Restriction map for pF1KE0653
 (Linear) (Six-base) MAPSORT of: KIEE0653.seq  Check: 8370  from: 1  to: 663



KIEE0653.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:16  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     400     663 
   Size:        400     263
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      82     114     663 
   Size:         82      32     549

  Fragments arranged by size: 

                 549      82      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     378     663 
   Size:        378     285
 
ApoI r'AATT_y

Cuts at:      0     505     663 
   Size:        505     158
 
AvaI C'yCGr_G

Cuts at:      0     185     663 
   Size:        185     478
 
BanII G_rGCy'C

Cuts at:      0     134     663 
   Size:        134     529
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     473     663 
   Size:        473     190
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      54      88     663 
   Size:         54      34     575

  Fragments arranged by size: 

                 575      54      34
 
BglII A'GATC_T

Cuts at:      0     322     663 
   Size:        322     341
 
Bme1580I G_kGCm'C

Cuts at:      0     158     663 
   Size:        158     505
 
BmgBI CAC'GTC

Cuts at:      0      72     663 
   Size:         72     591
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     118     663 
   Size:        118     545
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     587     663 
   Size:        587      76
 
BsaAI yAC'GTr

Cuts at:      0      51     663 
   Size:         51     612
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      82     112     663 
   Size:         82      30     551

  Fragments arranged by size: 

                 551      82      30
 
BseYI C'CCAG_C

Cuts at:      0       8     400     663 
   Size:          8     392     263

  Fragments arranged by size: 

                 392     263       8
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     445     663 
   Size:        445     218
 
BsiEI CG_ry'CG

Cuts at:      0      68     114     663 
   Size:         68      46     549

  Fragments arranged by size: 

                 549      68      46
 
BsiHKAI G_wGCw'C

Cuts at:      0     134     663 
   Size:        134     529
 
Bsp1286I G_dGCh'C

Cuts at:      0     134     158     663 
   Size:        134      24     505

  Fragments arranged by size: 

                 505     134      24
 
BspHI T'CATG_A

Cuts at:      0     516     663 
   Size:        516     147
 
BsrBI CCG'CTC

Cuts at:      0     512     663 
   Size:        512     151
 
BsrFI r'CCGG_y

Cuts at:      0     140     663 
   Size:        140     523
 
BssSI C'ACGA_G

Cuts at:      0     151     663 
   Size:        151     512
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     199     663 
   Size:        199     464
 
BstYI r'GATC_y

Cuts at:      0     322     663 
   Size:        322     341
 
BtgI C'CryG_G

Cuts at:      0     291     663 
   Size:        291     372
 
EaeI y'GGCC_r

Cuts at:      0     111     231     663 
   Size:        111     120     432

  Fragments arranged by size: 

                 432     120     111
 
EagI C'GGCC_G

Cuts at:      0     111     663 
   Size:        111     552
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      28     663 
   Size:         28     635
 
EcoICRI GAG'CTC

Cuts at:      0     132     663 
   Size:        132     531
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     400     663 
   Size:        400     263
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     359     663 
   Size:        359     304
 
EcoO109I rG'GnC_Cy

Cuts at:      0      93     180     375     656     663 
   Size:         93      87     195     281       7

  Fragments arranged by size: 

                 281     195      93      87       7
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      88     663 
   Size:         88     575
 
MscI TGG'CCA

Cuts at:      0     233     663 
   Size:        233     430
 
MslI CAynn'nnrTG

Cuts at:      0      49     521     663 
   Size:         49     472     142

  Fragments arranged by size: 

                 472     142      49
 
MspA1I CmG'CkG

Cuts at:      0     368     663 
   Size:        368     295
 
NspI r_CATG'y

Cuts at:      0     393     663 
   Size:        393     270
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      82     114     663 
   Size:         82      32     549

  Fragments arranged by size: 

                 549      82      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      93     180     375     663 
   Size:         93      87     195     288

  Fragments arranged by size: 

                 288     195      93      87
 
PvuII CAG'CTG

Cuts at:      0     368     663 
   Size:        368     295
 
SacI G_AGCT'C

Cuts at:      0     134     663 
   Size:        134     529
 
SmlI C'TyrA_G

Cuts at:      0     133     663 
   Size:        133     530
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      82     272     575     663 
   Size:         82     190     303      88

  Fragments arranged by size: 

                 303     190      88      82
 

 Enzymes that do cut:

     AcuI     AloI    AlwNI     ApoI     AvaI    BanII     BbsI     BcgI
    BglII Bme1580I    BmgBI    BpuEI     BsaI    BsaAI    BsaXI    BseYI
     BsgI    BsiEI  BsiHKAI Bsp1286I    BspHI    BsrBI    BsrFI    BssSI
   BstAPI    BstYI     BtgI     EaeI     EagI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I     MmeI     MscI     MslI   MspA1I     NspI     PpiI
    PpuMI    PvuII     SacI     SmlI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI    BciVI
     BclI    BfrBI     BglI     BlpI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BsaBI    BsaHI    BsaWI    BseRI    BsiWI     BsmI    BsmBI
    BspEI    BspMI    BsrDI    BsrGI   BssHII    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EarI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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