################################## # NO CUT ENZYMES ( 178 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BbvI BcgI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsaI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT14I EcoT22I FauI FbaI Fnu4HI FseI FspI HaeII Hin1I HpaI ItaI KasI KpnI Ksp632I KspI MaeI MamI MfeI MluI MluNI MroI MscI MspA1I MunI MvnI MwoI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TseI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AccI 1073 GTAGAC GT^[AC][GT]AC AfaI 171 GTAC GT^AC AlwI 980 GATCC GGATC(4/5) BciVI 1056 GTATCC GTATCC(6/5) BmyI 394 GGGCAC G[AGT]GC[ACT]^C BsaAI 1053 CACGTA [CT]AC^GT[GA] BsaWI 505 ACCGGA [AT]^CCGG[AT] BseRI 52 GAGGAG GAGGAG(10/8) Bsp1286I 394 GGGCAC G[AGT]GC[ACT]^C BsrDI 783 GCAATG GCAATG(2/0) BstBI 566 TTCGAA TT^CGAA BstYI 979 AGATCC [GA]^GATC[CT] CfoI 1168 GCGC GCG^C DpnII 980 GATC ^GATC EcoRV 467 GATATC GAT^ATC HaeIII 18 GGCC GG^CC HapII 506 CCGG C^CGG HgaI 572 GACGC GACGC(5/10) HhaI 1168 GCGC GCG^C HinP1I 1168 GCGC G^CGC HincII 443 GTCAAC GT[CT]^[GA]AC HindII 443 GTCAAC GT[CT]^[GA]AC HindIII 299 AAGCTT A^AGCTT HpaII 506 CCGG C^CGG MaeII 1054 ACGT A^CGT MboI 980 GATC ^GATC MflI 979 AGATCC [GA]^GATC[CT] MspI 506 CCGG C^CGG NdeI 854 CATATG CA^TATG NdeII 980 GATC ^GATC NspV 566 TTCGAA TT^CGAA RsaI 171 GTAC GT^AC Sau3AI 980 GATC ^GATC SfaNI 102 GATGC GCATC(5/9) SfuI 566 TTCGAA TT^CGAA TfiI 498 GAATC G^A[AT]TC Tsp509I 225 AATT ^AATT XhoII 979 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AluI 300 AGCT AG^CT 627 AGCT Asp700I 456 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 792 GAAAGCATTC BbsI 232 GAAGAC GAAGAC(2/6) 569 GAAGAC BpuAI 232 GAAGAC GAAGAC(2/6) 569 GAAGAC BsmAI 200 GTCTC GTCTC(1/5) 638 GTCTC BstF5I 27 GGATG GGATG(2/0) 594 GGATG DraII 8 AGGACCC [GA]G^G[ACGT]CC[CT] 1084 AGGACCC EcoO109I 8 AGGACCC [GA]G^G[ACGT]CC[CT] 1084 AGGACCC FokI 27 GGATG GGATG(9/13) 594 GGATG PleI 198 GAGTC GAGTC(4/5) 1002 GAGTC PpuMI 8 AGGACCC [GA]G^G[AT]CC[CT] 1084 AGGACCC SfcI 90 CTACAG C^T[GA][CT]AG 131 CTATAG TaqI 567 TCGA T^CGA 735 TCGA Tsp45I 386 GTGAC ^GT[GC]AC 1004 GTCAC TthHB8I 567 TCGA T^CGA 735 TCGA XmnI 456 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 792 GAAAGCATTC ######################################### # 3 OR MORE CUT ENZYMES ( 26 enzymes) # ######################################### AvaII BpmI BsaJI BsiYI BslI BsmI BsrI BssKI BstNI Cfr13I DdeI EcoRII HinfI HphI MaeIII MboII MnlI MseI MslI MvaI NlaIII NlaIV Sau96I ScrFI Tru9I TspRI