Restriction map for pF1KE0653
##################################
# NO CUT ENZYMES (  156 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AviII       AvrII       BamHI       
BanI        BbeI        BbrPI       BbvCI       BciVI       
BclI        BcnI        BfaI        BfrI        BglI        
BlnI        BlpI        BpmI        Bpu1102I    BsaBI       
BsaHI       BsaWI       BseAI       BseRI       BsiWI       
BsmBI       BsmFI       BsmI        Bsp1407I    BspDI       
BspEI       BspLU11I    BspMI       BsrDI       BsrGI       
BssHII      Bst1107I    BstBI       BstEII      BstPI       
BstXI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DraI        DraIII      DrdI        Eam1105I    
EarI        Eco47III    Eco81I      EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FauI        FbaI        
FseI        FspI        HaeII       HgaI        Hin1I       
HincII      HindII      HindIII     HpaI        KasI        
KpnI        Ksp632I     KspI        MaeI        MamI        
MfeI        MluI        MroI        MseI        MunI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PstI        PvuI        RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        TfiI        Tru9I       Tth111I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   60 enzymes) #
###################################

AcsI          505  AAATTC               [GA]^AATT[CT]
AfaI          108  GTAC                 GT^AC
AlwI          384  GGATC                GGATC(4/5)
AlwNI         373  CAGGACCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          505  AAATTC               [GA]^AATT[CT]
AspHI         130  GAGCTC               G[AT]GC[AT]^C
AvaI          185  CCCGAG               C^[CT]CG[GA]G
BalI          231  TGGCCA               TGG^CCA
BanII         130  GAGCTC               G[GA]GC[CT]^C
BbsI          466  GAAGAC               GAAGAC(2/6)
BbvI          365  GCAGC                GCAGC(8/12)
BcgI           65  CGACCGACGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII         322  AGATCT               A^GATCT
BpuAI         466  GAAGAC               GAAGAC(2/6)
BsaAI          49  TACGTG               [CT]AC^GT[GA]
BsaI          581  GGTCTC               GGTCTC(1/5)
BsgI          424  GTGCAG               GTGCAG(16/14)
BsiHKAI       130  GAGCTC               G[AT]GC[AT]^C
BsmAI         582  GTCTC                GTCTC(1/5)
BsoBI         185  CCCGAG               C^[CT]CG[GA]G
BspHI         516  TCATGA               T^CATGA
BsrBI         510  CCGCTC               CCGCTC(-3/-3)
BsrFI         140  ACCGGC               [GA]^CCGG[CT]
BssKI         260  CCAGG                ^CC[ACGT]GG
BssSI         151  CTCGTG               CACGAG(-5/-1)
BstAPI        193  GCAGAGGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI         260  CCAGG                CC^[AT]GG
BstYI         322  AGATCT               [GA]^GATC[CT]
Cac8I         368  GCTGGC               GC[ACGT]^[ACGT]GC
Cfr10I        140  ACCGGC               [GA]^CCGG[CT]
DsaI          291  CCGTGG               C^C[GA][CT]GG
EagI          111  CGGCCG               C^GGCCG
EclXI         111  CGGCCG               C^GGCCG
Eco52I        111  CGGCCG               C^GGCCG
EcoNI         355  CCTCCGTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII        260  CCAGG                ^CC[AT]GG
HapII         141  CCGG                 C^CGG
HinfI         607  GACTC                G^A[ACGT]TC
HpaII         141  CCGG                 C^CGG
HphI          523  GGTGA                GGTGA(8/7)
MaeIII        605  GTGAC                ^GT[ACGT]AC
MflI          322  AGATCT               [GA]^GATC[CT]
MluNI         231  TGGCCA               TGG^CCA
MscI          231  TGGCCA               TGG^CCA
MspA1I        366  CAGCTG               C[AC]G^C[GT]G
MspI          141  CCGG                 C^CGG
MvaI          260  CCAGG                CC^[AT]GG
NspI          389  ACATGC               [GA]CATG^[CT]
PleI          607  GACTC                GAGTC(4/5)
PvuII         366  CAGCTG               CAG^CTG
RcaI          516  TCATGA               T^CATGA
RsaI          108  GTAC                 GT^AC
SacI          130  GAGCTC               GAGCT^C
ScrFI         260  CCAGG                CC^[ACGT]GG
SmlI          133  CTCAAG               C^T[CT][GA]AG
TseI          365  GCAGC                G^C[AT]GC
Tsp45I        605  GTGAC                ^GT[GC]AC
Tsp509I       506  AATT                 ^AATT
TspRI         235  CAGTG                CA[GC]TG[ACGT][ACGT]^
XhoII         322  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

BmyI          130  GAGCTC               G[AGT]GC[ACT]^C
              154  GTGCCC              
BsaJI         259  CCCAGG               C^C[ACGT][ACGT]GG
              291  CCGTGG              
BsiEI          65  CGACCG               CG[GA][CT]^CG
              111  CGGCCG              
Bsp1286I      130  GAGCTC               G[AGT]GC[ACT]^C
              154  GTGCCC              
BsrI          492  ACTGG                ACTGG(1/-1)
              234  CCAGT               
DdeI          457  CTCAG                C^T[ACGT]AG
              551  CTGAG               
EaeI          111  CGGCCG               [CT]^GGCC[GA]
              231  TGGCCA              
MaeII          50  ACGT                 A^CGT
               71  ACGT                
MslI           45  CATCTACGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              517  CATGAGGGTG          


#########################################
# 3 OR MORE CUT ENZYMES (   33 enzymes) #
#########################################

AccII       AciI        AluI        AvaII       BsiYI       
BslI        BstF5I      BstUI       CfoI        Cfr13I      
DpnII       DraII       EcoO109I    Fnu4HI      FokI        
HaeIII      HhaI        HinP1I      ItaI        MboI        
MboII       MnlI        MvnI        MwoI        NdeII       
NlaIII      NlaIV       PpuMI       Sau3AI      Sau96I      
SfaNI       TaqI        TthHB8I     
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