Restriction map for pF1KE0617
##################################
# NO CUT ENZYMES (  188 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AgeI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspHI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BcnI        BfrI        
BglI        BglII       BlnI        BlpI        BmyI        
BpmI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmI        BsoBI       Bsp1286I    
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrDI       BsrFI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      Cac8I       
CelII       CfoI        Cfr10I      ClaI        CpoI        
DdeI        DraI        DraII       DraIII      DrdI        
EagI        EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HaeII       HapII       HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HinfI       HpaI        
HpaII       HphI        KasI        KpnI        Ksp632I     
KspI        MaeIII      MamI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MseI        
MslI        MspI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NlaIII      NlaIV       NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspI        StuI        SwaI        TfiI        Tru9I       
Tsp45I      TspRI       Tth111I     Van91I      XhoI        
XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccI          440  GTAGAC               GT^[AC][GT]AC
AcsI           85  GAATTT               [GA]^AATT[CT]
AflIII         26  ACACGT               A^C[GA][CT]GT
AhdI           58  GACACCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AluI          502  AGCT                 AG^CT
AlwI          264  GGATC                GGATC(4/5)
ApoI           85  GAATTT               [GA]^AATT[CT]
Asp700I       470  GAAGCCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI          58  GACACCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII         100  GGTCC                G^G[AT]CC
BbvI          500  GCAGC                GCAGC(8/12)
BseRI         378  GAGGAG               GAGGAG(10/8)
BsiYI         293  CCAAGGAAGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          293  CCAAGGAAGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI          30  GTCTC                GTCTC(1/5)
BsmBI          29  CGTCTC               CGTCTC(1/5)
Bsp1407I      311  TGTACA               T^GTACA
BspMI          11  ACCTGC               ACCTGC(4/8)
BsrGI         311  TGTACA               T^GTACA
BstF5I        177  GGATG                GGATG(2/0)
Cfr13I        100  GGTCC                G^G[ACGT]CC
DsaI          457  CCACGG               C^C[GA][CT]GG
EaeI           50  TGGCCG               [CT]^GGCC[GA]
Eam1105I       58  GACACCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I       293  CCAAGG               C^C[AT][AT]GG
FauI            5  CCCGC                CCCGC(4/6)
FokI          177  GGATG                GGATG(9/13)
HaeIII         51  GGCC                 GG^CC
MspA1I        501  CAGCTG               C[AC]G^C[GT]G
MwoI          190  GCTGAAGACGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PstI          498  CTGCAG               CTGCA^G
PvuII         501  CAGCTG               CAG^CTG
Sau96I        100  GGTCC                G^G[ACGT]CC
SexAI          61  ACCAGGT              A^CC[AT]GGT
SfaNI         187  GATGC                GCATC(5/9)
SspBI         311  TGTACA               T^GTACA
StyI          293  CCAAGG               C^C[AT][AT]GG
TaqI          267  TCGA                 T^CGA
TseI          500  GCAGC                G^C[AT]GC
TthHB8I       267  TCGA                 T^CGA
XbaI          355  TCTAGA               T^CTAGA
XcmI          493  CCAAACTGCAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          470  GAAGCCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AciI            6  CCGC                 CCGC(-3/-1)
               78  GCGG                
AfaI          312  GTAC                 GT^AC
              516  GTAC                
BfaI          356  CTAG                 C^TAG
              431  CTAG                
BsmFI           3  GTCCC                GGGAC(10/14)
               17  GTCCC               
BsrI          448  ACTGG                ACTGG(1/-1)
              513  CCAGT               
BssKI          62  CCAGG                ^CC[ACGT]GG
              258  CCAGG               
BstNI          62  CCAGG                CC^[AT]GG
              258  CCAGG               
EcoRII         62  CCAGG                ^CC[AT]GG
              258  CCAGG               
Fnu4HI         78  GCGGC                GC^[ACGT]GC
              500  GCAGC               
HgaI          196  GACGC                GACGC(5/10)
               15  GCGTC               
ItaI           78  GCGGC                GC^[ACGT]GC
              500  GCAGC               
MaeI          356  CTAG                 C^TAG
              431  CTAG                
MvaI           62  CCAGG                CC^[AT]GG
              258  CCAGG               
RsaI          312  GTAC                 GT^AC
              516  GTAC                
ScrFI          62  CCAGG                CC^[ACGT]GG
              258  CCAGG               


#########################################
# 3 OR MORE CUT ENZYMES (   12 enzymes) #
#########################################

BbsI        BpuAI       BsaJI       DpnII       MaeII       
MboI        MboII       MnlI        NdeII       Sau3AI      
SfcI        Tsp509I     
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com