Restriction map for
pF1KE0671
(Circular) (Six-base) MAPSORT of: pF1KE0671.seq Check: 6850 from: 1 to: 3887
pF1KE0671 3887 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 401 401
Size: 3887
AccI GT'mk_AC
Cuts at: 925 925
Size: 3887
Acc65I G'GTAC_C
Cuts at: 253 903 253
Size: 650 3237
AclI AA'CG_TT
Cuts at: 3727 3727
Size: 3887
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1712 2144 2891 3159 1712
Size: 432 747 268 2440
Fragments arranged by size:
2440 747 432 268
AflIII A'CryG_T
Cuts at: 1239 2343 3320 1239
Size: 1104 977 1806
Fragments arranged by size:
1806 1104 977
AgeI A'CCGG_T
Cuts at: 2275 2275
Size: 3887
AlwNI CAG_nnn'CTG
Cuts at: 718 1192 2759 718
Size: 474 1567 1846
Fragments arranged by size:
1846 1567 474
ApaI G_GGCC'C
Cuts at: 180 180
Size: 3887
ApaLI G'TGCA_C
Cuts at: 2657 2657
Size: 3887
ApoI r'AATT_y
Cuts at: 891 3240 3496 891
Size: 2349 256 1282
Fragments arranged by size:
2349 1282 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3887
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3887
AvaI C'yCGr_G
Cuts at: 235 907 235
Size: 672 3215
BamHI G'GATC_C
Cuts at: 912 912
Size: 3887
BanI G'GyrC_C
Cuts at: 253 903 1317 1565 1600 253
Size: 650 414 248 35 2540
Fragments arranged by size:
2540 650 414 248 35
BanII G_rGCy'C
Cuts at: 180 901 1931 180
Size: 721 1030 2136
Fragments arranged by size:
2136 1030 721
BbeI G_GCGC'C
Cuts at: 1321 1569 1321
Size: 248 3639
BciVI GTATCCnnnnn_n'
Cuts at: 1785 2546 3887 1785
Size: 761 1341 1785
Fragments arranged by size:
1785 1341 761
BclI T'GATC_A
Cuts at: 1407 1407
Size: 3887
BglI GCCn_nnn'nGGC
Cuts at: 379 379
Size: 3887
BglII A'GATC_T
Cuts at: 1402 3439 1402
Size: 2037 1850
BlpI GC'TnA_GC
Cuts at: 999 999
Size: 3887
Bme1580I G_kGCm'C
Cuts at: 180 768 1512 1605 2661 180
Size: 588 744 93 1056 1406
Fragments arranged by size:
1406 1056 744 588 93
BmgBI CAC'GTC
Cuts at: 819 819
Size: 3887
BmrI ACTGGGnnnn_n'
Cuts at: 167 1099 1180 1515 3659 167
Size: 932 81 335 2144 395
Fragments arranged by size:
2144 932 395 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2254 2254
Size: 3887
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 498 498
Size: 3887
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1063 1942 2434 2732 1063
Size: 879 492 298 2218
Fragments arranged by size:
2218 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3591 3591
Size: 3887
BsaAI yAC'GTr
Cuts at: 427 1240 427
Size: 813 3074
BsaBI GATnn'nnATC
Cuts at: 6 1401 3438 6
Size: 1395 2037 455
Fragments arranged by size:
2037 1395 455
BsaHI Gr'CG_yC
Cuts at: 1318 1566 2268 3764 1318
Size: 248 702 1496 1441
Fragments arranged by size:
1496 1441 702 248
BsaWI w'CCGG_w
Cuts at: 567 1076 1597 2275 2549 2696 3107 567
Size: 509 521 678 274 147 411 1347
Fragments arranged by size:
1347 678 521 509 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 253 313 695 253
Size: 60 382 3445
Fragments arranged by size:
3445 382 60
BseYI C'CCAG_C
Cuts at: 223 258 442 690 1312 2070 2647 3143 223
Size: 35 184 248 622 758 577 496 967
Fragments arranged by size:
967 758 622 577 496 248 184 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 591 607 760 591
Size: 16 153 3718
Fragments arranged by size:
3718 153 16
BsiEI CG_ry'CG
