Restriction map for pF1KE0614
 (Circular) (Six-base) MAPSORT of: pF1KE0614.seq  Check: 6972  from: 1  to: 4205



pF1KE0614  4205 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:16  ..

AccI GT'mk_AC

Cuts at:   1243    1243 
   Size:       4205
 
Acc65I G'GTAC_C

Cuts at:   1221    1221 
   Size:       4205
 
AclI AA'CG_TT

Cuts at:   4045    4045 
   Size:       4205
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    898    2030    2462    3209    3477     898 
   Size:       1132     432     747     268    1626

  Fragments arranged by size: 

                1626    1132     747     432     268
 
AflIII A'CryG_T

Cuts at:    950    1557    2661    3638     950 
   Size:        607    1104     977    1517

  Fragments arranged by size: 

                1517    1104     977     607
 
AgeI A'CCGG_T

Cuts at:   2593    2593 
   Size:       4205
 
AlwNI CAG_nnn'CTG

Cuts at:   1510    3077    1510 
   Size:       1567    2638
 
ApaLI G'TGCA_C

Cuts at:   2975    2975 
   Size:       4205
 
ApoI r'AATT_y

Cuts at:    988    1009    1209    3558    3814     988 
   Size:         21     200    2349     256    1379

  Fragments arranged by size: 

                2349    1379     256     200      21
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4205
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4205
 
AvaI C'yCGr_G

Cuts at:   1225    1225 
   Size:       4205
 
BamHI G'GATC_C

Cuts at:   1230    1230 
   Size:       4205
 
BanI G'GyrC_C

Cuts at:    702    1221    1635    1883    1918     702 
   Size:        519     414     248      35    2989

  Fragments arranged by size: 

                2989     519     414     248      35
 
BanII G_rGCy'C

Cuts at:   1219    2249    1219 
   Size:       1030    3175
 
BbeI G_GCGC'C

Cuts at:   1639    1887    1639 
   Size:        248    3957
 
BbsI GAAGACnn'nnnn_

Cuts at:    723     865     886     723 
   Size:        142      21    4042

  Fragments arranged by size: 

                4042     142      21
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    178     212     178 
   Size:         34    4171
 
BciVI GTATCCnnnnn_n'

Cuts at:    852    2103    2864    4205     852 
   Size:       1251     761    1341     852

  Fragments arranged by size: 

                1341    1251     852     761
 
BclI T'GATC_A

Cuts at:   1725    1725 
   Size:       4205
 
BglII A'GATC_T

Cuts at:   1720    3757    1720 
   Size:       2037    2168
 
BlpI GC'TnA_GC

Cuts at:   1317    1317 
   Size:       4205
 
Bme1580I G_kGCm'C

Cuts at:   1830    1923    2979    1830 
   Size:         93    1056    3056

  Fragments arranged by size: 

                3056    1056      93
 
BmgBI CAC'GTC

Cuts at:    951     972     951 
   Size:         21    4184
 
BmrI ACTGGGnnnn_n'

Cuts at:    144     647    1417    1498    1833    3977     144 
   Size:        503     770      81     335    2144     372

  Fragments arranged by size: 

                2144     770     503     372     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    842    2572     842 
   Size:       1730    2475
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1190    1190 
   Size:       4205
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1381    2260    2752    3050    1381 
   Size:        879     492     298    2536

  Fragments arranged by size: 

                2536     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3909    3909 
   Size:       4205
 
BsaAI yAC'GTr

Cuts at:   1558    1558 
   Size:       4205
 
BsaBI GATnn'nnATC

Cuts at:      6    1719    3756       6 
   Size:       1713    2037     455

  Fragments arranged by size: 

                2037    1713     455
 
BsaHI Gr'CG_yC

Cuts at:   1636    1884    2586    4082    1636 
   Size:        248     702    1496    1759

  Fragments arranged by size: 

                1759    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1394    1915    2593    2867    3014    3425    1394 
   Size:        521     678     274     147     411    2174

  Fragments arranged by size: 

                2174     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:    400    1630    2388    2965    3461     400 
   Size:       1230     758     577     496    1144

  Fragments arranged by size: 

                1230    1144     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1747    1793    3001     104 
   Size:       1643      46    1208    1308

  Fragments arranged by size: 

                1643    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   1219    1997    2187    2979    1219 
   Size:        778     190     792    2445

  Fragments arranged by size: 

                2445     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:   1219    1830    1923    1997    2187    2249    2979    1219 
   Size:        611      93      74     190      62     730    2445

  Fragments arranged by size: 

                2445     730     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:   3425    3425 
   Size:       4205
 
