Restriction map for pF1KE0618
##################################
# NO CUT ENZYMES (  149 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AflIII      AgeI        Alw44I      
Aor51HI     ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspHI       AspI        AvrII       
BamHI       BanI        BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BcnI        BfrI        
BglI        BlnI        BlpI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BsaWI       BseAI       BsiEI       
BsiHKAI     BsiWI       BsmBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstXI       
BstZ17I     CelII       Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        EagI        EclXI       
Eco47III    Eco52I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        HaeII       HapII       
Hin1I       HindIII     HpaI        HpaII       KasI        
KpnI        KspI        MamI        MfeI        MluI        
MroI        MseI        MspI        MunI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tru9I       Tth111I     Van91I      XbaI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccII         739  CGCG                 CG^CG
AcsI          146  AAATTC               [GA]^AATT[CT]
AhdI          744  GACTCGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI          345  GATCC                GGATC(4/5)
AocI          525  CCTGAGG              CC^T[ACGT]AGG
ApaI          359  GGGCCC               GGGCC^C
ApoI          146  AAATTC               [GA]^AATT[CT]
AspEI         744  GACTCGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI          356  CTCGGG               C^[CT]CG[GA]G
AviII         884  TGCGCA               TGC^GCA
BalI          860  TGGCCA               TGG^CCA
BbsI          615  GAAGAC               GAAGAC(2/6)
BfaI          892  CTAG                 C^TAG
BglII         225  AGATCT               A^GATCT
BpuAI         615  GAAGAC               GAAGAC(2/6)
BsaI          542  GAGACC               GGTCTC(1/5)
BsmI          532  GAATGC               GAATGC(1/-1)
BsoBI         356  CTCGGG               C^[CT]CG[GA]G
BstAPI        876  GCAGTCTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         739  CGCG                 CG^CG
BstYI         225  AGATCT               [GA]^GATC[CT]
Bsu36I        525  CCTGAGG              CC^T[ACGT]AGG
DsaI          576  CCGTGG               C^C[GA][CT]GG
EaeI          860  TGGCCA               [CT]^GGCC[GA]
Eam1105I      744  GACTCGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          280  CTCTTC               CTCTTC(1/4)
Eco81I        525  CCTGAGG              CC^T[ACGT]AGG
EcoT14I      1070  CCTTGG               C^C[AT][AT]GG
FspI          884  TGCGCA               TGC^GCA
HgaI          572  GCGTC                GACGC(5/10)
HincII        442  GTTGAC               GT[CT]^[GA]AC
HindII        442  GTTGAC               GT[CT]^[GA]AC
Ksp632I       280  CTCTTC               CTCTTC(1/4)
MaeI          892  CTAG                 C^TAG
MaeII         641  ACGT                 A^CGT
MflI          225  AGATCT               [GA]^GATC[CT]
MluNI         860  TGGCCA               TGG^CCA
MscI          860  TGGCCA               TGG^CCA
MspA1I        364  CCGCTG               C[AC]G^C[GT]G
MvnI          739  CGCG                 CG^CG
NspI          665  ACATGC               [GA]CATG^[CT]
PstI         1081  CTGCAG               CTGCA^G
SfaNI        1036  GCATC                GCATC(5/9)
SfcI         1081  CTGCAG               C^T[GA][CT]AG
StyI         1070  CCTTGG               C^C[AT][AT]GG
TaqI          981  TCGA                 T^CGA
Tsp509I       147  AATT                 ^AATT
TthHB8I       981  TCGA                 T^CGA
XhoII         225  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AfaI          153  GTAC                 GT^AC
              762  GTAC                
BseRI          64  GAGGAG               GAGGAG(10/8)
              700  GAGGAG              
BsgI          655  CTGCAC               GTGCAG(16/14)
              874  GTGCAG              
BsrI          126  ACTGG                ACTGG(1/-1)
             1003  ACTGG               
CfoI          738  GCGC                 GCG^C
              885  GCGC                
DpnII         226  GATC                 ^GATC
              345  GATC                
HhaI          738  GCGC                 GCG^C
              885  GCGC                
HinP1I        738  GCGC                 G^CGC
              885  GCGC                
MboI          226  GATC                 ^GATC
              345  GATC                
MslI          729  CACCGTTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              867  CATCCCTGTG          
NdeII         226  GATC                 ^GATC
              345  GATC                
PleI          744  GACTC                GAGTC(4/5)
              927  GACTC               
PpuMI         331  AGGACCC              [GA]G^G[AT]CC[CT]
              505  GGGTCCT             
RsaI          153  GTAC                 GT^AC
              762  GTAC                
Sau3AI        226  GATC                 ^GATC
              345  GATC                
TfiI           83  GATTC                G^A[AT]TC
              983  GAATC               
XcmI          261  CCAGGAGCTGAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              567  CCATAGCGTCCGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   43 enzymes) #
#########################################

AciI        AluI        AlwNI       AvaII       BanII       
BbvI        BmyI        BpmI        BsaJI       BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BssKI       
BstF5I      BstNI       Cac8I       Cfr13I      DdeI        
DraII       EcoNI       EcoO109I    EcoRII      FauI        
Fnu4HI      FokI        HaeIII      HinfI       HphI        
ItaI        MaeIII      MboII       MnlI        MvaI        
MwoI        NlaIII      NlaIV       Sau96I      ScrFI       
TseI        Tsp45I      TspRI       
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