Cuts at: 104 1429 1475 2683 104
Size: 1325 46 1208 1308
Fragments arranged by size:
1325 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 901 1679 1869 2661 901
Size: 778 190 792 2127
Fragments arranged by size:
2127 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 180 768 901 1512 1605 1679 1869 1931 2661
Size: 588 133 611 93 74 190 62 730
Cuts at: 2661 180
Size: 1406
Fragments arranged by size:
1406 730 611 588 190 133 93 74
62
BspEI T'CCGG_A
Cuts at: 567 3107 567
Size: 2540 1347
BspHI T'CATG_A
Cuts at: 3063 3063
Size: 3887
BspMI ACCTGCnnnn'nnnn_
Cuts at: 401 937 1151 1453 1834 401
Size: 536 214 302 381 2454
Fragments arranged by size:
2454 536 381 302 214
BsrBI CCG'CTC
Cuts at: 50 153 2179 2233 3717 3882 50
Size: 103 2026 54 1484 165 55
Fragments arranged by size:
2026 1484 165 103 55 54
BsrDI GCAATG_nn'
Cuts at: 1799 1799
Size: 3887
BsrFI r'CCGG_y
Cuts at: 1885 2066 2275 1885
Size: 181 209 3497
Fragments arranged by size:
3497 209 181
BsrGI T'GTAC_A
Cuts at: 723 723
Size: 3887
BssSI C'ACGA_G
Cuts at: 476 2158 2516 476
Size: 1682 358 1847
Fragments arranged by size:
1847 1682 358
BstAPI GCAn_nnn'nTGC
Cuts at: 216 402 700 1264 216
Size: 186 298 564 2839
Fragments arranged by size:
2839 564 298 186
BstBI TT'CG_AA
Cuts at: 2248 2248
Size: 3887
BstYI r'GATC_y
Cuts at: 1 303 912 1379 1402 1737 1983 2995 3081
Size: 302 609 467 23 335 246 1012 86
Cuts at: 3081 3093 3439 1
Size: 12 346 449
Fragments arranged by size:
1012 609 467 449 346 335 302 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 668 668
Size: 3887
BtgI C'CryG_G
Cuts at: 107 107
Size: 3887
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1837 2018 2181 1837
Size: 181 163 3543
Fragments arranged by size:
3543 181 163
BtsI GCAGTG_nn'
Cuts at: 608 694 716 768 832 1130 608
Size: 86 22 52 64 298 3365
Fragments arranged by size:
3365 298 86 64 52 22
DraI TTT'AAA
Cuts at: 886 886
Size: 3887
DraIII CAC_nnn'GTG
Cuts at: 597 597
Size: 3887
DrdI GACnn_nn'nnGTC
Cuts at: 1593 2451 1593
Size: 858 3029
EaeI y'GGCC_r
Cuts at: 371 812 848 1472 1646 2037 2064 3409 371
Size: 441 36 624 174 391 27 1345 849
Fragments arranged by size:
1345 849 624 441 391 174 36 27
EagI C'GGCC_G
Cuts at: 1472 1472
Size: 3887
EarI CTCTTCn'nnn_
Cuts at: 1910 2120 1910
Size: 210 3677
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2095 2405 2551 3697 2095
Size: 310 146 1146 2285
Fragments arranged by size:
2285 1146 310 146
EcoICRI GAG'CTC
Cuts at: 899 899
Size: 3887
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 498 1712 2144 2891 3159 498
Size: 1214 432 747 268 1226
Fragments arranged by size:
1226 1214 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 238 238
Size: 3887
EcoO109I rG'GnC_Cy
Cuts at: 127 177 227 1026 127
Size: 50 50 799 2988
Fragments arranged by size:
2988 799 50 50
EcoRI G'AATT_C
Cuts at: 891 891
Size: 3887
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1906 1906
Size: 3887
FspI TGC'GCA
Cuts at: 1668 1668
Size: 3887
HaeII r_GCGC'y
Cuts at: 1321 1569 2591 3570 1321
Size: 248 1022 979 1638
Fragments arranged by size:
1638 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1729 1759 1761 1791 1729
Size: 30 2 30 3825
Fragments arranged by size:
3825 30 30 2
HincII GTy'rAC
Cuts at: 926 926