BspHI T'CATG_A

Cuts at:   3381    3381 
   Size:       4205
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1255    1469    1771    2152    1255 
   Size:        214     302     381    3308

  Fragments arranged by size: 

                3308     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1136    2497    2551    4035    4200      50 
   Size:       1086    1361      54    1484     165      55

  Fragments arranged by size: 

                1484    1361    1086     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    194    1188    2117     194 
   Size:        994     929    2282

  Fragments arranged by size: 

                2282     994     929
 
BsrFI r'CCGG_y

Cuts at:    590    1111    2203    2384    2593     590 
   Size:        521    1092     181     209    2202

  Fragments arranged by size: 

                2202    1092     521     209     181
 
BssSI C'ACGA_G

Cuts at:   2476    2834    2476 
   Size:        358    3847
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1582    1582 
   Size:       4205
 
BstBI TT'CG_AA

Cuts at:   2566    2566 
   Size:       4205
 
BstEII G'GTnAC_C

Cuts at:    753     753 
   Size:       4205
 
BstXI CCAn_nnnn'nTGG

Cuts at:    312     525     639     312 
   Size:        213     114    3878

  Fragments arranged by size: 

                3878     213     114
 
BstYI r'GATC_y

Cuts at:      1    1079    1230    1697    1720    2055    2301    3313    3399 
   Size:       1078     151     467      23     335     246    1012      86

Cuts at:   3399    3411    3757       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1078    1012     467     449     346     335     246     151
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       4205
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2155    2336    2499    2155 
   Size:        181     163    3861

  Fragments arranged by size: 

                3861     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1104    1448    1104 
   Size:        344    3861
 
DraI TTT'AAA

Cuts at:   1204    1204 
   Size:       4205
 
DraIII CAC_nnn'GTG

Cuts at:   1194    1194 
   Size:       4205
 
DrdI GACnn_nn'nnGTC

Cuts at:   1911    2769    1911 
   Size:        858    3347
 
EaeI y'GGCC_r

Cuts at:    302     515     629    1790    1964    2355    2382    3727     302 
   Size:        213     114    1161     174     391      27    1345     780

  Fragments arranged by size: 

                1345    1161     780     391     213     174     114      27
            
 
EagI C'GGCC_G

Cuts at:   1790    1790 
   Size:       4205
 
EarI CTCTTCn'nnn_

Cuts at:   2228    2438    2228 
   Size:        210    3995
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    864    2413    2723    2869    4015     864 
   Size:       1549     310     146    1146    1054

  Fragments arranged by size: 

                1549    1146    1054     310     146
 
EcoICRI GAG'CTC

Cuts at:   1217    1217 
   Size:       4205
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    898    1190    2030    2462    3209    3477     898 
   Size:        292     840     432     747     268    1626

  Fragments arranged by size: 

                1626     840     747     432     292     268
 
EcoNI CCTnn'n_nnAGG

Cuts at:    478     478 
   Size:       4205
 
EcoO109I rG'GnC_Cy

Cuts at:    436     483     902    1344     436 
   Size:         47     419     442    3297

  Fragments arranged by size: 

                3297     442     419      47
 
EcoRI G'AATT_C

Cuts at:   1009    1209    1009 
   Size:        200    4005
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2224    2224 
   Size:       4205
 
FspI TGC'GCA

Cuts at:   1986    1986 
   Size:       4205
 
HaeII r_GCGC'y

Cuts at:   1067    1639    1887    2909    3888    1067 
   Size:        572     248    1022     979    1384

  Fragments arranged by size: 

                1384    1022     979     572     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    260     292    2047    2077    2079    2109     260 
   Size:         32    1755      30       2      30    2356

  Fragments arranged by size: 

                2356    1755      32      30      30       2
 
HincII GTy'rAC

Cuts at:    336    1244     336 
   Size:        908    3297
 
KasI G'GCGC_C

Cuts at:   1635    1883    1635 
   Size:        248    3957
 
KpnI G_GTAC'C

Cuts at:   1225    1225 
   Size:       4205
 
MluI A'CGCG_T

Cuts at:   3638    3638 
   Size:       4205
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    719    2876    3060     719 
   Size:       2157     184    1864

  Fragments arranged by size: 

                2157    1864     184
 
MscI TGG'CCA

Cuts at:    304     517     631    1966     304 
   Size:        213     114    1335    2543

  Fragments arranged by size: 

                2543    1335     213     114
 
MslI CAynn'nnrTG

Cuts at:    160     580     637    1181    2194    2321     160 
   Size:        420      57     544    1013     127    2044

  Fragments arranged by size: 

