Restriction map for pF1KE0661
##################################
# NO CUT ENZYMES (  154 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AflII       AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaII       
AvrII       BanII       BbeI        BbrPI       BclI        
BfaI        BfrI        BlnI        BlpI        BmyI        
Bpu1102I    BsaAI       BsaI        BsaWI       BseAI       
BsiHKAI     BsiWI       BsmAI       BsmBI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DraI        DraIII      EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FauI        FbaI        FseI        HaeII       HincII      
HindII      HindIII     HinfI       HpaI        KasI        
KpnI        Ksp632I     KspI        MaeI        MfeI        
MluI        MroI        MseI        MslI        MunI        
MvnI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        SfaNI       SfiI        SfoI        
SfuI        SgrAI       SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TfiI        Tru9I       Tsp509I     Tth111I     
Van91I      XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AcyI           70  GACGCC               G[GA]^CG[CT]C
AflIII        405  ACACGT               A^C[GA][CT]GT
AvaI          152  CCCGGG               C^[CT]CG[GA]G
AviII          80  TGCGCA               TGC^GCA
BalI          666  TGGCCA               TGG^CCA
BamHI         210  GGATCC               G^GATCC
BanI          612  GGCACC               G^G[CT][GA]CC
BbsI           97  GTCTTC               GAAGAC(2/6)
BbvCI         679  CCTCAGC              CCTCAGC(-5/-2)
BcgI           83  GCAAGTACTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         347  GGATAC               GTATCC(6/5)
BglI          193  GCCGAAAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII          35  AGATCT               A^GATCT
BpuAI          97  GTCTTC               GAAGAC(2/6)
BsaBI         211  GATCCCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI          70  GACGCC               G[GA]^CG[CT]C
BseRI         112  GAGGAG               GAGGAG(10/8)
BsgI          382  GTGCAG               GTGCAG(16/14)
BsiEI         132  CGACCG               CG[GA][CT]^CG
BsiYI         135  CCGGCAGACGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          135  CCGGCAGACGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI          701  GAATGC               GAATGC(1/-1)
BsoBI         152  CCCGGG               C^[CT]CG[GA]G
BspMI         351  ACCTGC               ACCTGC(4/8)
BsrFI         134  ACCGGC               [GA]^CCGG[CT]
BstF5I        319  CATCC                GGATG(2/0)
CfoI           81  GCGC                 GCG^C
Cfr10I        134  ACCGGC               [GA]^CCGG[CT]
Cfr13I        200  GGGCC                G^G[ACGT]CC
DraII         199  AGGGCCT              [GA]G^G[ACGT]CC[CT]
DrdI          141  GACGGGCAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          173  CCATGG               C^C[GA][CT]GG
EaeI          666  TGGCCA               [CT]^GGCC[GA]
EcoO109I      199  AGGGCCT              [GA]G^G[ACGT]CC[CT]
FokI          319  CATCC                GGATG(9/13)
FspI           80  TGCGCA               TGC^GCA
HgaI           70  GACGC                GACGC(5/10)
HhaI           81  GCGC                 GCG^C
Hin1I          70  GACGCC               G[GA]^CG[CT]C
HinP1I         81  GCGC                 G^CGC
MamI          211  GATCCCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         666  TGGCCA               TGG^CCA
MscI          666  TGGCCA               TGG^CCA
NcoI          173  CCATGG               C^CATGG
Sau96I        200  GGGCC                G^G[ACGT]CC
ScaI           86  AGTACT               AGT^ACT
SexAI         288  ACCAGGT              A^CC[AT]GGT
SmaI          152  CCCGGG               CCC^GGG
Tsp45I        169  GTCAC                ^GT[GC]AC
TspRI         456  CACTG                CA[GC]TG[ACGT][ACGT]^
XcmI          242  CCAACGTGAACCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         152  CCCGGG               C^CCGGG
XmaI          152  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

BpmI          542  CTCCAG               CTGGAG(16/14)
              458  CTGGAG              
BsmFI         619  GGGAC                GGGAC(10/14)
              150  GTCCC               
BstYI          35  AGATCT               [GA]^GATC[CT]
              210  GGATCC              
EcoT14I       173  CCATGG               C^C[AT][AT]GG
              274  CCAAGG              
MaeIII        169  GTCAC                ^GT[ACGT]AC
              562  GTTAC               
MboII          38  TCTTC                GAAGA(8/7)
               98  TCTTC               
MflI           35  AGATCT               [GA]^GATC[CT]
              210  GGATCC              
NlaIII        174  CATG                 CATG^
              282  CATG                
NlaIV         210  GGATCC               GG[ACGT]^[ACGT]CC
              612  GGCACC              
StyI          173  CCATGG               C^C[AT][AT]GG
              274  CCAAGG              
TaqI           92  TCGA                 T^CGA
              110  TCGA                
TthHB8I        92  TCGA                 T^CGA
              110  TCGA                
XhoII          35  AGATCT               [GA]^GATC[CT]
              210  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   38 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
BbvI        BcnI        BsaJI       BsrI        BssKI       
BstAPI      BstNI       Cac8I       DdeI        DpnII       
EcoRII      Fnu4HI      HaeIII      HapII       HpaII       
HphI        ItaI        MaeII       MboI        MnlI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       PstI        PvuII       RsaI        Sau3AI      
ScrFI       SfcI        TseI        
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