Restriction map for pF1KE0592
##################################
# NO CUT ENZYMES (  112 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AvaI        AviII       BbvCI       BcgI        
BciVI       BfrI        BlpI        Bpu1102I    BsaBI       
BseAI       BseRI       BsgI        BsiEI       BsiWI       
BsmBI       BsoBI       Bsp1407I    BspDI       BspEI       
BspLU11I    BsrBI       BsrGI       BssHII      BssSI       
BstAPI      BstBI       BstEII      BstPI       BstXI       
Bsu36I      CelII       ClaI        CpoI        DraI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoO65I     EcoRI       
EcoRV       EcoT22I     FseI        FspI        HgaI        
HindIII     HpaI        KpnI        MamI        MfeI        
MluI        MroI        MunI        NaeI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PleI        
PmeI        PshAI       PshBI       Psp1406I    PvuI        
RsrII       SalI        SbfI        SfiI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TaqI        TthHB8I     XbaI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccI         1087  GTATAC               GT^[AC][GT]AC
AccII        1260  CGCG                 CG^CG
AflIII        977  ACACGT               A^C[GA][CT]GT
AgeI         1241  ACCGGT               A^CCGGT
Asp700I       579  GAAGGCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         326  GAGCTC               G[AT]GC[AT]^C
AvrII        1311  CCTAGG               C^CTAGG
BamHI        1337  GGATCC               G^GATCC
BbrPI         998  CACGTG               CAC^GTG
BclI          362  TGATCA               T^GATCA
BglI          956  GCCTGCTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1376  AGATCT               A^GATCT
BlnI         1311  CCTAGG               C^CTAGG
BpmI         1040  CTCCAG               CTGGAG(16/14)
BsaAI         998  CACGTG               [CT]AC^GT[GA]
BsaWI        1241  ACCGGT               [AT]^CCGG[AT]
BsiHKAI       326  GAGCTC               G[AT]GC[AT]^C
BspHI         725  TCATGA               T^CATGA
BspMI         791  ACCTGC               ACCTGC(4/8)
BsrDI         546  CATTGC               GCAATG(2/0)
BsrFI        1241  ACCGGT               [GA]^CCGG[CT]
Bst1107I     1087  GTATAC               GTA^TAC
BstUI        1260  CGCG                 CG^CG
BstZ17I      1087  GTATAC               GTA^TAC
Cfr10I       1241  ACCGGT               [GA]^CCGG[CT]
EcoNI         687  CCTTCGGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI          809  CCCGC                CCCGC(4/6)
FbaI          362  TGATCA               T^GATCA
HincII        601  GTCAAC               GT[CT]^[GA]AC
HindII        601  GTCAAC               GT[CT]^[GA]AC
KspI         1259  CCGCGG               CCGC^GG
MslI          222  CACATTGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI         1260  CGCG                 CG^CG
NcoI         1223  CCATGG               C^CATGG
PinAI        1241  ACCGGT               A^CCGGT
PmaCI         998  CACGTG               CAC^GTG
PmlI          998  CACGTG               CAC^GTG
PpuMI         413  GGGACCC              [GA]G^G[AT]CC[CT]
PstI          877  CTGCAG               CTGCA^G
RcaI          725  TCATGA               T^CATGA
SacI          326  GAGCTC               GAGCT^C
SacII        1259  CCGCGG               CCGC^GG
ScaI         1367  AGTACT               AGT^ACT
SexAI         967  ACCTGGT              A^CC[AT]GGT
SfaNI        1280  GCATC                GCATC(5/9)
SfcI          877  CTGCAG               C^T[GA][CT]AG
XcmI          345  CCAAAAGTTATTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          579  GAAGGCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AcyI          286  GGCGCC               G[GA]^CG[CT]C
             1315  GGCGCC              
AspI          673  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1069  GACCCGGTC           
BalI          659  TGGCCA               TGG^CCA
              819  TGGCCA              
BbeI          286  GGCGCC               GGCGC^C
             1315  GGCGCC              
BsaHI         286  GGCGCC               G[GA]^CG[CT]C
             1315  GGCGCC              
BsaI         1216  GAGACC               GGTCTC(1/5)
             1143  GGTCTC              
BsmAI        1216  GAGAC                GTCTC(1/5)
             1144  GTCTC               
BsmFI         413  GGGAC                GGGAC(10/14)
             1035  GTCCC               
BsmI          583  GCATTC               GAATGC(1/-1)
             1117  GCATTC              
CfoI          287  GCGC                 GCG^C
             1316  GCGC                
HaeII         286  GGCGCC               [GA]GCGC^[CT]
             1315  GGCGCC              
HhaI          287  GCGC                 GCG^C
             1316  GCGC                
Hin1I         286  GGCGCC               G[GA]^CG[CT]C
             1315  GGCGCC              
HinP1I        287  GCGC                 G^CGC
             1316  GCGC                
KasI          286  GGCGCC               G^GCGCC
             1315  GGCGCC              
MluNI         659  TGGCCA               TGG^CCA
              819  TGGCCA              
MscI          659  TGGCCA               TGG^CCA
              819  TGGCCA              
NarI          286  GGCGCC               GG^CGCC
             1315  GGCGCC              
PflFI         673  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1069  GACCCGGTC           
PvuII         173  CAGCTG               CAG^CTG
             1177  CAGCTG              
SapI          846  GCTCTTC              GCTCTTC(1/4)
             1422  GCTCTTC             
SfoI          286  GGCGCC               GGC^GCC
             1315  GGCGCC              
Tth111I       673  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1069  GACCCGGTC           


#########################################
# 3 OR MORE CUT ENZYMES (   75 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApaI        ApoI        AvaII       BanI        BanII       
BbsI        BbvI        BcnI        BfaI        BmyI        
BpuAI       BsaJI       BsiYI       BslI        Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        EcoO109I    EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HapII       
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PflMI       RsaI        Sau3AI      Sau96I      
ScrFI       StyI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       Van91I      XhoII       
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