                2044    1013     544     420     127      57
 
MspA1I CmG'CkG

Cuts at:   1316    1630    1990    3003    3248    1316 
   Size:        314     360    1013     245    2273

  Fragments arranged by size: 

                2273    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   2386    2386 
   Size:       4205
 
NarI GG'CG_CC

Cuts at:   1636    1884    1636 
   Size:        248    3957
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       4205
 
NgoMIV G'CCGG_C

Cuts at:   2384    2384 
   Size:       4205
 
NspI r_CATG'y

Cuts at:    567    1258    1758    2665     567 
   Size:        691     500     907    2107

  Fragments arranged by size: 

                2107     907     691     500
 
PciI A'CATG_T

Cuts at:   2661    2661 
   Size:       4205
 
PflMI CCAn_nnn'nTGG

Cuts at:    239    1194     239 
   Size:        955    3250
 
PfoI T'CCnGG_A

Cuts at:    924     924 
   Size:       4205
 
PmeI GTTT'AAAC

Cuts at:   1204    1204 
   Size:       4205
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3344    3376    3344 
   Size:         32    4173
 
PpuMI rG'GwC_Cy

Cuts at:    436     436 
   Size:       4205
 
PshAI GACnn'nnGTC

Cuts at:    886     886 
   Size:       4205
 
PsiI TTA'TAA

Cuts at:    209     209 
   Size:       4205
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:    758     790     758 
   Size:         32    4173
 
PstI C_TGCA'G

Cuts at:    390    1252    1937     390 
   Size:        862     685    2658

  Fragments arranged by size: 

                2658     862     685
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4205
 
PvuII CAG'CTG

Cuts at:   1630    1990    1630 
   Size:        360    3845
 
RsrII CG'GwC_CG

Cuts at:   2400    2400 
   Size:       4205
 
SacI G_AGCT'C

Cuts at:   1219    1219 
   Size:       4205
 
SalI G'TCGA_C

Cuts at:   1242    1242 
   Size:       4205
 
SapI GCTCTTCn'nnn_

Cuts at:   2228    2438    2228 
   Size:        210    3995
 
SbfI CC_TGCA'GG

Cuts at:   1252    1252 
   Size:       4205
 
ScaI AGT'ACT

Cuts at:   3722    3722 
   Size:       4205
 
SfcI C'TryA_G

Cuts at:     33     386     711    1248    1933    2926    3117      33 
   Size:        353     325     537     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     537     353     325     191
 
SfoI GGC'GCC

Cuts at:   1637    1885    1637 
   Size:        248    3957
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4205
 
SmaI CCC'GGG

Cuts at:   1227    1227 
   Size:       4205
 
SmlI C'TyrA_G

Cuts at:   1360    2275    2767    3029    1360 
   Size:        915     492     262    2536

  Fragments arranged by size: 

                2536     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4205
 
SphI G_CATG'C

Cuts at:    567    1258    1758     567 
   Size:        691     500    3014

  Fragments arranged by size: 

                3014     691     500
 
StyI C'CwwG_G

Cuts at:    107    1339    1693     107 
   Size:       1232     354    2619

  Fragments arranged by size: 

                2619    1232     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    631    1788    1921    2380    2589     631 
   Size:       1157     133     459     209    2247

  Fragments arranged by size: 

                2247    1157     459     209     133
 
TatI w'GTAC_w

Cuts at:   3720    3720 
   Size:       4205
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3722    3754    3722 
   Size:         32    4173
 
Tth111I GACn'n_nGTC

Cuts at:   2002    2002 
   Size:       4205
 
XbaI T'CTAG_A

Cuts at:    945    1236     945 
   Size:        291    3914
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    747    1700     747 
   Size:        953    3252
 
XmaI C'CCGG_G

Cuts at:   1225    1225 
   Size:       4205
 
XmnI GAAnn'nnTTC

Cuts at:    991     991 
   Size:       4205
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI     BcgI    BciVI     BclI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI     BtgI    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NspI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI
     PsiI     PsrI     PstI     PvuI    PvuII    RsrII     SacI     SalI
     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI
     SpeI     SphI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI    BbvCI    BfrBI     BglI     BmtI
   Bpu10I    BsaXI    BseRI     BsgI    BsiWI     BsmI    BsmBI    BsrGI
   BssHII  BstZ17I   Bsu36I     ClaI    CspCI    EcoRV     FseI    FspAI
  HindIII     HpaI     MfeI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PasI     PmlI   PspOMI    PspXI    SacII    SanDI    SexAI
     SfiI    SgrAI    SnaBI     SrfI     SspI     StuI     SwaI     XhoI
     ZraI
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