Restriction map for pF1KE0583
##################################
# NO CUT ENZYMES (  155 enzymes) #
##################################

Acc65I      AccI        AccII       AccIII      AclI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwNI       Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BbeI        BbrPI       BbvCI       BcgI        
BcnI        BfrI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BsaAI       BsaBI       BsaWI       
BseAI       BsgI        BsiEI       BsiWI       BsoBI       
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrDI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstF5I      BstPI       BstUI       
BstXI       BstZ17I     CelII       CfoI        ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT22I     FokI        
FseI        FspI        HaeII       HhaI        HinP1I      
HincII      HindII      HindIII     HpaI        KasI        
KpnI        KspI        MamI        MfeI        MluI        
MluNI       MroI        MscI        MseI        MunI        
MvnI        NarI        NciI        NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SfaNI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspI        StuI        SwaI        TaqI        
Tru9I       Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AatII         693  GACGTC               GACGT^C
AcsI          413  GAATTT               [GA]^AATT[CT]
AcyI          693  GACGTC               G[GA]^CG[CT]C
AlwI           90  GATCC                GGATC(4/5)
AocI          595  CCTCAGG              CC^T[ACGT]AGG
ApoI          413  GAATTT               [GA]^AATT[CT]
AspHI          64  GAGCTC               G[AT]GC[AT]^C
BanI           45  GGTGCC               G^G[CT][GA]CC
BbsI          519  GAAGAC               GAAGAC(2/6)
BciVI         463  GGATAC               GTATCC(6/5)
BclI          749  TGATCA               T^GATCA
BfaI          212  CTAG                 C^TAG
BglI          605  GCCCCGAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI         519  GAAGAC               GAAGAC(2/6)
BsaHI         693  GACGTC               G[GA]^CG[CT]C
BsaI          565  GAGACC               GGTCTC(1/5)
BseRI         760  CTCCTC               GAGGAG(10/8)
BsiHKAI        64  GAGCTC               G[AT]GC[AT]^C
BsiYI         779  CCTGGCAGCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          779  CCTGGCAGCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI         691  GAGACG               CGTCTC(1/5)
BsmI          148  GAATGC               GAATGC(1/-1)
Bsp1407I      199  TGTACA               T^GTACA
BspMI          11  ACCTGC               ACCTGC(4/8)
BsrFI         730  GCCGGC               [GA]^CCGG[CT]
BsrGI         199  TGTACA               T^GTACA
BstYI          89  AGATCC               [GA]^GATC[CT]
Bsu36I        595  CCTCAGG              CC^T[ACGT]AGG
Cfr10I        730  GCCGGC               [GA]^CCGG[CT]
DraII         483  AGGTCCT              [GA]G^G[ACGT]CC[CT]
EaeI          728  TGGCCG               [CT]^GGCC[GA]
Eco81I        595  CCTCAGG              CC^T[ACGT]AGG
EcoO109I      483  AGGTCCT              [GA]G^G[ACGT]CC[CT]
FbaI          749  TGATCA               T^GATCA
HapII         731  CCGG                 C^CGG
Hin1I         693  GACGTC               G[GA]^CG[CT]C
HinfI           4  GATTC                G^A[ACGT]TC
HpaII         731  CCGG                 C^CGG
MaeI          212  CTAG                 C^TAG
MflI           89  AGATCC               [GA]^GATC[CT]
MspA1I        784  CAGCGG               C[AC]G^C[GT]G
MspI          731  CCGG                 C^CGG
NaeI          730  GCCGGC               GCC^GGC
NdeI          546  CATATG               CA^TATG
NgoMIV        730  GCCGGC               G^CCGGC
PpuMI         483  AGGTCCT              [GA]G^G[AT]CC[CT]
PstI          372  CTGCAG               CTGCA^G
SacI           64  GAGCTC               GAGCT^C
SexAI         716  ACCAGGT              A^CC[AT]GGT
SfcI          372  CTGCAG               C^T[GA][CT]AG
SspBI         199  TGTACA               T^GTACA
TfiI            4  GATTC                G^A[AT]TC
Tsp509I       414  AATT                 ^AATT
XhoII          89  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AfaI          200  GTAC                 GT^AC
              714  GTAC                
BsmFI          24  GGGAC                GGGAC(10/14)
              227  GGGAC               
BsrI          343  CCAGT                ACTGG(1/-1)
              569  CCAGT               
DpnII          90  GATC                 ^GATC
              750  GATC                
EarI          586  GAAGAG               CTCTTC(1/4)
              798  GAAGAG              
FauI           81  CCCGC                CCCGC(4/6)
              179  GCGGG               
Ksp632I       586  GAAGAG               CTCTTC(1/4)
              798  GAAGAG              
MaeII          34  ACGT                 A^CGT
              694  ACGT                
MboI           90  GATC                 ^GATC
              750  GATC                
MslI           39  CATCATGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              318  CACAGAAATG          
MwoI          605  GCCCCGAAGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              774  GCTCACCTGGC         
NdeII          90  GATC                 ^GATC
              750  GATC                
RsaI          200  GTAC                 GT^AC
              714  GTAC                
Sau3AI         90  GATC                 ^GATC
              750  GATC                


#########################################
# 3 OR MORE CUT ENZYMES (   35 enzymes) #
#########################################

AciI        AluI        AvaII       BanII       BbvI        
BmyI        BsaJI       BsmAI       Bsp1286I    BssKI       
BstNI       Cac8I       Cfr13I      DdeI        DsaI        
EcoRII      EcoT14I     Fnu4HI      HaeIII      HgaI        
HphI        ItaI        MaeIII      MboII       MnlI        
MvaI        NcoI        NlaIII      NlaIV       Sau96I      
ScrFI       StyI        TseI        Tsp45I      TspRI       
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