Restriction map for pF1KE0639
 (Circular) (Six-base) MAPSORT of: pF1KE0639.seq  Check: 6580  from: 1  to: 3755



pF1KE0639  3755 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:16  ..

AccI GT'mk_AC

Cuts at:    793     793 
   Size:       3755
 
Acc65I G'GTAC_C

Cuts at:    771     771 
   Size:       3755
 
AclI AA'CG_TT

Cuts at:   3595    3595 
   Size:       3755
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    541    1580    2012    2759    3027     541 
   Size:       1039     432     747     268    1269

  Fragments arranged by size: 

                1269    1039     747     432     268
 
AflIII A'CryG_T

Cuts at:    596    1107    2211    3188     596 
   Size:        511    1104     977    1163

  Fragments arranged by size: 

                1163    1104     977     511
 
AgeI A'CCGG_T

Cuts at:   2143    2143 
   Size:       3755
 
AhdI GACnn_n'nnGTC

Cuts at:    696     696 
   Size:       3755
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    448     448 
   Size:       3755
 
AlwNI CAG_nnn'CTG

Cuts at:   1060    2627    1060 
   Size:       1567    2188
 
ApaI G_GGCC'C

Cuts at:    119     119 
   Size:       3755
 
ApaLI G'TGCA_C

Cuts at:   2525    2525 
   Size:       3755
 
ApoI r'AATT_y

Cuts at:    759    3108    3364     759 
   Size:       2349     256    1150

  Fragments arranged by size: 

                2349    1150     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3755
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3755
 
AvaI C'yCGr_G

Cuts at:    652     775     652 
   Size:        123    3632
 
BamHI G'GATC_C

Cuts at:    780     780 
   Size:       3755
 
BanI G'GyrC_C

Cuts at:    771    1185    1433    1468     771 
   Size:        414     248      35    3058

  Fragments arranged by size: 

                3058     414     248      35
 
BanII G_rGCy'C

Cuts at:    119     547     769    1799     119 
   Size:        428     222    1030    2075

  Fragments arranged by size: 

                2075    1030     428     222
 
BbeI G_GCGC'C

Cuts at:   1189    1437    1189 
   Size:        248    3507
 
BbsI GAAGACnn'nnnn_

Cuts at:    447     447 
   Size:       3755
 
BciVI GTATCCnnnnn_n'

Cuts at:   1653    2414    3755    1653 
   Size:        761    1341    1653

  Fragments arranged by size: 

                1653    1341     761
 
BclI T'GATC_A

Cuts at:   1275    1275 
   Size:       3755
 
BglI GCCn_nnn'nGGC

Cuts at:    143     143 
   Size:       3755
 
BglII A'GATC_T

Cuts at:   1270    3307    1270 
   Size:       2037    1718
 
BlpI GC'TnA_GC

Cuts at:    867     867 
   Size:       3755
 
Bme1580I G_kGCm'C

Cuts at:    119    1380    1473    2529     119 
   Size:       1261      93    1056    1345

  Fragments arranged by size: 

                1345    1261    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    967    1048    1383    3527     967 
   Size:         81     335    2144    1195

  Fragments arranged by size: 

                2144    1195     335      81
 
BmtI G_CTAG'C

Cuts at:    476     476 
   Size:       3755
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2122    2122 
   Size:       3755
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    289     931    1810    2302    2600     289 
   Size:        642     879     492     298    1444

  Fragments arranged by size: 

                1444     879     642     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    718    3459     718 
   Size:       2741    1014
 
BsaAI yAC'GTr

Cuts at:    179     621    1108     179 
   Size:        442     487    2826

  Fragments arranged by size: 

                2826     487     442
 
BsaBI GATnn'nnATC

Cuts at:      6     298    1269    3306       6 
   Size:        292     971    2037     455

  Fragments arranged by size: 

                2037     971     455     292
 
BsaHI Gr'CG_yC

Cuts at:    680    1186    1434    2136    3632     680 
   Size:        506     248     702    1496     803

  Fragments arranged by size: 

                1496     803     702     506     248
 
BsaWI w'CCGG_w

Cuts at:    944    1465    2143    2417    2564    2975     944 
   Size:        521     678     274     147     411    1724

  Fragments arranged by size: 

                1724     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    274     274 
   Size:       3755
 
BseYI C'CCAG_C

Cuts at:    122    1180    1938    2515    3011     122 
   Size:       1058     758     577     496     866

  Fragments arranged by size: 

                1058     866     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    352     352 
   Size:       3755
 
BsiEI CG_ry'CG

Cuts at:    104    1297    1343    2551     104 
   Size:       1193      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1193      46
 
BsiHKAI G_wGCw'C

Cuts at:    547     626     769    1547    1737    2529     547 
   Size:         79     143     778     190     792    1773

