at: 2333 2543 2333 Size: 210 4100 SbfI CC_TGCA'GG Cuts at: 1357 1357 Size: 4310 ScaI AGT'ACT Cuts at: 3827 3827 Size: 4310 SfcI C'TryA_G Cuts at: 33 373 1353 2038 3031 3222 33 Size: 340 980 685 993 191 1121 Fragments arranged by size: 1121 993 980 685 340 191 SfoI GGC'GCC Cuts at: 1742 1990 1742 Size: 248 4062 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4310 SmaI CCC'GGG Cuts at: 1332 1332 Size: 4310 SmlI C'TyrA_G Cuts at: 1465 2380 2872 3134 1465 Size: 915 492 262 2641 Fragments arranged by size: 2641 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4310 SphI G_CATG'C Cuts at: 1363 1863 1363 Size: 500 3810 StuI AGG'CCT Cuts at: 604 604 Size: 4310 StyI C'CwwG_G Cuts at: 107 1444 1798 107 Size: 1337 354 2619 Fragments arranged by size: 2619 1337 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1893 2026 2485 2694 1893 Size: 133 459 209 3509 Fragments arranged by size: 3509 459 209 133 TatI w'GTAC_w Cuts at: 407 983 3825 407 Size: 576 2842 892 Fragments arranged by size: 2842 892 576 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3827 3859 3827 Size: 32 4278 Tth111I GACn'n_nGTC Cuts at: 118 2107 118 Size: 1989 2321 XbaI T'CTAG_A Cuts at: 1341 1341 Size: 4310 XcmI CCAnnnn_n'nnnnTGG Cuts at: 243 1805 243 Size: 1562 2748 XmaI C'CCGG_G Cuts at: 1330 1330 Size: 4310 XmnI GAAnn'nnTTC Cuts at: 319 319 Size: 4310 Enzymes that do cut: AccI Acc65I AclI AcuI AfeI AflIII AgeI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AflII AhdI AleI AlfI ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmtI BsaXI BsiWI BsmI BsmBI BssHII BstEII BstXI BstZ17I ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI