Restriction map for pF1KE0592
 (Linear) (Six-base) MAPSORT of: KIEE0592.seq  Check: 6333  from: 1  to: 1428



KIEE0592.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:16  ..

AccI GT'mk_AC

Cuts at:      0    1088    1428 
   Size:       1088     340
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     149     918    1201    1428 
   Size:        149     769     283     227

  Fragments arranged by size: 

                 769     283     227     149
 
AflIII A'CryG_T

Cuts at:      0     977    1428 
   Size:        977     451
 
AgeI A'CCGG_T

Cuts at:      0    1241    1428 
   Size:       1241     187
 
ApaI G_GGCC'C

Cuts at:      0     994    1290    1419    1428 
   Size:        994     296     129       9

  Fragments arranged by size: 

                 994     296     129       9
 
ApoI r'AATT_y

Cuts at:      0      10      53     437     622    1428 
   Size:         10      43     384     185     806

  Fragments arranged by size: 

                 806     384     185      43      10
 
AvrII C'CTAG_G

Cuts at:      0    1311    1428 
   Size:       1311     117
 
BamHI G'GATC_C

Cuts at:      0    1337    1428 
   Size:       1337      91
 
BanI G'GyrC_C

Cuts at:      0     286     964    1315    1428 
   Size:        286     678     351     113

  Fragments arranged by size: 

                 678     351     286     113
 
BanII G_rGCy'C

Cuts at:      0     330     345     687     994    1111    1290    1419    1428 
   Size:        330      15     342     307     117     179     129       9

  Fragments arranged by size: 

                 342     330     307     179     129     117      15       9
            
 
BbeI G_GCGC'C

Cuts at:      0     290    1319    1428 
   Size:        290    1029     109

  Fragments arranged by size: 

                1029     290     109
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     155     974    1073    1428 
   Size:        155     819      99     355

  Fragments arranged by size: 

                 819     355     155      99
 
BclI T'GATC_A

Cuts at:      0     362    1428 
   Size:        362    1066
 
BglI GCCn_nnn'nGGC

Cuts at:      0     962    1428 
   Size:        962     466
 
BglII A'GATC_T

Cuts at:      0    1376    1428 
   Size:       1376      52
 
Bme1580I G_kGCm'C

Cuts at:      0     967     994    1290    1419    1428 
   Size:        967      27     296     129       9

  Fragments arranged by size: 

                 967     296     129      27       9
 
BmgBI CAC'GTC

Cuts at:      0     257     980    1428 
   Size:        257     723     448

  Fragments arranged by size: 

                 723     448     257
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1025    1428 
   Size:       1025     403
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1149    1210    1428 
   Size:       1149      61     218

  Fragments arranged by size: 

                1149     218      61
 
BsaAI yAC'GTr

Cuts at:      0    1000    1428 
   Size:       1000     428
 
BsaHI Gr'CG_yC

Cuts at:      0     287    1316    1428 
   Size:        287    1029     112

  Fragments arranged by size: 

                1029     287     112
 
BsaWI w'CCGG_w

Cuts at:      0    1241    1428 
   Size:       1241     187
 
BseYI C'CCAG_C

Cuts at:      0     960     993    1104    1428 
   Size:        960      33     111     324

  Fragments arranged by size: 

                 960     324     111      33
 
BsiHKAI G_wGCw'C

Cuts at:      0     330    1428 
   Size:        330    1098
 
BsmI GAATG_Cn'

Cuts at:      0     583    1117    1428 
   Size:        583     534     311

  Fragments arranged by size: 

                 583     534     311
 
Bsp1286I G_dGCh'C

Cuts at:      0     330     345     687     967     994    1111    1290    1419 
   Size:        330      15     342     280      27     117     179     129

Cuts at:   1419    1428 
   Size:          9

  Fragments arranged by size: 

                 342     330     280     179     129     117      27      15
                   9
 
BspHI T'CATG_A

Cuts at:      0     725    1428 
   Size:        725     703
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     800    1428 
   Size:        800     628
 
BsrDI GCAATG_nn'

Cuts at:      0     545    1428 
   Size:        545     883
 
BsrFI r'CCGG_y

Cuts at:      0    1241    1428 
   Size:       1241     187
 
BstYI r'GATC_y

Cuts at:      0     239    1337    1376    1428 
   Size:        239    1098      39      52

  Fragments arranged by size: 

                1098     239      52      39
 
BstZ17I GTA'TAC

Cuts at:      0    1089    1428 
   Size:       1089     339
 
BtgI C'CryG_G

Cuts at:      0     816    1223    1259    1428 
   Size:        816     407      36     169

  Fragments arranged by size: 

                 816     407     169      36
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     624     720    1428 
   Size:        624      96     708

  Fragments arranged by size: 

                 708     624      96
 
BtsI GCAGTG_nn'

Cuts at:      0     764    1428 
   Size:        764     664
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     729     764    1428 
   Size:        729      35     664

  Fragments arranged by size: 

                 729     664      35
 
EaeI y'GGCC_r

Cuts at:      0     375     609     659     819    1428 
   Size:        375     234      50     160     609

  Fragments arranged by size: 

                 609     375     234     160      50
 
EarI CTCTTCn'nnn_

Cuts at:      0     337     547     853    1428 
   Size:        337     210     306     575

  Fragments arranged by size: 

                 575     337     306     210
 
EcoICRI GAG'CTC

Cuts at:      0     328    1428 
   Size:        328    1100
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     149     918    1025    1201    1428 
   Size:        149     769     107     176     227

  Fragments arranged by size: 

                 769     227     176     149     107
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     691    1428 
   Size:        691     737
 
EcoO109I rG'GnC_Cy

Cuts at:      0     414     990    1286    1287    1415    1416    1428 
   Size:        414     576     296       1     128       1      12

  Fragments arranged by size: 

                 576     414     296     128      12       1       1
 
HaeII r_GCGC'y

Cuts at:      0     290    1319    1428 
   Size:        290    1029     109

  Fragments arranged by size: 

                1029     290     109
 
HincII GTy'rAC

Cuts at:      0     603    1428 
   Size:        603     825
 
KasI G'GCGC_C

Cuts at:      0     286    1315    1428 
   Size:        286    1029     113

  Fragments arranged by size: 

                1029     286     113
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     884     920     953    1428 
   Size:        884      36      33     475

  Fragments arranged by size: 

                 884     475      36      33
 
MscI TGG'CCA

Cuts at:      0     661     821    1428 
   Size:        661     160     607

  Fragments arranged by size: 

                 661     607     160
 
MslI CAynn'nnrTG

Cuts at:      0     226    1428 
   Size:        226    1202
 
MspA1I CmG'CkG

Cuts at:      0     175    1179    1261    1428 
   Size:        175    1004      82     167

  Fragments arranged by size: 

                1004     175     167      82
 
NarI GG'CG_CC

Cuts at:      0     287    1316    1428 
   Size:        287    1029     112

  Fragments arranged by size: 

                1029     287     112
 
NcoI C'CATG_G

Cuts at:      0    1223    1428 
   Size:       1223     205
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     575     657    1015    1133    1428 
   Size:        575      82     358     118     295

  Fragments arranged by size: 

                 575     358     295     118      82
 
PmlI CAC'GTG

Cuts at:      0    1000    1428 
   Size:       1000     428
 
PpuMI rG'GwC_Cy

Cuts at:      0     414    1428 
   Size:        414    1014
 
PsiI TTA'TAA

Cuts at:      0     268    1428 
   Size:        268    1160
 
PspOMI G'GGCC_C

Cuts at:      0     990    1286    1415    1428 
   Size:        990     296     129      13

  Fragments arranged by size: 

                 990     296     129      13
 
PstI C_TGCA'G

Cuts at:      0     881    1428 
   Size:        881     547
 
PvuII CAG'CTG

Cuts at:      0     175    1179    1428 
   Size:        175    1004     249

  Fragments arranged by size: 

                1004     249     175
 
SacI G_AGCT'C

Cuts at:      0     330    1428 
   Size:        330    1098
 
SacII CC_GC'GG

Cuts at:      0    1262    1428 
   Size:       1262     166
 
SanDI GG'GwC_CC

Cuts at:      0     414    1428 
   Size:        414    1014
 
SapI GCTCTTCn'nnn_

Cuts at:      0     853    1428 
   Size:        853     575
 
ScaI AGT'ACT

Cuts at:      0    1369    1428 
   Size:       1369      59
 
SexAI A'CCwGG_T

Cuts at:      0     967    1428 
   Size:        967     461
 
SfcI C'TryA_G

Cuts at:      0     877    1428 
   Size:        877     551
 
SfoI GGC'GCC

Cuts at:      0     288    1317    1428 
   Size:        288    1029     111

  Fragments arranged by size: 

                1029     288     111
 
StyI C'CwwG_G

Cuts at:      0     522    1223    1311    1428 
   Size:        522     701      88     117

  Fragments arranged by size: 

                 701     522     117      88
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     338     418    1284    1428 
   Size:        338      80     866     144

  Fragments arranged by size: 

                 866     338     144      80
 
TatI w'GTAC_w

Cuts at:      0     824    1367    1428 
   Size:        824     543      61

  Fragments arranged by size: 

                 824     543      61
 
Tth111I GACn'n_nGTC

Cuts at:      0     676    1072    1428 
   Size:        676     396     356

  Fragments arranged by size: 

                 676     396     356
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     352    1428 
   Size:        352    1076
 
XmnI GAAnn'nnTTC

Cuts at:      0     583    1428 
   Size:        583     845
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AgeI     ApaI     ApoI    AvrII    BamHI
     BanI    BanII     BbeI     BbsI     BclI     BglI    BglII Bme1580I
    BmgBI     BpmI     BsaI    BsaAI    BsaHI    BsaWI    BseYI  BsiHKAI
     BsmI Bsp1286I    BspHI    BspMI    BsrDI    BsrFI    BstYI  BstZ17I
     BtgI    BtgZI     BtsI    CspCI     EaeI     EarI  EcoICRI  Eco57MI
    EcoNI EcoO109I    HaeII   HincII     KasI     MmeI     MscI     MslI
   MspA1I     NarI     NcoI    PflMI     PmlI    PpuMI     PsiI   PspOMI
     PstI    PvuII     SacI    SacII    SanDI     SapI     ScaI    SexAI
     SfcI     SfoI     StyI    TaqII     TatI  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AhdI     AleI
     AlfI     AloI    AlwNI    ApaLI     AscI     AseI    AsiSI     AvaI
     BaeI    BbvCI     BcgI    BciVI    BfrBI     BlpI     BmrI     BmtI
     BplI   Bpu10I    BpuEI    BsaBI    BsaXI    BseRI     BsgI    BsiEI
    BsiWI    BsmBI    BspEI    BsrBI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI   Bsu36I     ClaI     DraI   DraIII     DrdI
     EagI     EciI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    Hin4I  HindIII     HpaI     KpnI     MfeI     MluI     NaeI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PfoI     PmeI     PpiI    PshAI    PspXI     PsrI     PvuI
    RsrII     SalI     SbfI     SfiI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI     TstI
     XbaI     XhoI     XmaI     ZraI
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