Restriction map for pF1KE0654
##################################
# NO CUT ENZYMES (  158 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AviII       AvrII       
BamHI       BanI        BanII       BbeI        BbrPI       
BbvCI       BcgI        BclI        BcnI        BfrI        
BglII       BlnI        BlpI        BmyI        BpmI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BseRI       BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmI        Bsp1286I    Bsp1407I    
BspDI       BspEI       BspLU11I    BspMI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       BstBI       
BstUI       BstYI       Bsu36I      Cac8I       CelII       
CfoI        Cfr10I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoRI       EcoT22I     FbaI        
FseI        FspI        HaeII       HapII       HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        HpaII       KasI        KpnI        Ksp632I     
KspI        MamI        MflI        MluI        MroI        
MspI        MvnI        NaeI        NarI        NciI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        XbaI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccI          646  GTATAC               GT^[AC][GT]AC
AcsI          725  AAATTC               [GA]^AATT[CT]
AlwI          136  GATCC                GGATC(4/5)
ApoI          725  AAATTC               [GA]^AATT[CT]
AspI         1038  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          404  CCCGAG               C^[CT]CG[GA]G
BciVI         120  GGATAC               GTATCC(6/5)
BsaJI        1054  CCATGG               C^C[ACGT][ACGT]GG
BsmAI         454  GTCTC                GTCTC(1/5)
BsmFI        1062  GGGAC                GGGAC(10/14)
BsoBI         404  CCCGAG               C^[CT]CG[GA]G
BspHI         159  TCATGA               T^CATGA
BsrBI         560  CCGCTC               CCGCTC(-3/-3)
BssKI        1008  CCAGG                ^CC[ACGT]GG
Bst1107I      646  GTATAC               GTA^TAC
BstEII         34  GGTAACC              G^GT[ACGT]ACC
BstNI        1008  CCAGG                CC^[AT]GG
BstPI          34  GGTAACC              G^GT[ACGT]ACC
BstZ17I       646  GTATAC               GTA^TAC
DsaI         1054  CCATGG               C^C[GA][CT]GG
EcoO65I        34  GGTAACC              G^GT[ACGT]ACC
EcoRII       1008  CCAGG                ^CC[AT]GG
EcoRV         902  GATATC               GAT^ATC
EcoT14I      1054  CCATGG               C^C[AT][AT]GG
FauI          559  CCCGC                CCCGC(4/6)
HgaI          690  GCGTC                GACGC(5/10)
MaeII          29  ACGT                 A^CGT
MfeI          794  CAATTG               C^AATTG
MunI          794  CAATTG               C^AATTG
MvaI         1008  CCAGG                CC^[AT]GG
NcoI         1054  CCATGG               C^CATGG
PflFI        1038  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1066  CCAAATTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI          179  CTGCAG               CTGCA^G
RcaI          159  TCATGA               T^CATGA
ScrFI        1008  CCAGG                CC^[ACGT]GG
SfcI          179  CTGCAG               C^T[GA][CT]AG
SfiI          919  GGCCAGTGGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI         1088  CTCAAG               C^T[CT][GA]AG
StyI         1054  CCATGG               C^C[AT][AT]GG
TfiI          891  GAATC                G^A[AT]TC
Tsp45I         52  GTGAC                ^GT[GC]AC
Tth111I      1038  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1066  CCAAATTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AciI          560  CCGC                 CCGC(-3/-1)
             1084  CCGC                
AvaII          91  GGACC                G^G[AT]CC
             1063  GGACC               
BbsI          521  GAAGAC               GAAGAC(2/6)
             1044  GTCTTC              
BfaI          251  CTAG                 C^TAG
              626  CTAG                
BglI          920  GCCAGTGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1086  GCCTCAAGGGC         
BpuAI         521  GAAGAC               GAAGAC(2/6)
             1044  GTCTTC              
BsgI         1049  CTGCAC               GTGCAG(16/14)
              782  GTGCAG              
BstAPI        184  GCATTAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              784  GCAGCACATGC         
BstF5I        474  GGATG                GGATG(2/0)
             1058  GGATG               
DdeI          337  CTCAG                C^T[ACGT]AG
              437  CTAAG               
FokI          474  GGATG                GGATG(9/13)
             1058  GGATG               
HphI          331  GGTGA                GGTGA(8/7)
              703  TCACC               
MaeI          251  CTAG                 C^TAG
              626  CTAG                
MseI          806  TTAA                 T^TAA
             1031  TTAA                
MspA1I        125  CAGCTG               C[AC]G^C[GT]G
              176  CAGCTG              
NspI          789  ACATGC               [GA]CATG^[CT]
             1106  GCATGT              
PvuII         125  CAGCTG               CAG^CTG
              176  CAGCTG              
TaqI          729  TCGA                 T^CGA
             1134  TCGA                
Tru9I         806  TTAA                 T^TAA
             1031  TTAA                
TthHB8I       729  TCGA                 T^CGA
             1134  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   36 enzymes) #
#########################################

AfaI        AluI        BalI        BbvI        BsiYI       
BslI        BsrI        BstXI       Cfr13I      DpnII       
EaeI        Fnu4HI      HaeIII      HinfI       ItaI        
MaeIII      MboI        MboII       MluNI       MnlI        
MscI        MslI        MwoI        NdeI        NdeII       
NlaIII      NlaIV       PleI        RsaI        Sau3AI      
Sau96I      SfaNI       TseI        Tsp509I     TspRI       
XcmI        
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