##################################
# NO CUT ENZYMES (  188 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AocI        Aor51HI     ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AvaI        AviII       AvrII       BalI        
BamHI       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsmAI       BsmBI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrGI       BsrI        BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstF5I      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DpnII       
DraI        DraIII      DrdI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FbaI        FokI        FseI        FspI        
HgaI        Hin1I       HincII      HindII      HindIII     
HinfI       HpaI        HphI        KasI        KpnI        
Ksp632I     KspI        MaeIII      MamI        MboI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MseI        MunI        MvnI        NarI        
NcoI        NdeI        NdeII       NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsaI        RsrII       SacI        
SacII       SalI        SapI        Sau3AI      SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StyI        
SwaI        TfiI        Tru9I       Tsp45I      Tsp509I     
TspRI       Van91I      XbaI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   22 enzymes) #
###################################

AlwNI         424  CAGCGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AspI          213  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BcnI          329  CCCGG                CC^[GC]GG
BfaI          232  CTAG                 C^TAG
BpmI          131  CTGGAG               CTGGAG(16/14)
BseRI         253  GAGGAG               GAGGAG(10/8)
BstAPI        423  GCAGCGGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DdeI          249  CTCAG                C^T[ACGT]AG
HaeII         321  GGCGCT               [GA]GCGC^[CT]
MaeI          232  CTAG                 C^TAG
MaeII         392  ACGT                 A^CGT
MboII         266  TCTTC                GAAGA(8/7)
MslI          387  CACCGACGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI          329  CCCGG                CC^[GC]GG
PflFI         213  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI         136  GGGACCT              [GA]G^G[AT]CC[CT]
SfaNI          73  GATGC                GCATC(5/9)
StuI          234  AGGCCT               AGG^CCT
TaqI          404  TCGA                 T^CGA
Tth111I       213  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       404  TCGA                 T^CGA
XcmI          193  CCATGCCAGCTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

ApaI           66  GGGCCC               GGGCC^C
              361  GGGCCC              
AvaII         137  GGACC                G^G[AT]CC
              190  GGTCC               
BanI          148  GGCACC               G^G[CT][GA]CC
              298  GGTGCC              
BsrFI         280  GCCGGC               [GA]^CCGG[CT]
              371  GCCGGC              
Cfr10I        280  GCCGGC               [GA]^CCGG[CT]
              371  GCCGGC              
EaeI            2  TGGCCG               [CT]^GGCC[GA]
               92  TGGCCG              
NaeI          280  GCCGGC               GCC^GGC
              371  GCCGGC              
NgoMIV        280  GCCGGC               G^CCGGC
              371  GCCGGC              
NlaIII        194  CATG                 CATG^
              313  CATG                
PstI           46  CTGCAG               CTGCA^G
              334  CTGCAG              
SfcI           46  CTGCAG               C^T[GA][CT]AG
              334  CTGCAG              


#########################################
# 3 OR MORE CUT ENZYMES (   37 enzymes) #
#########################################

AciI        AluI        BanII       BbvI        BmyI        
BsaJI       BsiYI       BslI        BsmFI       Bsp1286I    
BssKI       BstNI       Cac8I       CfoI        Cfr13I      
DraII       DsaI        EcoO109I    EcoRII      FauI        
Fnu4HI      HaeIII      HapII       HhaI        HinP1I      
HpaII       ItaI        MnlI        MspA1I      MspI        
MvaI        MwoI        NlaIV       PvuII       Sau96I      
ScrFI       TseI