Restriction map for pF1KE0583
 (Linear) (Six-base) MAPSORT of: KIEE0583.seq  Check: 6579  from: 1  to: 816



KIEE0583.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:16  ..

AatII G_ACGT'C

Cuts at:      0     697     816 
   Size:        697     119
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     314     816 
   Size:        314     502
 
ApoI r'AATT_y

Cuts at:      0     413     816 
   Size:        413     403
 
BanI G'GyrC_C

Cuts at:      0      45     816 
   Size:         45     771
 
BanII G_rGCy'C

Cuts at:      0      68     474     776     816 
   Size:         68     406     302      40

  Fragments arranged by size: 

                 406     302      68      40
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     526     816 
   Size:        526     290
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     457     816 
   Size:        457     359
 
BclI T'GATC_A

Cuts at:      0     749     816 
   Size:        749      67
 
BglI GCCn_nnn'nGGC

Cuts at:      0     611     816 
   Size:        611     205
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     559     816 
   Size:        559     257
 
BsaHI Gr'CG_yC

Cuts at:      0     694     816 
   Size:        694     122
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     751     816 
   Size:        751      65
 
BseYI C'CCAG_C

Cuts at:      0     533     539     816 
   Size:        533       6     277

  Fragments arranged by size: 

                 533     277       6
 
BsiHKAI G_wGCw'C

Cuts at:      0      68     816 
   Size:         68     748
 
BsmI GAATG_Cn'

Cuts at:      0     154     816 
   Size:        154     662
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     685     816 
   Size:        685     131
 
Bsp1286I G_dGCh'C

Cuts at:      0      68     474     776     816 
   Size:         68     406     302      40

  Fragments arranged by size: 

                 406     302      68      40
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      20     816 
   Size:         20     796
 
BsrFI r'CCGG_y

Cuts at:      0     730     816 
   Size:        730      86
 
BsrGI T'GTAC_A

Cuts at:      0     199     816 
   Size:        199     617
 
BstYI r'GATC_y

Cuts at:      0      89     816 
   Size:         89     727
 
Bsu36I CC'TnA_GG

Cuts at:      0     596     816 
   Size:        596     220
 
BtgI C'CryG_G

Cuts at:      0     113     388     513     686     816 
   Size:        113     275     125     173     130

  Fragments arranged by size: 

                 275     173     130     125     113
 
BtsI GCAGTG_nn'

Cuts at:      0     369     731     816 
   Size:        369     362      85

  Fragments arranged by size: 

                 369     362      85
 
EaeI y'GGCC_r

Cuts at:      0     728     816 
   Size:        728      88
 
EarI CTCTTCn'nnn_

Cuts at:      0     581     793     816 
   Size:        581     212      23

  Fragments arranged by size: 

                 581     212      23
 
EcoICRI GAG'CTC

Cuts at:      0      66     816 
   Size:         66     750
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     314     816 
   Size:        314     502
 
EcoO109I rG'GnC_Cy

Cuts at:      0     484     816 
   Size:        484     332
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     376     816 
   Size:        376     440
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     402     434     816 
   Size:        402      32     382

  Fragments arranged by size: 

                 402     382      32
 
MslI CAynn'nnrTG

Cuts at:      0      43     322     816 
   Size:         43     279     494

  Fragments arranged by size: 

                 494     279      43
 
MspA1I CmG'CkG

Cuts at:      0     786     816 
   Size:        786      30
 
NaeI GCC'GGC

Cuts at:      0     732     816 
   Size:        732      84
 
NcoI C'CATG_G

Cuts at:      0     113     388     686     816 
   Size:        113     275     298     130

  Fragments arranged by size: 

                 298     275     130     113
 
NdeI CA'TA_TG

Cuts at:      0     547     816 
   Size:        547     269
 
NgoMIV G'CCGG_C

Cuts at:      0     730     816 
   Size:        730      86
 
PpuMI rG'GwC_Cy

Cuts at:      0     484     816 
   Size:        484     332
 
PstI C_TGCA'G

Cuts at:      0     376     816 
   Size:        376     440
 
SacI G_AGCT'C

Cuts at:      0      68     816 
   Size:         68     748
 
SexAI A'CCwGG_T

Cuts at:      0     716     816 
   Size:        716     100
 
SfcI C'TryA_G

Cuts at:      0     372     816 
   Size:        372     444
 
StyI C'CwwG_G

Cuts at:      0     113     388     686     816 
   Size:        113     275     298     130

  Fragments arranged by size: 

                 298     275     130     113
 
TatI w'GTAC_w

Cuts at:      0     199     816 
   Size:        199     617
 
ZraI GAC'GTC

Cuts at:      0     695     816 
   Size:        695     121
 

 Enzymes that do cut:

    AatII     AcuI     ApoI     BanI    BanII     BbsI    BciVI     BclI
     BglI     BsaI    BsaHI    BseRI    BseYI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspMI    BsrFI    BsrGI    BstYI   Bsu36I     BtgI     BtsI
     EaeI     EarI  EcoICRI  Eco57MI EcoO109I     FalI    Hin4I     MslI
   MspA1I     NaeI     NcoI     NdeI   NgoMIV    PpuMI     PstI     SacI
    SexAI     SfcI     StyI     TatI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI    BbvCI
     BcgI    BfrBI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaWI    BsaXI
     BsgI    BsiEI    BsiWI    BspEI    BspHI    BsrBI    BsrDI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EciI    EcoNI    EcoRI
    EcoRV     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     NarI     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SapI
     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
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