  Fragments arranged by size: 

                1773     792     778     190     143      79
 
BsiWI C'GTAC_G

Cuts at:    179     179 
   Size:       3755
 
BsmI GAATG_Cn'

Cuts at:    311     311 
   Size:       3755
 
Bsp1286I G_dGCh'C

Cuts at:    119     547     626     769    1380    1473    1547    1737    1799 
   Size:        428      79     143     611      93      74     190      62

Cuts at:   1799    2529     119 
   Size:        730    1345

  Fragments arranged by size: 

                1345     730     611     428     190     143      93      79
                  74      62
 
BspEI T'CCGG_A

Cuts at:   2975    2975 
   Size:       3755
 
BspHI T'CATG_A

Cuts at:   2931    2931 
   Size:       3755
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    805    1019    1321    1702     805 
   Size:        214     302     381    2858

  Fragments arranged by size: 

                2858     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2047    2101    3585    3750      50 
   Size:       1997      54    1484     165      55

  Fragments arranged by size: 

                1997    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1667    1667 
   Size:       3755
 
BsrFI r'CCGG_y

Cuts at:   1753    1934    2143    1753 
   Size:        181     209    3365

  Fragments arranged by size: 

                3365     209     181
 
BsrGI T'GTAC_A

Cuts at:    191     191 
   Size:       3755
 
BssSI C'ACGA_G

Cuts at:    218    2026    2384     218 
   Size:       1808     358    1589

  Fragments arranged by size: 

                1808    1589     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:    489    1132     489 
   Size:        643    3112
 
BstBI TT'CG_AA

Cuts at:   2116    2116 
   Size:       3755
 
BstYI r'GATC_y

Cuts at:      1     299     516     780    1247    1270    1605    1851    2863 
   Size:        298     217     264     467      23     335     246    1012

Cuts at:   2863    2949    2961    3307       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1012     467     449     346     335     298     264     246
                 217      86      23      12
 
BtgI C'CryG_G

Cuts at:    107     185     604     627     701     107 
   Size:         78     419      23      74    3161

  Fragments arranged by size: 

                3161     419      78      74      23
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1705    1886    2049    1705 
   Size:        181     163    3411

  Fragments arranged by size: 

                3411     181     163
 
BtsI GCAGTG_nn'

Cuts at:    998     998 
   Size:       3755
 
DraI TTT'AAA

Cuts at:    754     754 
   Size:       3755
 
DraIII CAC_nnn'GTG

Cuts at:    330     330 
   Size:       3755
 
DrdI GACnn_nn'nnGTC

Cuts at:   1461    2319    1461 
   Size:        858    2897
 
EaeI y'GGCC_r

Cuts at:   1340    1514    1905    1932    3277    1340 
   Size:        174     391      27    1345    1818

  Fragments arranged by size: 

                1818    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1340    1340 
   Size:       3755
 
EarI CTCTTCn'nnn_

Cuts at:   1778    1988    1778 
   Size:        210    3545
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1963    2273    2419    3565    1963 
   Size:        310     146    1146    2153

  Fragments arranged by size: 

                2153    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    545     767     545 
   Size:        222    3533
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    541    1580    2012    2759    3027     541 
   Size:       1039     432     747     268    1269

  Fragments arranged by size: 

                1269    1039     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    115     127     894     115 
   Size:         12     767    2976

  Fragments arranged by size: 

                2976     767      12
 
EcoRI G'AATT_C

Cuts at:    759     759 
   Size:       3755
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1774    1774 
   Size:       3755
 
FspI TGC'GCA

Cuts at:    390    1536     390 
   Size:       1146    2609
 
HaeII r_GCGC'y

Cuts at:    165     479    1189    1437    2459    3438     165 
   Size:        314     710     248    1022     979     482

  Fragments arranged by size: 

                1022     979     710     482     314     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    682     714    1597    1627    1629    1659     682 
   Size:         32     883      30       2      30    2778

  Fragments arranged by size: 

                2778     883      32      30      30       2
 
HincII GTy'rAC

Cuts at:    794     794 
   Size:       3755
 
KasI G'GCGC_C

Cuts at:   1185    1433    1185 
   Size:        248    3507
 
KpnI G_GTAC'C

Cuts at:    775     775 
   Size:       3755
 
MluI A'CGCG_T

Cuts at:   3188    3188 
   Size:       3755
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2426    2610    2426 
   Size:        184    3571
 
MscI TGG'CCA

Cuts at:   1516    1516 
   Size:       3755
 
MslI CAynn'nnrTG

Cuts at:   1744    1871    1744 
   Size:        127    3628
 
MspA1I CmG'CkG

Cuts at:    173     336     669     866    1180    1540    2553    2798     173 
   Size:        163     333     197     314     360    1013     245    1130

