Restriction map for pF1KE0615
##################################
# NO CUT ENZYMES (  163 enzymes) #
##################################

Acc65I      AccIII      AclI        AfaI        AflII       
AflIII      AgeI        AhdI        AluI        Alw44I      
AlwI        AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AviII       
AvrII       BamHI       BanI        BanII       BbeI        
BbrPI       BbvCI       BcgI        BciVI       BclI        
BfrI        BglI        BlnI        BlpI        BmyI        
Bpu1102I    BsaAI       BsaBI       BsaI        BseAI       
BsgI        BsiEI       BsiHKAI     BsiWI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BstAPI      BstBI       BstEII      BstPI       
BstXI       Bsu36I      Cac8I       CelII       Cfr10I      
ClaI        CpoI        DdeI        DraI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HindIII     HpaI        HphI        KasI        
KpnI        KspI        MamI        MfeI        MluI        
MroI        MslI        MspA1I      MunI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        Tsp45I      TspRI       
Tth111I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AatII         515  GACGTC               GACGT^C
AccI          345  GTATAC               GT^[AC][GT]AC
AcsI          274  AAATTC               [GA]^AATT[CT]
AcyI          515  GACGTC               G[GA]^CG[CT]C
ApoI          274  AAATTC               [GA]^AATT[CT]
AvaI          137  CCCGAG               C^[CT]CG[GA]G
BalI          405  TGGCCA               TGG^CCA
BcnI          190  CCCGG                CC^[GC]GG
BglII          15  AGATCT               A^GATCT
BpmI          233  CTGGAG               CTGGAG(16/14)
BsaHI         515  GACGTC               G[GA]^CG[CT]C
BsaWI         509  ACCGGA               [AT]^CCGG[AT]
BseRI         474  GAGGAG               GAGGAG(10/8)
BsmAI         513  GAGAC                GTCTC(1/5)
BsmBI         513  GAGACG               CGTCTC(1/5)
BsmI           71  GCATTC               GAATGC(1/-1)
BsoBI         137  CCCGAG               C^[CT]CG[GA]G
BssSI          22  CACGAG               CACGAG(-5/-1)
Bst1107I      345  GTATAC               GTA^TAC
BstYI          15  AGATCT               [GA]^GATC[CT]
BstZ17I       345  GTATAC               GTA^TAC
CfoI          142  GCGC                 GCG^C
DpnII          16  GATC                 ^GATC
DraII         133  GGGTCCC              [GA]G^G[ACGT]CC[CT]
DsaI           47  CCATGG               C^C[GA][CT]GG
EarI          127  GAAGAG               CTCTTC(1/4)
EcoO109I      133  GGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoRV         332  GATATC               GAT^ATC
EcoT14I        47  CCATGG               C^C[AT][AT]GG
HgaI          491  GACGC                GACGC(5/10)
HhaI          142  GCGC                 GCG^C
Hin1I         515  GACGTC               G[GA]^CG[CT]C
HinP1I        142  GCGC                 G^CGC
HincII        436  GTTGAC               GT[CT]^[GA]AC
HindII        436  GTTGAC               GT[CT]^[GA]AC
Ksp632I       127  GAAGAG               CTCTTC(1/4)
MaeIII        229  GTAAC                ^GT[ACGT]AC
MboI           16  GATC                 ^GATC
MflI           15  AGATCT               [GA]^GATC[CT]
MluNI         405  TGGCCA               TGG^CCA
MscI          405  TGGCCA               TGG^CCA
MseI          296  TTAA                 T^TAA
NciI          190  CCCGG                CC^[GC]GG
NcoI           47  CCATGG               C^CATGG
NdeII          16  GATC                 ^GATC
PpuMI         133  GGGTCCC              [GA]G^G[AT]CC[CT]
Sau3AI         16  GATC                 ^GATC
SfaNI         147  GATGC                GCATC(5/9)
StyI           47  CCATGG               C^C[AT][AT]GG
TaqI          317  TCGA                 T^CGA
TfiI          219  GATTC                G^A[AT]TC
Tru9I         296  TTAA                 T^TAA
TthHB8I       317  TCGA                 T^CGA
XhoII          15  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AccII         112  CGCG                 CG^CG
              143  CGCG                
BbsI          259  GAAGAC               GAAGAC(2/6)
              488  GAAGAC              
BbvI          494  GCAGC                GCAGC(8/12)
              385  GCTGC               
BfaI           13  CTAG                 C^TAG
              352  CTAG                
BpuAI         259  GAAGAC               GAAGAC(2/6)
              488  GAAGAC              
BsaJI          47  CCATGG               C^C[ACGT][ACGT]GG
              380  CCCAGG              
BsrI          232  ACTGG                ACTGG(1/-1)
              440  ACTGG               
BstNI         381  CCAGG                CC^[AT]GG
              408  CCAGG               
BstUI         112  CGCG                 CG^CG
              143  CGCG                
EaeI          405  TGGCCA               [CT]^GGCC[GA]
              483  TGGCCG              
EcoRII        381  CCAGG                ^CC[AT]GG
              408  CCAGG               
HaeIII        406  GGCC                 GG^CC
              484  GGCC                
HapII         191  CCGG                 C^CGG
              510  CCGG                
HpaII         191  CCGG                 C^CGG
              510  CCGG                
MaeI           13  CTAG                 C^TAG
              352  CTAG                
MaeII           8  ACGT                 A^CGT
              516  ACGT                
MspI          191  CCGG                 C^CGG
              510  CCGG                
MvaI          381  CCAGG                CC^[AT]GG
              408  CCAGG               
MvnI          112  CGCG                 CG^CG
              143  CGCG                
NlaIII         48  CATG                 CATG^
              241  CATG                
PleI          398  GACTC                GAGTC(4/5)
              236  GAGTC               
TseI          385  GCTGC                G^C[AT]GC
              494  GCAGC               
Tsp509I       275  AATT                 ^AATT
              294  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   18 enzymes) #
#########################################

AciI        AvaII       BsiYI       BslI        BsmFI       
BssKI       BstF5I      Cfr13I      Fnu4HI      FokI        
HinfI       ItaI        MboII       MnlI        MwoI        
NlaIV       Sau96I      ScrFI       
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