Size: 3887
KasI G'GCGC_C
Cuts at: 1317 1565 1317
Size: 248 3639
KpnI G_GTAC'C
Cuts at: 257 907 257
Size: 650 3237
MluI A'CGCG_T
Cuts at: 3320 3320
Size: 3887
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 557 885 2558 2742 557
Size: 328 1673 184 1702
Fragments arranged by size:
1702 1673 328 184
MscI TGG'CCA
Cuts at: 1648 1648
Size: 3887
MslI CAynn'nnrTG
Cuts at: 1876 2003 1876
Size: 127 3760
MspA1I CmG'CkG
Cuts at: 165 998 1312 1672 2685 2930 165
Size: 833 314 360 1013 245 1122
Fragments arranged by size:
1122 1013 833 360 314 245
NaeI GCC'GGC
Cuts at: 2068 2068
Size: 3887
NarI GG'CG_CC
Cuts at: 1318 1566 1318
Size: 248 3639
NcoI C'CATG_G
Cuts at: 107 107
Size: 3887
NgoMIV G'CCGG_C
Cuts at: 2066 2066
Size: 3887
NspI r_CATG'y
Cuts at: 940 1440 2347 940
Size: 500 907 2480
Fragments arranged by size:
2480 907 500
PciI A'CATG_T
Cuts at: 2343 2343
Size: 3887
PmeI GTTT'AAAC
Cuts at: 886 886
Size: 3887
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3026 3058 3026
Size: 32 3855
PpuMI rG'GwC_Cy
Cuts at: 127 127
Size: 3887
PspOMI G'GGCC_C
Cuts at: 176 176
Size: 3887
PspXI vC'TCGA_Gb
Cuts at: 235 235
Size: 3887
PstI C_TGCA'G
Cuts at: 398 934 1619 398
Size: 536 685 2666
Fragments arranged by size:
2666 685 536
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3887
PvuII CAG'CTG
Cuts at: 1312 1672 1312
Size: 360 3527
RsrII CG'GwC_CG
Cuts at: 2082 2082
Size: 3887
SacI G_AGCT'C
Cuts at: 901 901
Size: 3887
SalI G'TCGA_C
Cuts at: 924 924
Size: 3887
SapI GCTCTTCn'nnn_
Cuts at: 1910 2120 1910
Size: 210 3677
SbfI CC_TGCA'GG
Cuts at: 934 934
Size: 3887
ScaI AGT'ACT
Cuts at: 3404 3404
Size: 3887
SfcI C'TryA_G
Cuts at: 33 394 930 1615 2608 2799 33
Size: 361 536 685 993 191 1121
Fragments arranged by size:
1121 993 685 536 361 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 379 379
Size: 3887
SfoI GGC'GCC
Cuts at: 1319 1567 1319
Size: 248 3639
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3887
SmaI CCC'GGG
Cuts at: 909 909
Size: 3887
SmlI C'TyrA_G
Cuts at: 235 1042 1957 2449 2711 235
Size: 807 915 492 262 1411
Fragments arranged by size:
1411 915 807 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3887
SphI G_CATG'C
Cuts at: 940 1440 940
Size: 500 3387
StyI C'CwwG_G
Cuts at: 107 1021 1375 107
Size: 914 354 2619
Fragments arranged by size:
2619 914 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1470 1603 2062 2271 1470
Size: 133 459 209 3086
Fragments arranged by size:
3086 459 209 133
TatI w'GTAC_w
Cuts at: 723 3402 723
Size: 2679 1208
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 295 327 3404 3436 295
Size: 32 3077 32 746
Fragments arranged by size:
3077 746 32 32
Tth111I GACn'n_nGTC
Cuts at: 131 1684 131
Size: 1553 2334
XbaI T'CTAG_A
Cuts at: 918 918
Size: 3887
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 522 1382 522
Size: 860 3027
XhoI C'TCGA_G
Cuts at: 235 235
Size: 3887
XmaI C'CCGG_G
Cuts at: 907 907
Size: 3887
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PmeI PpiI PpuMI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmtI Bpu10I
BsaXI BsiWI BsmI BsmBI BssHII BstEII BstXI BstZ17I
ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PshAI PsiI PsrI SacII SanDI SexAI
SgrAI SnaBI SrfI SspI StuI SwaI XmnI ZraI