  Fragments arranged by size: 

                1130    1013     360     333     314     245     197     163
            
 
NaeI GCC'GGC

Cuts at:   1936    1936 
   Size:       3755
 
NarI GG'CG_CC

Cuts at:   1186    1434    1186 
   Size:        248    3507
 
NcoI C'CATG_G

Cuts at:    107     701     107 
   Size:        594    3161
 
NgoMIV G'CCGG_C

Cuts at:   1934    1934 
   Size:       3755
 
NheI G'CTAG_C

Cuts at:    472     472 
   Size:       3755
 
NspI r_CATG'y

Cuts at:    600     808    1308    2215     600 
   Size:        208     500     907    2140

  Fragments arranged by size: 

                2140     907     500     208
 
PciI A'CATG_T

Cuts at:    596    2211     596 
   Size:       1615    2140
 
PmeI GTTT'AAAC

Cuts at:    754     754 
   Size:       3755
 
PmlI CAC'GTG

Cuts at:    621     621 
   Size:       3755
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2894    2926    2894 
   Size:         32    3723
 
PpuMI rG'GwC_Cy

Cuts at:    127     127 
   Size:       3755
 
PspOMI G'GGCC_C

Cuts at:    115     115 
   Size:       3755
 
PstI C_TGCA'G

Cuts at:    802    1487     802 
   Size:        685    3070
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3755
 
PvuII CAG'CTG

Cuts at:    336    1180    1540     336 
   Size:        844     360    2551

  Fragments arranged by size: 

                2551     844     360
 
RsrII CG'GwC_CG

Cuts at:   1950    1950 
   Size:       3755
 
SacI G_AGCT'C

Cuts at:    547     769     547 
   Size:        222    3533
 
SalI G'TCGA_C

Cuts at:    792     792 
   Size:       3755
 
SanDI GG'GwC_CC

Cuts at:    127     127 
   Size:       3755
 
SapI GCTCTTCn'nnn_

Cuts at:   1778    1988    1778 
   Size:        210    3545
 
SbfI CC_TGCA'GG

Cuts at:    802     802 
   Size:       3755
 
ScaI AGT'ACT

Cuts at:   3272    3272 
   Size:       3755
 
SfcI C'TryA_G

Cuts at:     33     798    1483    2476    2667      33 
   Size:        765     685     993     191    1121

  Fragments arranged by size: 

                1121     993     765     685     191
 
SfoI GGC'GCC

Cuts at:   1187    1435    1187 
   Size:        248    3507
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3755
 
SmaI CCC'GGG

Cuts at:    777     777 
   Size:       3755
 
SmlI C'TyrA_G

Cuts at:    304     910    1825    2317    2579     304 
   Size:        606     915     492     262    1480

  Fragments arranged by size: 

                1480     915     606     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3755
 
SphI G_CATG'C

Cuts at:    808    1308     808 
   Size:        500    3255
 
StuI AGG'CCT

Cuts at:    199     199 
   Size:       3755
 
StyI C'CwwG_G

Cuts at:    107     548     701     889    1243     107 
   Size:        441     153     188     354    2619

  Fragments arranged by size: 

                2619     441     354     188     153
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1338    1471    1930    2139    1338 
   Size:        133     459     209    2954

  Fragments arranged by size: 

                2954     459     209     133
 
TatI w'GTAC_w

Cuts at:    191    3270     191 
   Size:       3079     676
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3272    3304    3272 
   Size:         32    3723
 
Tth111I GACn'n_nGTC

Cuts at:   1552    1552 
   Size:       3755
 
XbaI T'CTAG_A

Cuts at:    786     786 
   Size:       3755
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1250    1250 
   Size:       3755
 
XmaI C'CCGG_G

Cuts at:    775     775 
   Size:       3755
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AhdI     AlfI
    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I     BmrI     BmtI     BplI    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI    BssSI   BstAPI    BstBI    BstYI     BtgI    BtgZI
     BtsI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NheI     NspI     PciI     PmeI
     PmlI     PpiI    PpuMI   PspOMI     PstI     PvuI    PvuII    RsrII
     SacI     SalI    SanDI     SapI     SbfI     ScaI     SfcI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AleI     AloI     AscI    AvrII
     BaeI    BbvCI     BcgI    BfrBI    BmgBI     BpmI   Bpu10I    BsaXI
    BsmBI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     ClaI    CspCI
    EcoNI    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI     NdeI
     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI    PshAI
     PsiI    PspXI     PsrI    SacII    SexAI     SfiI    SgrAI    SnaBI
     SrfI     SspI     SwaI     XhoI     XmnI     ZraI
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