Restriction map for pF1KE0656
 (Linear) (Six-base) MAPSORT of: KIEE0656.seq  Check: 4247  from: 1  to: 1164



KIEE0656.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:16  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     944    1164 
   Size:        944     220
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     409     703     799     836    1164 
   Size:        409     294      96      37     328

  Fragments arranged by size: 

                 409     328     294      96      37
 
AflIII A'CryG_T

Cuts at:      0      32      34     854    1164 
   Size:         32       2     820     310

  Fragments arranged by size: 

                 820     310      32       2
 
AleI CACnn'nnGTG

Cuts at:      0     365    1164 
   Size:        365     799
 
ApoI r'AATT_y

Cuts at:      0      25     120     802     976    1164 
   Size:         25      95     682     174     188

  Fragments arranged by size: 

                 682     188     174      95      25
 
AvrII C'CTAG_G

Cuts at:      0     333    1164 
   Size:        333     831
 
BclI T'GATC_A

Cuts at:      0     264    1164 
   Size:        264     900
 
BfrBI ATG'CAT

Cuts at:      0     654    1164 
   Size:        654     510
 
BglII A'GATC_T

Cuts at:      0     142    1164 
   Size:        142    1022
 
BlpI GC'TnA_GC

Cuts at:      0     719    1164 
   Size:        719     445
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1029    1164 
   Size:       1029     135
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      76    1086    1164 
   Size:         76    1010      78

  Fragments arranged by size: 

                1010      78      76
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1051    1164 
   Size:       1051     113
 
BsaAI yAC'GTr

Cuts at:      0      35    1164 
   Size:         35    1129
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     157     187    1164 
   Size:        157      30     977

  Fragments arranged by size: 

                 977     157      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     239     353     385     451    1009    1164 
   Size:        239     114      32      66     558     155

  Fragments arranged by size: 

                 558     239     155     114      66      32
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      99    1164 
   Size:         99    1065
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     367     922    1164 
   Size:        367     555     242

  Fragments arranged by size: 

                 555     367     242
 
BspHI T'CATG_A

Cuts at:      0     461     620    1164 
   Size:        461     159     544

  Fragments arranged by size: 

                 544     461     159
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     944    1164 
   Size:        944     220
 
BsrBI CCG'CTC

Cuts at:      0     295    1164 
   Size:        295     869
 
BsrGI T'GTAC_A

Cuts at:      0     662    1164 
   Size:        662     502
 
BstYI r'GATC_y

Cuts at:      0     142    1164 
   Size:        142    1022
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     858    1164 
   Size:        858     306
 
ClaI AT'CG_AT

Cuts at:      0     578    1164 
   Size:        578     586
 
EaeI y'GGCC_r

Cuts at:      0     351     377     749    1164 
   Size:        351      26     372     415

  Fragments arranged by size: 

                 415     372     351      26
 
EarI CTCTTCn'nnn_

Cuts at:      0       7      80    1030    1164 
   Size:          7      73     950     134

  Fragments arranged by size: 

                 950     134      73       7
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      76     409     703     799     836    1086    1164 
   Size:         76     333     294      96      37     250      78

  Fragments arranged by size: 

                 333     294     250      96      78      76      37
 
EcoRI G'AATT_C

Cuts at:      0      25    1164 
   Size:         25    1139
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     804    1164 
   Size:        804     360
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     127     157     159     189    1164 
   Size:        127      30       2      30     975

  Fragments arranged by size: 

                 975     127      30      30       2
 
HincII GTy'rAC

Cuts at:      0     237     300    1164 
   Size:        237      63     864

  Fragments arranged by size: 

                 864     237      63
 
HindIII A'AGCT_T

Cuts at:      0    1018    1164 
   Size:       1018     146
 
MscI TGG'CCA

Cuts at:      0     751    1164 
   Size:        751     413
 
MslI CAynn'nnrTG

Cuts at:      0     365     853    1164 
   Size:        365     488     311

  Fragments arranged by size: 

                 488     365     311
 
MspA1I CmG'CkG

Cuts at:      0      54     471     495    1164 
   Size:         54     417      24     669

  Fragments arranged by size: 

                 669     417      54      24
 
NsiI A_TGCA'T

Cuts at:      0     656    1164 
   Size:        656     508
 
NspI r_CATG'y

Cuts at:      0     654     858    1164 
   Size:        654     204     306

  Fragments arranged by size: 

                 654     306     204
 
PciI A'CATG_T

Cuts at:      0     854    1164 
   Size:        854     310
 
PmlI CAC'GTG

Cuts at:      0      35    1164 
   Size:         35    1129
 
PsiI TTA'TAA

Cuts at:      0      41    1164 
   Size:         41    1123
 
PstI C_TGCA'G

Cuts at:      0     955    1164 
   Size:        955     209
 
PvuII CAG'CTG

Cuts at:      0      54     471     495    1164 
   Size:         54     417      24     669

  Fragments arranged by size: 

                 669     417      54      24
 
SbfI CC_TGCA'GG

Cuts at:      0     955    1164 
   Size:        955     209
 
SfcI C'TryA_G

Cuts at:      0     951    1164 
   Size:        951     213
 
SmlI C'TyrA_G

Cuts at:      0    1066    1164 
   Size:       1066      98
 
StyI C'CwwG_G

Cuts at:      0     333    1001    1164 
   Size:        333     668     163

  Fragments arranged by size: 

                 668     333     163
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     901    1164 
   Size:        901     263
 
TatI w'GTAC_w

Cuts at:      0     662    1164 
   Size:        662     502
 
Tth111I GACn'n_nGTC

Cuts at:      0     526    1164 
   Size:        526     638
 
XmnI GAAnn'nnTTC

Cuts at:      0     977    1164 
   Size:        977     187
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AleI     ApoI    AvrII     BclI    BfrBI
    BglII     BlpI     BplI     BpmI    BpuEI    BsaAI    BsaXI    BseRI
     BsgI    BsmBI    BspHI    BspMI    BsrBI    BsrGI    BstYI    BtgZI
     ClaI     EaeI     EarI  Eco57MI    EcoRI     FalI    Hin4I   HincII
  HindIII     MscI     MslI   MspA1I     NsiI     NspI     PciI     PmlI
     PsiI     PstI    PvuII     SbfI     SfcI     SmlI     StyI    TaqII
     TatI  Tth111I     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI     BaeI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BglI Bme1580I    BmgBI     BmrI     BmtI   Bpu10I
     BsaI    BsaBI    BsaHI    BsaWI    BseYI    BsiEI  BsiHKAI    BsiWI
     BsmI Bsp1286I    BspEI    BsrDI    BsrFI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI     BtsI    CspCI
     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRV     FseI     FspI    FspAI    HaeII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     PacI     PasI    PflMI     PfoI     PmeI
     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI     TstI     XbaI     XcmI     XhoI     XmaI
     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com